Protein Alignment Rhau and DHX36

DIOPT Version :10

• Sequence 1: NP_610056.1
• Gene: Rhau / 35339
• FlyBaseID: FBgn0032883
• Length: 942
• Species: Drosophila melanogaster
• Sequence 2: NP_065916.2
• Gene: DHX36 / 170506
• HGNCID: 14410
• Length: 1008
• Species: Homo sapiens
• Alignment Length: 978
• Identity: 379/978 - (38%)
• Similarity: 569/978 - (58%)
• Gaps: 88/978 - (8%)

  Fly     9 GSNARKGNRPPGLRGKDIGLYYRNLARQQKK--DRGENA-----ESKEPQI-RLGCNVSAPSGVL 65
            |....:|..|..|:|::||::|.....|:.|  :|.|.|     |.:|.|| :|..:|.|.:
Human    45 GGRGGRGRHPGHLKGREIGMWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKN--- 106

  Fly    66 ERVKELM------EDY--------SRAPSRQN---VDDKNVDAKFQQQFRHLLSVNF----EEFV 109
            ::..|..      ||:        ...|..:|   :.:|.:..:.::.|| :.:.::    .|::
Human   107 DKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFR-IRNRSYIDRDSEYL 170

  Fly   110 AETKERNADLDWVNPKLDERLQLELGQRQLEENAKKRLEA--------RKKLPTMKYADDIIQAV 166
            .:..|.:..||               |:.||:..||:.:.        |:|||:.....:::..:
Human   171 LQENEPDGTLD---------------QKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNLI 220

  Fly   167 RENQVILIVGSTGCGKTTQVPQILLDDAISRGCASSCRIICTQPRRISAIAIAEWVSYERCESL- 230
            ..:||.:|.|.|||||||||.|.:||:.|.||..|:|||:||||||||||::||.|:.||.||.
Human   221 DNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCG 285

  Fly   231 -GNSVGYQIRLESRKARERASITYCTTGVLLQQLQSDPLMHNLSVLILDEIHERSVETDLLMGLL 294
             |||.||||||:||..|::.||.|||||::||.|||||.:.::|.::|||||||::::|:||.::
Human   286 SGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVV 350

  Fly   295 KVILPHRPDLKVILMSATVREQDFCDYFNNCPMFRIEGVMFPVKMLYLEDVLSKTNY-------- 351
            |.:|..|.||||||||||:..:.|.:||.||||..|.|..|||....||||:.|..|
Human   351 KDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHR 415

  Fly   352 -EFQK-FRDRRPKRDPPERRMKHEAMIE----PYLRRIRNSYDSRVLDKLRLPESEGCEDIDFIA 410
             :|:: |......|...|.:   ||:.:    .|:|.:|..|.:..:|.:.:.|.:.. |::.|.
Human   416 SQFKRGFMQGHVNRQEKEEK---EAIYKERWPDYVRELRRRYSASTVDVIEMMEDDKV-DLNLIV 476

  Fly   411 DLVYYICENEPEGAILVFLPGYDKISQLYNILDKPKTSKGQRWRDHMAVFPLHSLMQSGEQQAVF 475
            .|:.||...|.:|||||||||:|.||.|:::|......|.    |...:.||||||.:..|..||
Human   477 ALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKS----DKFLIIPLHSLMPTVNQTQVF 537

  Fly   476 RRPPAGQRKVIISTIIAETSVTIDDVVYVINSGRTKATNYDIETNIQSLDEVWVTKANTQQRRGR 540
            :|.|.|.||::|:|.|||||:|||||||||:.|:.|.|::|.:.||.::...||:|||.:||:||
Human   538 KRTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGR 602

  Fly   541 AGRVRPGICYNLFSRAREDRMDDIPTPEILRSKLESIILSLKLLHIDDPYRFLQTLINAPNPEAI 605
            ||||:||.||:|::..|...:||...|||||:.||.:.|.:|:|.:.....||..|::.|:.||:
Human   603 AGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAV 667

