DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RtGEF and plekhg4

DIOPT Version :9

Sequence 1:	NP_001097184.2	Gene:	RtGEF / 35306	FlyBaseID:	FBgn0015803	Length:	1310	Species:	Drosophila melanogaster
Sequence 2:	XP_009301619.1	Gene:	plekhg4 / 326099	ZFINID:	ZDB-GENE-030131-4824	Length:	2630	Species:	Danio rerio

Alignment Length:	1558	Identity:	292/1558 - (18%)
Similarity:	515/1558 - (33%)	Gaps:	490/1558 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    53 SNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLD-SERAHVAELQGLLENFLEPMQQ-TQILS 115
            |..::.| :|..|: .:.||.|..  .:.||..:|| .|..:..:....:.:||.|.:. .|.:.
Zfish     3 SESLDSC-IQSTLS-ALYPPFEAT--AATVLCQVLDVVETTYRGDGLRYIIDFLVPAKHILQSIQ 63

  Fly   116 QD---EYAQLMCNFVEIVR-------THEDLLIQIEECNDRV---GKLFLTSAPLMK-----KVH 162
            ||   .|    |.|  :.|       .||.::||:...:.||   ...:|...|..|     ||
Zfish    64 QDACFPY----CGF--LFRHEGWPLCVHEKVIIQLSSLDWRVLRPSDFYLQVTPSAKSPPRIKV- 121

  Fly   163 QAYCAAHPKAIVILDKYKDEL---------------------EKYMERQGAATPGLLVLTTGLSK 206
                    |.:.:..::.:||                     |:.:.|:.|....||.....:.:
Zfish   122 --------KCLAVTGQHAEELDVPELAYTSLFTMDWLDSINQERSVVRRAALEHCLLSADNDIFR 178

  Fly   207 -PFRR------LDKYSAMLQELERHMESSHPDRGDTQRS------------VAVYKDIAATCSAT 252
             |:..      :.:.|.:.::.|....:.....||.:.|            ..::.|.:...|..
Zfish   179 VPWEDIVHPEFISRPSKVAEQSESRRVAKEVANGDGRDSDVEQDNNPEDIQCRIFYDTSTNHSGD 243

  Fly   253 RRQKELELQVLTGPVRGWQGQELSTLG-DIIHMGSVAVGADHRDRYFVLFPQTLLFL-----SVS 311
            ...:..|:::|  ||       ||.:. ||....|.:...|....|..|...:|...     |::
Zfish   244 MDCQSKEVKLL--PV-------LSEINKDISGRSSSSTADDSEGEYVELADISLPRFSPQKGSLT 299

  Fly   312 QRMS---AFIYEGKLPLTGIIVNRLEDTDALKN---------AFEISSPLID---RIVAVCQGPN 361
            |.:|   ..:::.:.|::..:..: .|...|||         |..|...|.|   .::.....|.
Zfish   300 QAISMNYRNLHKPQTPVSTQLKTK-PDESLLKNGACSQSMVCAMMIEESLSDTYQTVILTPTAPA 363

  Fly   362 EANKWVELLNANNPSLPMGI-----------KRQL----SNLSNSSLGHLNAAHLSQ-------- 403
            ||.:.::.::.:.|:...|.           :|:|    |:..::||..::..::||
Zfish   364 EAERPLQQVDMSEPACAAGTASFDSVSEHESERELLSQHSDCEDASLSDISTINISQTDIKPINT 428

  Fly   404 -HLDSRGYCTRFSLCAYYSS---------PPCHVRPLRVTLPPSNYPATA-PYANLSAHFARLVK 457
             .||:...|.......|.:|         .......|.::....|...|: |..|...
Zfish   429 SQLDNNVVCKSQDSEPYSTSQLDKNLVNTSQTDTESLSMSQLDDNLDCTSHPDVNQKP------- 486

  Fly   458 GGGLRSAIVKMLLYPQARQSIDLKRI--------ALRKRRCHKASAKLKDLNTNQDSGQSEL--- 511
                 |::.::.|.|.....:|.|.|        :|.......::...|.::|:|.....:|
Zfish   487 -----SSMSQLELEPLTVSQLDSKLISTSQPDSTSLDVEPVSTSNVDNKPVSTSQPDSTLQLVNE 546

  Fly   512 ----------------ERQDAIELPTDSESYDDDFEDDFLHS-----------CDSDPFEYVQFY 549
                            :.:....|..||:.......:.|..|           .||.|   |...
Zfish   547 PVKTSQDGEPVSRFHVDHKPVSTLQPDSQPVITSVNEPFRTSQPDNKSVSTTQLDSKP---VSVS 608

  Fly   550 QNKRNDSMCNSTGTFVDHGTGARRHCSSINLIKLDSADTDEVLALNELKKESL---VIGSR--AL 609
            |:.:.:|  .|..|.||:           ..:|....|| |.|.:::...:.:   .:||:  ::
Zfish   609 QHNKQES--KSVNTSVDN-----------EPVKTSQPDT-ESLRISQFDSKPVSNSQLGSKQVSI 659

  Fly   610 RALARK--STTR--NSSVHTSTATLELGVGG--------SITNCVEEEPILKVKPSFSLQQQSS- 661
            :.|..:  ||.:  |.||.|:....:|..|.        |:...|:.||:...:....|.:.|.
Zfish   660 KQLVNEIFSTPQPDNKSVSTTQLERKLVCGSQDNKQESKSVNTLVDSEPVKTSQTDTELLRISQF 724

