Protein Alignment Rhau and DHX9

DIOPT Version :10

• Sequence 1: NP_610056.1
• Gene: Rhau / 35339
• FlyBaseID: FBgn0032883
• Length: 942
• Species: Drosophila melanogaster
• Sequence 2: NP_001348.2
• Gene: DHX9 / 1660
• HGNCID: 2750
• Length: 1270
• Species: Homo sapiens
• Alignment Length: 981
• Identity: 301/981 - (30%)
• Similarity: 485/981 - (49%)
• Gaps: 168/981 - (17%)

  Fly    25 DIGLYYRNLARQ-QKKDRGENAESKEPQIRLGCNVSAPSGVLERVKEL-----MEDYSRAPSRQN 83
            ::.:|.:.|.|: ..::.|.|.:           ::|.|..|..|::|     :|.||....::.
Human   214 EMTIYIKQLGRRIFAREHGSNKK-----------LAAQSCALSLVRQLYHLGVVEAYSGLTKKKE 267

  Fly    84 VD-----DKNVDAKFQQQFRHLL-SVNFE--------------------EFVAETKERNADL--- 119
            .:     ..|:....:.|.:::: .:|.|                    :|....::....:
Human   268 GETVEPYKVNLSQDLEHQLQNIIQELNLEILPPPEDPSVPVALNIGKLAQFEPSQRQNQVGVVPW 332

  Fly   120 --------DWVNPKLD---------ERLQLELGQR---QLEE--NAKKRLEARKKLPTMKYADDI 162
                    .|.:..:|         |::.::|...   |||:  :.:..|:.|:.||..|:..:|
Human   333 SPPQSNWNPWTSSNIDEGPLAFATPEQISMDLKNELMYQLEQDHDLQAILQERELLPVKKFESEI 397

  Fly   163 IQAVRENQVILIVGSTGCGKTTQVPQILLDDAISRGCASSCRIICTQPRRISAIAIAEWVSYERC 227
            ::|:.:|.|::|.|:|||||||||||.:|||.|....|:.|.|:.||||||||:::||.|::||.
Human   398 LEAISQNSVVIIRGATGCGKTTQVPQFILDDFIQNDRAAECNIVVTQPRRISAVSVAERVAFERG 462

  Fly   228 ESLGNSVGYQIRLESRKARERASITYCTTGVLLQQLQSDPLMHNLSVLILDEIHERSVETDLLMG 292
            |..|.|.||.:|.||...|..|||.:||.||||::|::.  :..:|.:|:||||||.:.||.|:.
Human   463 EEPGKSCGYSVRFESILPRPHASIMFCTVGVLLRKLEAG--IRGISHVIVDEIHERDINTDFLLV 525

  Fly   293 LLKVILPHRPDLKVILMSATVREQDFCDYFNNCPMFRIEGVMFPVKMLYLEDVLSKTNYEFQKFR 357
            :|:.::...|:::::|||||:....||:||.|||:..:.|..:||:..:|||.:..|::.
Human   526 VLRDVVQAYPEVRIVLMSATIDTSMFCEYFFNCPIIEVYGRTYPVQEYFLEDCIQMTHFV----- 585

  Fly   358 DRRPKRDPPERRMKHEAMIEPYLRRIRNSYDSRVLDKLRLPESEGCEDIDFIADLVYYICENE-- 420
                   ||.:                   |.:..||    :.:|.||.|...:|   ||.:|
Human   586 -------PPPK-------------------DKKKKDK----DDDGGEDDDANCNL---ICGDEYG 617

  Fly   421 PE-------------------------------GAILVFLPGYDKISQLYNILDKPKTSKGQRWR 454
            ||                               ||:||||||::.|..:...|:........|::
Human   618 PETRLSMSQLNEKETPFELIEALLKYIETLNVPGAVLVFLPGWNLIYTMQKHLEMNPHFGSHRYQ 682

  Fly   455 DHMAVFPLHSLMQSGEQQAVFRRPPAGQRKVIISTIIAETSVTIDDVVYVINSGRTKATNYDIET 519
                :.||||.:...||:.||...|.|..|||:||.|||||:||:||||||:|.:.|...:....
Human   683 ----ILPLHSQIPREEQRKVFDPVPVGVTKVILSTNIAETSITINDVVYVIDSCKQKVKLFTAHN 743

  Fly   520 NIQSLDEVWVTKANTQQRRGRAGRVRPGICYNLFSRAREDRMDDIPTPEILRSKLESIILSLKLL 584
            |:.:...||.:|.|.:||:|||||||||.|::|.||||.:|::...|||:.|:.|..|.||:|||
Human   744 NMTNYATVWASKTNLEQRKGRAGRVRPGFCFHLCSRARFERLETHMTPEMFRTPLHEIALSIKLL 808