  Fly   606 KMGVELLKRIEALDQTGTLTPLGMHLAKLPIDPQMGKMILMSALFCCLDPITSAAAALSFKSPFY 670
            .:.:..|..:.|||:...|||||:|||:||::|.:|||||..||||||||:.:.||:||||.||.
Human   668 LLSIRHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFV 732

  Fly   671 SPLGKESRVDEIKRRMARNMRSDHLMVHNTIIAYRDSRYS--HAERDFCYKNFLSSMTLQQLERM 733
            .|||||...|..::.:|::.|||||.|.|....:.::|..  ..|:|:|::.||||.|||.|..|
Human   733 IPLGKEKIADARRKELAKDTRSDHLTVVNAFEGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNM 797

  Fly   734 KNQFSELLYNYKFLASSNCKDAASNKNSEKIPLLRAIIGAGLYPNMAHLRKSRQIKNRVRAIHTM 798
            |.||:|.|....|::|.|.||..||.||:...:::|:|.|||||.:|.:|.:...|.::..::|.
Human   798 KGQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTK 862

  Fly   799 ATDDGRRVNFHPSSVNSGESGFDSAYFVYFQRQKSTDLFLLDSTMVFPMALIIFGDGVEAGVTQN 863
            .  || .|..||.|||..::.|...:.:|..:.:::.::|.|.|.|.|..|:.||..:......:
Human   863 T--DG-LVAVHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDND 924

  Fly   864 TPYLCVAKTYYFKCNRETADVVIQLRSNLEKLLLKKALYPAPIEENGYEKQ---LIKAIELLLSL 925
            ...:.|.:...|:.....|.:|.:||..|:.||.:|...|.|::.|..:.:   ::.||..|:..
Human   925 QETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKT 989

  Fly   926 DER 928
            .|:
Human   990 QEK 992

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rhau	NP_610056.1	HrpA	160..>664	CDD:441249	245/519 (47%)
		OB_NTP_bind	767..856	CDD:400182	29/88 (33%)
DHX36	NP_065916.2	Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000269|PubMed:18279852	1..200		37/173 (21%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000250|UniProtKB:D4A2Z8	1..104		19/58 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..58		3/12 (25%)
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000269|PubMed:18854321	1..51		1/5 (20%)
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000269|PubMed:21149580, ECO:0000269|PubMed:21846770, ECO:0000269|PubMed:24151078	53..105		17/51 (33%)
		DSM (DHX36-specific motif). /evidence=ECO:0000269|PubMed:20472641, ECO:0000269|PubMed:22238380, ECO:0000269|PubMed:24369427, ECO:0000269|PubMed:26195789, ECO:0000269|PubMed:26649896	53..75		7/21 (33%)
		RecA-like domain 1. /evidence=ECO:0000250|UniProtKB:Q05B79	106..386		118/298 (40%)
		DEXHc_DHX36	207..386	CDD:350739	99/178 (56%)
		HrpA	215..>776	CDD:441249	266/568 (47%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	265..317		32/51 (63%)
		DEAH box. /evidence=ECO:0000255|PROSITE-ProRule:PRU00541	334..337		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250|UniProtKB:Q05B79	387..628		101/248 (41%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	498..557		26/62 (42%)
		Nuclear localization signal. /evidence=ECO:0000303|PubMed:14731398	517..528		5/10 (50%)
		WH domain. /evidence=ECO:0000250|UniProtKB:Q05B79	629..698		29/68 (43%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	638..697		21/58 (36%)
		OB_NTP_bind	831..917	CDD:400182	29/88 (33%)
		OB-fold-like subdomains. /evidence=ECO:0000250|UniProtKB:Q05B79	841..905		18/66 (27%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	849..860		1/10 (10%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	870..900		7/29 (24%)