  Fly   662 DASSIFAARLGGAFTACENLAS------MPDDLSRESSIQEPPTPLPASPTERHSMPTIFVGNRF 720
            |:..:..::|.|...:...|.:      .||:.|             .|||:..|.
Zfish   725 DSKPVSNSQLDGKQVSISQLVNEIFSTPQPDNKS-------------VSPTQLDSK--------- 767

  Fly   721 NHSKNTEVYVPTWRDRQEMQ--NQSVDAK-------QDEELHSSSIDLPAACLSAPDKLQAELLY 776
                    .|.|.:|:||.:  |.|:|.:       ..|.|..|..|......|..|..|..:..
Zfish   768 --------LVSTLQDKQESKSVNNSIDNEPVKTSQADTEPLKRSQFDSNPVSNSQLDGKQVSISQ 824

  Fly   777 NYDEILEKPLQLHREL--TPFPGHNLNSDKRVSHKSDSPSTGNAK---TDPNLATRSSSTTELCI 836
            ..:||...|...::.:  |...| |::...::..|.:|.|..::|   ..|:  .....|::..:
Zfish   825 LVNEIFSTPQPDNKSVSTTQLDG-NIDRTSQLDSKQESVSQTDSKPVSISPD--NEPVKTSQPQL 886

  Fly   837 DTT---SKKRTTPSERSRDSIRRCISYQFLQMSNRPPPPP-------PPRRDPDLHLDTKCRCCE 891
            ::|   :.|:.:.|:...:.|:          :::|...|       .....||.:.|.|
Zfish   887 ESTLHPNSKQASMSQLVNEHIQ----------TSQPDLKPVSIAQLYSEDAKPDGYADVK----- 936

  Fly   892 NSQCPSPRSSDSGMAGS--------------CT---ITSPD-PPNPESY---FPM---------- 925
                |.....|:|:..:              ||   ||.|: .||..||   .|:
Zfish   937 ----PITTELDNGLVSTSQPDNQPVDTPQAECTTVNITLPEGNPNDTSYIVNLPLSTSQPNSKTV 997

  Fly   926 --EAAGHDMLDNVEPE-RFDVCGMFREKFLTPEATQDVVDLSEEQPQLSDEPTTPTNRKEEPTCI 987
              |.|..::::..:|| ....|.:.||.  :.:.:...::|....|.            .|..|
Zfish   998 HVEQADINVINTSQPETEMLSCVLEREN--SEQVSPAYIELIVSDPY------------AEVRC- 1047

  Fly   988 TSAQVQVNTRSIFLPSSSSMDETNRNVPANNILFSSSSADQLEPQATFRSGMYAHWWKKERLPPE 1052
            .|.:::||...    ..|...:.||...|..:..|.||.:|::  .|..|....|  |....|.|
Zfish  1048 ESKELRVNCDL----EGSCGSQNNRASLAETVAESVSSQNQID--QTGNSLYQIH--KPIHTPSE 1104

  Fly  1053 VVRGI-------------AHAYNKS---LPSKDSKDSGSVCSSCFCSLGASGYSEGALYCSVCQN 1101
            .:..:             :|..|:|   |.....:|.|||.        |:.|||.....||
Zfish  1105 QMHDLPTIKEDHQTFSSSSHNTNESVGDLSPIAEEDGGSVT--------AALYSEHVALVSV--- 1158

  Fly  1102 CADYYNGSVTSTTNTTTTTSSASCPLCSEDE-GMIAP------------THDSSSLD----CPIC 1149
                           ||....:...|.|:.: |..:|            |.:..::.    .|.|
Zfish  1159 ---------------TTVPGESPVTLISQGQVGTESPEDRINQTEVELQTKEERAVQNVAGMPPC 1208

  Fly  1150 NGRIASGAEEGKQSEMDRRP---DVAAI--------------------------KPHTPTARRPS 1185
            .|  |...|:...||.:.:.   ||.|:                          :..:|......
Zfish  1209 KG--AEMVEKKSSSEAEIKTDENDVVAVCRQEEVETSQTGLVDATPKSDVQVQEEVSSPVETDEK 1271

  Fly  1186 ASHSQQHPGRQQQR------REPQPPGVSGHQLDVASQTEPVVQPPSQLQSVHAHSASTTSSAST 1244
            ...|.|..|.:::.      ||.:....|..:..|.|....|:.|.|.:.:.:.|    ..:..|
Zfish  1272 KQTSVQEKGVEEEEVKAKTVREQEAIVTSNSENRVQSACSEVLDPASDVPNTNGH----MHAQDT 1332

  Fly  1245 TTTKSAKSSGGIRPQIKFSPDTKQQDSSSTPGVIHGRQSSNSGSGSSSGGSGGNGGAKRKERK 1307
            .||:       ::.||     ||::|..   |....||.....|..       ||..|..:::
Zfish  1333 PTTE-------LKGQI-----TKEEDGK---GAEEKRQEDEIASSV-------NGSQKEHQQQ 1373

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810	1/4 (25%)
		RhoGEF	79..249	CDD:238091	41/229 (18%)
		PH_Cool_Pix	281..376	CDD:269932	21/114 (18%)
plekhg4	XP_009301619.1	COG5099	467..>912	CDD:227430	94/516 (18%)
		SEC14	1433..1573	CDD:238099
		BAR	<1707..>1807	CDD:299863
		RhoGEF	2130..2302	CDD:279015
		PH_puratrophin-1	2294..2430	CDD:270062
		PH	2319..2414	CDD:278594
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910