Protein Alignment RtGEF and Pura

DIOPT Version :10

• Sequence 1: NP_001097184.2
• Gene: RtGEF / 35306
• FlyBaseID: FBgn0015803
• Length: 1310
• Species: Drosophila melanogaster
• Sequence 2: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Alignment Length: 1229
• Identity: 228/1229 - (18%)
• Similarity: 372/1229 - (30%)
• Gaps: 483/1229 - (39%)

  Fly   307 FLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVEL-- 369
            |:||.| ::..:...:|| .|::.               |.|.:..:..:.|..::|.||.|.
  Fly   857 FISVDQ-VTTSVAPSQLP-AGLLP---------------SVPALSTLPGISQRHHDATKWREFFA 904

  Fly   370 -LNANNPSLPMGIKRQLSNLSN---SSLGHLNAAHLSQHLDSRGYCTRFSLCAYYSSPPCHVRPL 430
             |.|        .:||.|....   |:|..:.||      |.:|..||..|...:       |.|
  Fly   905 QLEA--------FQRQCSTAGGRLVSALSEIRAA------DLQGLPTRRQLYGQH-------RAL 948

  Fly   431 RVTLPPSNYPATAPYANLSAHFARLVKGGGLRSAIV------KMLLYPQARQS--------ID-- 479
            ...|..|...:.....  :...|||   ..|..||.      :|...||:..|        :|
  Fly   949 SRALMDSQLHSLRKMG--AGQLARL---QDLARAITSAPGSPRMTSQPQSSSSSSSGAFLNVDAA 1008

  Fly   480 --LKRIALRKRRCHKASAKLKDLNTNQDSGQSELERQDAIELPTDSESYDDDFEDDFLHSCDSDP 542
              |:::.|......:|:.:|:.|...:.....:|.||.|:|          |...:......||.
  Fly  1009 CKLRKVTLLFNEVDRAAQRLEQLTEQRRERLRQLTRQRALE----------DEISEVTSWMGSDG 1063

  Fly   543 FEYVQFYQNKRNDSMCNSTGTFVDHGTGARRHCSSINLIKLDSADTDE--VLALNELKKESLVIG 605
            .|.:|.:                     ::.|..:.:.::....:.::  .:|:..|.|
  Fly  1064 AENLQKF---------------------SQLHWDNESQLRTQEQEFEKYYFIAMKHLAK------ 1101

  Fly   606 SRALRALARKSTTRNSSVHTSTATLELGVGGSITNCVEEEPILKVKPSFSLQQQSSDASSIFAAR 670
            .|.|.|.|.|.:..:.|.:...:.|:                 :......|..:..||    |.|
  Fly  1102 GRDLHAAALKVSVLSESANNLKSALD-----------------QFAQKLELAHERYDA----AGR 1145

  Fly   671 LGGAFT-------ACENLASMPDDLSRESSIQEPPTP-------LPASPTERHSMPTIFVGNRFN 721
            |....|       |.|.|..:.:.|. .:|:.|...|       ||.:    .|.|....|.|.:
  Fly  1146 LLHLLTQHQRENAAREELQRLAEQLG-ATSLLEKHWPVMRKTHTLPVA----SSTPAAAAGKRVS 1205

  Fly   722 H-----SKNTEVYVPT------WR------------DRQEMQNQSVDAKQDEELHSSSIDLP--- 760
            :     |:::....|:      ||            :.:|.|:...|....||..|...|..
  Fly  1206 YRCSSASRSSFEGGPSSSNCSCWRESRNLEDMDQMEEEEEEQDHDCDGGDGEEQQSKIADSGLGV 1270

  Fly   761 -------------AACLSAPDKLQAELLYNYDEILEKPLQLHRELTPFPGHNLNSDKRVSHKSDS 812
                         .:|.|..:|...|||  .||..::|                 .||......|
  Fly  1271 CDNCERNPKLARICSCQSLNEKSHDELL--EDECFDRP-----------------SKRYMDIMHS 1316