  Fly   585 HIDDPYRFLQTLINAPNPEAIKMGVELLKRIEALDQTGTLTPLGMHLAKLPIDPQMGKMILMSAL 649
            .:....:||...|..|..:|:......|:.::|||....|||||..||||||:|:.|||::|..:
Human   809 RLGGIGQFLAKAIEPPPLDAVIEAEHTLRELDALDANDELTPLGRILAKLPIEPRFGKMMIMGCI 873

  Fly   650 FCCLDPITSAAAALSFKSPFYSPLGKESRVDEIKRRMARNMRSDHLMVHNTIIAYRDSRY--SHA 712
            |...|.|.:.|||..|..||   :.:..|:..|.|..|.|..|||:.:.:...|:.|:|.  ..|
Human   874 FYVGDAICTIAAATCFPEPF---INEGKRLGYIHRNFAGNRFSDHVALLSVFQAWDDARMGGEEA 935

  Fly   713 ERDFCYKNFLSSMTLQQLERMKNQFSELLYNYKFLASSNCKDAASNKNSE-KIPLLRAIIGAGLY 776
            |..||....|:..||:.....|.|..|:|.|..|..........:|...: .:.::.:::..|:|
Human   936 EIRFCEHKRLNMATLRMTWEAKVQLKEILINSGFPEDCLLTQVFTNTGPDNNLDVVISLLAFGVY 1000

  Fly   777 PNMAHLRKSRQIKNRVRAIHTMATDDGRRVNFHPSSVN----SGESGFDSAYFVYFQRQKSTDLF 837
            ||:.:.::.|:|          .|.:||....|.||||    |.:..:.|.:||:.::.::..:.
Human  1001 PNVCYHKEKRKI----------LTTEGRNALIHKSSVNCPFSSQDMKYPSPFFVFGEKIRTRAIS 1055

  Fly   838 LLDSTMVFPMALIIFGDGVEAGVTQNTPYLCVAKTYYFKCNRETADVVIQLRSNLEKLLLKKALY 902
            ....|:|.|:.|::|   ....|..:...:.|......:.:.|.|..:..||:.:|.|:::....
Human  1056 AKGMTLVTPLQLLLF---ASKKVQSDGQIVLVDDWIKLQISHEAAACITGLRAAMEALVVEVTKQ 1117

  Fly   903 PAPIEE 908
            ||.|.:
Human  1118 PAIISQ 1123

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rhau	NP_610056.1	HrpA	160..>664	CDD:441249	209/536 (39%)
		OB_NTP_bind	767..856	CDD:400182	23/92 (25%)
DHX9	NP_001348.2	Interaction with CREBBP. /evidence=ECO:0000269|PubMed:9323138	1..250		9/46 (20%)
		DSRM_DHX9_rpt1	3..71	CDD:380683
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYY	5..9
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYY	53..55
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	83..118
		DSRM_DHX9_rpt2	178..252	CDD:380684	10/48 (21%)
		siRNA-binding. /evidence=ECO:0000269|PubMed:23361462, ECO:0007744|PDB:3VYX	182..186
		Interaction with BRCA1. /evidence=ECO:0000269|PubMed:9662397	230..325		15/105 (14%)
		siRNA-binding. /evidence=ECO:0000305|PubMed:23361462	234..236		1/1 (100%)
		Necessary for interaction with RNA polymerase II holoenzyme. /evidence=ECO:0000269|PubMed:9323138	255..664		131/448 (29%)
		Necessary for interaction with H2AX. /evidence=ECO:0000269|PubMed:15613478	313..952		243/685 (35%)
		DEXHc_DHX9	330..563	CDD:350730	93/234 (40%)
		MTAD. /evidence=ECO:0000269|PubMed:11416126	331..380		7/48 (15%)
		HrpA	388..>886	CDD:441249	210/541 (39%)
		Core helicase. /evidence=ECO:0000305|PubMed:25062910	398..809		176/454 (39%)
		DEIH box	511..514		2/2 (100%)
		Nuclear localization signal (NLS1). /evidence=ECO:0000255	586..595		3/27 (11%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	588..608		7/42 (17%)
		HA2. /evidence=ECO:0000269|PubMed:25062910	831..919		37/90 (41%)
		OB-fold. /evidence=ECO:0000269|PubMed:20696886, ECO:0000269|PubMed:25062910, ECO:0000269|PubMed:25149208	958..1074		29/128 (23%)
		OB_NTP_bind	994..1073	CDD:400182	23/91 (25%)
		RGG. /evidence=ECO:0000269|PubMed:10207077, ECO:0000269|PubMed:25062910, ECO:0000269|PubMed:25149208, ECO:0000269|PubMed:9111062	1150..1270
		Nuclear localization signal (NLS2). /evidence=ECO:0000269|PubMed:16375861	1155..1173
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1229..1248