DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ns4 and Gnl3

DIOPT Version :9

Sequence 1:	NP_610055.1	Gene:	Ns4 / 35338	FlyBaseID:	FBgn0032882	Length:	575	Species:	Drosophila melanogaster
Sequence 2:	NP_705775.2	Gene:	Gnl3 / 30877	MGIID:	1353651	Length:	538	Species:	Mus musculus

Alignment Length:	591	Identity:	130/591 - (21%)
Similarity:	227/591 - (38%)	Gaps:	147/591 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 RRKVAFSGKKKKDQMLQKRNTKGPPKYLRSTQESYEDSDVPETTRKLMEQPFARGGNRNKNVNRY 69
            ::||....:|.:.: .:||..|.|.|          |..||.:.      ||.....|...:.:.
Mouse    22 QKKVREHHRKLRKE-AKKRGHKKPRK----------DPGVPNSA------PFKEALLREAELRKQ 69

  Fly    70 NLQFYQEGKKELEQMKQEGFKPFEKLSPAQREVDDRYFAGCDFPVRPPWTLTESKEELDRTENRY 134
            .|:..::.:| |::.|::..|...::||...:.:                 .|::||.|..:
Mouse    70 QLEELKQQQK-LDRQKEQERKRKLEVSPGDEQSN-----------------VETREESDEPK--- 113

  Fly   135 FKEYVDELQKKQRAG--DSKELSLFELNLETWRQLWRVLEFSDILLIIVDVRYATLMFPPSLYDY 197
                    :||.:||  :.|:|...||.        :|:|.|||:|.::|.|.......|.:.:.
Mouse   114 --------RKKAKAGKQNPKKLHCQELK--------KVIEASDIVLEVLDARDPLGCRCPQIEEA 162

  Fly   198 IINTLKKHAIVVFNKVDLVEPHAVVAWRQYFRDRYPQLPVVLF-ASFLPRSRKGSQRGPQAHRRS 261
            :|.:..|..|:|.||.|||....:..|..|..   .:||.|:| ||...::||
Mouse   163 VIQSGSKKLILVLNKSDLVPKENLENWLNYLN---KELPTVVFKASTNLKNRK------------ 212

  Fly   262 MEGVYNIYKECQRYVQGEVDLTTWEQKI---REDMRSDQLDILDEISTAVEGELKISSSIDTTPH 323
               .:.|.|:         .:..::.||   :|.:            ..:.|:.:.|...|
Mouse   213 ---TFKIKKK---------KVVPFQSKICCGKEAL------------WKLLGDFQQSCGKD---- 249

  Fly   324 EHVKYHSGVLTIGCIGFPNVGKSSLINALKGRKVVSVSRTPGHTKHFQTIFLTPLVRLCDCPGLV 388
                     :.:|.||||||||||:||:||...:.:|..:.|.|:..|.:.|...:.:.|.|.|:
Mouse   250 ---------IQVGVIGFPNVGKSSVINSLKQEWICNVGISMGLTRSMQIVPLDKQITIIDSPCLI 305

  Fly   389 F-PSSTPKSLQVLLGSFPISQLAVPYRSLKFLGEHLNLPQLLRLHLPEDYDEWSAVAISDAWAYK 452
            . |.::|.:| .|.....|.:|.....:...|.:..|...:|:..:||..|   ::......|.:
Mouse   306 ISPCNSPTAL-ALRSPASIEELRPLEAASAILSQADNEQVVLKYTVPEYKD---SLHFFTKLAQR 366

  Fly   453 RGFLTAKAARPDRYRAANHILRMCLAGQQMLVLQFY--PPGFEERREHWLQHPDVGEVKKYQQVE 515
            || |..|...|:...||..:........    |.:|  ||.......|:.::......|.:...|
Mouse   367 RG-LHQKGGSPNVESAAKLVWSEWTGAS----LGYYCHPPASWNHSLHFNENIAAVMKKGFNLEE 426

  Fly   516 LDEPESE---------------------TNG--DTSSVCSDTADSEDEDEDSSNDETNADEEECG 557
            |::..:.                     |||  |...:..::....::.:|:.:.|..:.|....
Mouse   427 LEKNNAHSIQVLKGPHLTNRILFRSSGLTNGILDEKDIVEESPRQTEDKQDADDQENGSGERNAE 491

  Fly   558 IREDAP 563
            |.:.||
Mouse   492 ISDVAP 497

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ns4	NP_610055.1	HSR1_MMR1	163..390	CDD:206750	59/231 (26%)
Ns4	NP_610055.1	GTPase_YlqF	165..474	CDD:274669	79/313 (25%)
Gnl3	NP_705775.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..57		12/51 (24%)
		Basic. /evidence=ECO:0000250	2..46		7/24 (29%)
		GN3L_Grn1	16..93	CDD:285863	19/88 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	69..126		15/85 (18%)
		GTPase_YlqF	130..395	CDD:274669	81/333 (24%)
		Nucleostemin_like	140..302	CDD:206753	54/213 (25%)
		Intermediate. /evidence=ECO:0000250	277..451		36/182 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	460..538		7/38 (18%)
		Acidic. /evidence=ECO:0000250	460..532		7/38 (18%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG1161
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810