DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RtGEF and arhgef6

DIOPT Version :9

Sequence 1:	NP_001097184.2	Gene:	RtGEF / 35306	FlyBaseID:	FBgn0015803	Length:	1310	Species:	Drosophila melanogaster
Sequence 2:	XP_005173279.1	Gene:	arhgef6 / 558288	ZFINID:	ZDB-GENE-101013-2	Length:	795	Species:	Danio rerio

Alignment Length:	656	Identity:	223/656 - (33%)
Similarity:	332/656 - (50%)	Gaps:	118/656 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 LVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETI 69
            |||:|.::|..:|.|||.|.||::|.||::|:|||||||||.||||||||||.|.|   |..:.|
Zfish   161 LVVKARFNFKQNNEDELSFSKGELIHVTRQEEGGWWEGTLNSKTGWFPSNYVREVK---PCDKPI 222

  Fly    70 RP--------PEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNF 126
            .|        |:..:.|.|||::|:|:.||..|.|||.:|..:|.|:|.:..||..:.:.|..|.
Zfish   223 SPKAIKGFDVPQLTKNYYSVVVQDILEHEREFVKELQNVLGGYLRPLQASDKLSSVDCSSLNGNL 287

  Fly   127 VEIVRTHEDLLIQIEECN------DRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKDELEK 185
            .:|:...:.|.:.:|||:      .|:|..:|.....::.::.:||::||.|:.:|..:.:||:|
Zfish   288 EDILSFQQGLCLTLEECSKVPEGQQRIGGCYLNMMCQIRTLYLSYCSSHPSAVCVLTDHSEELDK 352

  Fly   186 YMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIAATCS 250
            :||.|||:.||:|.|||.|||||.|||||..:|||||||:|.:|||..|..::.|.:|::...|.
Zfish   353 FMESQGASAPGILTLTTSLSKPFMRLDKYPTLLQELERHVEEAHPDYSDILKATAAFKNLVTQCQ 417

  Fly   251 ATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSV----AVGADHRDRYFVLFPQTLLFLSVS 311
            ..|::|.||||:|:.|||||:|..:.:||.:|:|..:    :...:..:|||:|||..::.||.|
Zfish   418 DLRKRKNLELQILSEPVRGWEGDSMKSLGQVIYMSQIHMQSSANEEKEERYFMLFPNVIIMLSAS 482

  Fly   312 QRMSAFIYEGKLPLTGIIVNRL-EDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNANNP 375
            .|||.|||:|:|||||..|.:. ||.:....||||:...|||.:..|....|...|:|.|   :|
Zfish   483 PRMSGFIYQGRLPLTGTSVTKQPEDAETGHYAFEITGGSIDRTMVFCSSLQEQQDWLEHL---HP 544

  Fly   376 SLPMG--IKRQLSNLSNSSLGHLNAAHLSQHLDSRGYCTRFSLCAYYSSPPCHVRPLRVTLPPSN 438
            .:.:|  .:..|..::      ..||..|..|:|:.  ....||...:          |.:.|..
Zfish   545 YIKVGSPFETVLKVIA------FFAAFTSIQLNSKS--NVMFLCILQN----------VDVKPLT 591

  Fly   439 YPATAPYANLSAHFARLVKGGGLRSAIVKMLLYPQARQSIDLKRIALRKRRCHKASAKLKD---L 500
            .|.|:.: :|.......|..|.|..        |:..:...|.        |.:.:..||.   |
Zfish   592 MPGTSSH-HLHGFSTTAVNRGPLEP--------PKITKPWSLS--------CLRPAPPLKPSAAL 639

  Fly   501 NTNQDSGQSELERQDAIELP---TDSESYDDDF---------EDDFLHSCDSDPFEYVQFYQNKR 553
            ...:||.:|  .|.....||   |:.:..||:|         |:      |:...:.::.|
Zfish   640 GYKEDSSKS--PRPIKKFLPKRKTERKPSDDEFLLRKSTAALEE------DAQILKVIEAY---- 692

  Fly   554 NDSMCNSTGTFVDHGTGA-RRHCSS-----------INLIKLDSADT-------DEVLAL----N 595
                |  ||..:...|.| |:.|..           :..||.:...|       |.|.||    :
Zfish   693 ----C--TGASLHQSTTAVRKECVPQVLLPEEEKIIVEDIKSNGQTTIEEKSLVDAVYALKDEVH 751

  Fly   596 ELKKES 601
            |||||:
Zfish   752 ELKKEN 757

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810	34/51 (67%)
		RhoGEF	79..249	CDD:238091	71/175 (41%)
		PH_Cool_Pix	281..376	CDD:269932	38/99 (38%)
arhgef6	XP_005173279.1	CH	3..106	CDD:237981
		RhoGEF67_u1	115..157	CDD:293221
		SH3	160..216	CDD:302595	36/54 (67%)
		RhoGEF	243..418	CDD:279015	70/174 (40%)
		PH_Cool_Pix	448..547	CDD:269932	39/101 (39%)
		PH	448..547	CDD:278594	39/101 (39%)
		RhoGEF67_u2	604..704	CDD:293220	26/133 (20%)
		betaPIX_CC	706..792	CDD:293131	14/52 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	148	1.000	Domainoid score	I4413
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D251777at33208
OrthoFinder	1	1.000	-	-		FOG0002550
OrthoInspector	1	1.000	-	-		otm24330
orthoMCL	1	0.900	-	-		OOG6_103985
Panther	1	1.100	-	-	LDO	PTHR46026
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3079
SonicParanoid	1	1.000	-	-		X1675
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	10	9.950