DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RtGEF and arhgef7a

DIOPT Version :9

Sequence 1:	NP_001097184.2	Gene:	RtGEF / 35306	FlyBaseID:	FBgn0015803	Length:	1310	Species:	Drosophila melanogaster
Sequence 2:	XP_021334338.1	Gene:	arhgef7a / 553493	ZFINID:	ZDB-GENE-060322-11	Length:	864	Species:	Danio rerio

Alignment Length:	716	Identity:	229/716 - (31%)
Similarity:	355/716 - (49%)	Gaps:	133/716 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 LVVQAEYSFMGSNNDELCFQKGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQ----LPL 65
            |||:|.::|..:|.|||.|.|||:|:||:.|:||||||.||.||||||||||.|.|..    .|.
Zfish   171 LVVKARFNFQQTNEDELTFSKGDLISVTRTEEGGWWEGILNGKTGWFPSNYVKEVKGSDKQVSPK 235

  Fly    66 TETIRPPEE-------IQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLM 123
            :..::.|.:       .:.|.::||:::|::|..:..|||.||.::|.|:|.|:.||..:.:.::
Zfish   236 SGNLKSPPKGFDTASISKTYYNLVLQNILETETEYSRELQNLLTSYLRPLQMTERLSPADVSVIL 300

  Fly   124 CNFVEI-------VRTHEDLLIQIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKD 181
            .|..||       |::.|| |.::.|...:||..||...|.|:.::.:||:.||..:.:|..:.:
Zfish   301 GNLEEISTFQQTLVQSLED-LTKLPELQQKVGGFFLNLMPQMRSLNVSYCSNHPSTVNVLTVHSE 364

  Fly   182 ELEKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPDRGDTQRSVAVYKDIA 246
            ||.::||.:||:.||:|.||:||||||.|||:|..:|:|||||||..||||.:.|:.:..:|:::
Zfish   365 ELGEFMEARGASVPGILTLTSGLSKPFMRLDRYPTLLKELERHMEEGHPDRTEIQKCMTAFKNLS 429

  Fly   247 ATCSATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAV---GADHR-DRYFVLFPQTLLF 307
            |.|...|::||||||:|...:|.|:|:::.|||.:::|....|   |.:.: :||.:|||..||.
Zfish   430 AQCQEVRKRKELELQILAETIRLWEGEDIKTLGSVLYMSQAMVQNHGCEEKNERYLLLFPHVLLI 494

  Fly   308 LSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELLNA 372
            ||.|.|||.|||:|||||:|:.|..|||.::.|:|||:|..:.:|:..:|....:.:.|:|.||.
Zfish   495 LSASPRMSGFIYQGKLPLSGMTVTPLEDCESHKDAFELSGSMFERLQVICYNKQDLHDWLEHLNR 559

  Fly   373 NN--------------------PSLPMGIKRQLSNLSNSSLGHLNAAHLSQHLDSRG--YCTR-- 413
            ..                    ||.|:...|   :..:..|....|.|...|..|.|  :.|.
Zfish   560 QTKHTTMAAPSMKPLTVPCHTLPSHPLTPSR---HGESRGLTVAPAYHTLPHPSSHGTPHSTMMW 621

  Fly   414 -----------FSLCAYYSSPPCHVRP----------------LRVTLP---PSNYPATAPYANL 448
                       :||.....:||  :||                ::..||   |...|:...:|
Zfish   622 GPLEPPKTQKPWSLSCLRPAPP--LRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFA-- 682

  Fly   449 SAHFARLVKGGGLRSAIVKMLLYPQARQSIDLKRIALRKRRCHKASAKLKDLNTN---QDSGQSE 510
                        ||.:.|.:....|..:.|:....:.:.|:...::.:..||..|   ..|....
Zfish   683 ------------LRKSTVALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLDQSSADS 735

  Fly   511 LERQDAIELP--TDSESYDDDFEDDF-LHSC----------DSDPFEYVQFYQNKRNDSMCNSTG 562
            |.|:.:|..|  |...|.|.|::..: .||.          |..|...|...:....|.:.::..
Zfish   736 LGRRSSISRPEATSDLSEDSDYDSIWTAHSYRMGSVTRQWKDGSPALIVPGDEKFSVDELKSNGQ 800

  Fly   563 TFVDHGTGARRHCSSINLIKLDSADTDEVLALNELK------KESLVIGSRALRALAR--KSTTR 619
            |.::..          :|:.:..|..|||   .|||      |:||....||.:.|.|  |...:
Zfish   801 TLLEEK----------SLVDVVYALRDEV---QELKQDYKKMKKSLEEEQRARKDLERIVKKMLK 852

  Fly   620 N 620
            |
Zfish   853 N 853

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810	34/51 (67%)
		RhoGEF	79..249	CDD:238091	70/176 (40%)
		PH_Cool_Pix	281..376	CDD:269932	40/118 (34%)
arhgef7a	XP_021334338.1	CH	21..106	CDD:214479
		RhoGEF67_u1	116..168	CDD:318756
		SH3_alphaPIX	170..226	CDD:212993	36/54 (67%)
		RhoGEF	256..433	CDD:238091	70/177 (40%)
		PH_Cool_Pix	464..562	CDD:269932	40/97 (41%)
		RhoGEF67_u2	621..715	CDD:318755	17/109 (16%)
		betaPIX_CC	775..861	CDD:318676	22/92 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	148	1.000	Domainoid score	I4413
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D251777at33208
OrthoFinder	1	1.000	-	-		FOG0002550
OrthoInspector	1	1.000	-	-		otm24330
orthoMCL	1	0.900	-	-		OOG6_103985
Panther	1	1.100	-	-	O	PTHR46026
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3079
SonicParanoid	1	1.000	-	-		X1675
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	9	8.950