  Fly   813 PSTGNAKTDPNLATRSSS-----TTEL-CIDTTSKKRTTPSER-----SRDSIRRCISYQFLQMS 866
            |...||    :|....||     ..|| |:|...:|......|     .||.:|..    :..:.
  Fly  1317 PMEANA----HLQCHGSSLELPKLEELSCLDPKIQKTLLLIMREMIGTERDYVRSL----YYVIE 1373

  Fly   867 NRPPPPPPPRRDPDLHLDTKCRCCENSQCPSP-RSSDSGMAGSCTITSPDPPNPESYFPMEAAGH 930
            |              ::|...|    ...|.| |...:.:.|          |.|..|..    |
  Fly  1374 N--------------YIDELLR----EDIPQPLRGQRNVIFG----------NIEKIFEF----H 1406

  Fly   931 DMLDNVEPERFDVCGMFREKFLTPEATQDVVDLSEEQPQLSDEPTTPTNRKEEPTCITSAQVQVN 995
            :.....|.||::                                       ..|..:.:|.:::.
  Fly  1407 NSHFLGELERYE---------------------------------------RNPLKVGAAFLEME 1432

  Fly   996 TR----SIFLPSSSSMDETNRNVPANNILFSSSSADQLEPQ----------ATF------RSGMY 1040
            ::    :::          |:|.|.::.|.|...:...:|:          |::      |.|.|
  Fly  1433 SKFYLYALY----------NKNKPKSDTLLSEYGSSFFKPKQMQLQDKLDLASYLLKPVQRMGKY 1487

  Fly  1041 AHWWKKERLPPEVV---RGIAHA------------------------YNKSLPSKDS-------- 1070
            |      .|..::|   :|:..|                        :...|.:.||
  Fly  1488 A------LLLQQLVKACKGVEGAALQEIAADVEELQRAEEMVKFQLRHGNDLLAMDSLRDCDVNV 1546

  Fly  1071 KDSGSVC--SSCFCSLGASG---------YSEGALYCSVCQNCADYYNGSVTSTTNTTTTTSSAS 1124
            |:.|.:.  :......|..|         :.|..|: |..:...|:.|..:....|:..|:
  Fly  1547 KEQGRLLRQNEFLVWQGRGGKKTLRQVFLFEELVLF-SKARRFPDHKNLDIYIYKNSIKTS---- 1606

  Fly  1125 CPLCSEDEGMIAPTHDSSS----------------LDC---PICNG------------------- 1151
                  |.|:.|.|.||::                |.|   .|.|.
  Fly  1607 ------DIGLTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWKQAKRNREV 1665

  Fly  1152 RIASGAEEGKQSE--MDRRPDVAAIKPHT-PTA---RRPSASHSQQHPGRQQQRREPQPPGVSGH 1210
            |:|..:..|..|:  :|.||....|...: |.|   :.|...||:  ||:...||
  Fly  1666 RLAEMSSMGIGSKPCLDIRPSNNQISDRSIPLAQLNKTPKLRHSE--PGKGSMRR---------- 1718

  Fly  1211 QLDVASQTEPVVQPPSQLQSVHAHSASTTSSASTTT--TKSAKSSGGIRPQIKFSPDTKQQDSSS 1273
                         |.|.:......|.::|:|.|:.:  :.|..:.||.|..:.|........:||
  Fly  1719 -------------PNSLISESSLSSGTSTTSGSSISGGSSSGHNDGGGRHSLHFGKLPGAHSASS 1770

  Fly  1274 T----------------------PGVIHGRQSSNSGSGSSSGGSGGNGGAKR-KERKHK 1309
            |                      .|.|.|..::..| |::||...|||.|.| .:|.||
  Fly  1771 TNCSATLELINETQALKLAKSPSGGGIKGADTAADG-GNTSGNGNGNGNAFRSSKRNHK 1828

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810
		RhoGEF	79..249	CDD:238091
		PH_Cool_Pix	281..376	CDD:269932	16/71 (23%)
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	34/204 (17%)
		RhoGEF	1353..1524	CDD:459876	35/261 (13%)
		PH_puratrophin-1	1524..1663	CDD:270062	24/149 (16%)