DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RtGEF and MCF2L2

DIOPT Version :9

Sequence 1:	NP_001097184.2	Gene:	RtGEF / 35306	FlyBaseID:	FBgn0015803	Length:	1310	Species:	Drosophila melanogaster
Sequence 2:	NP_055893.4	Gene:	MCF2L2 / 23101	HGNCID:	30319	Length:	1114	Species:	Homo sapiens

Alignment Length:	673	Identity:	124/673 - (18%)
Similarity:	236/673 - (35%)	Gaps:	191/673 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 QAEYSFMGSNNDELCFQ-KGDVITVTQREDGGWWEGTLNDKTGWFPSNYVNECKVQLPLTETIRP 71
            :.|.:..|..:||..|: |.:.|..:..|.|.                  .|.:.|..|.: :.|
Human   574 ETELNSRGKEDDETKFEVKSEEIFESHHERGN------------------PELEQQARLGD-LSP 619

  Fly    72 PEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDEYAQLMCNFVEIVRTHEDL 136
                   |..:::|||::|..::.|::.:::.::.||         ::..|.....::::.::|.
Human   620 -------RRRIIRDLLETEEIYIKEIKSIIDGYITPM---------DFIWLKHLIPDVLQNNKDF 668

  Fly   137 LI-QIEECNDRVGKLFLTSAPLMKKVHQAYCAAHPKAIV-----------ILDKYKDELEK---- 185
            |. .|.|..:...:.||      |::.:  ||.:|:.:.           |..||...|.:
Human   669 LFGNIRELYEFHNRTFL------KELEK--CAENPELLAHCFLKRKEDLQIYFKYHKNLPRARAI 725

  Fly   186 YMERQGAATPGL--------LVLTTGLSKPFRRLDKYSAMLQ-----ELERHMESSHPDRG---- 233
            :.|.|..|..|:        |.|...|..|.:||.||..:|:     |....||....:.|
Human   726 WQECQDCAYFGVCQRQLDHNLPLFKYLKGPSQRLIKYQMLLKGLLDFESPEDMEIDPGELGGSAK 790

  Fly   234 -------------DTQRSVAVYKDIAATCSATRRQKELELQVLTG-PVRGWQGQELSTLGDIIHM 284
                         :.|:::||.:|:..:|     :..::|..:|. |      .::..||.::..
Human   791 DGPKRTKDSAFSTELQQALAVIEDLIKSC-----ELAVDLAAVTECP------DDIGKLGKLLLH 844

  Fly   285 GSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNAFEISSPL 349
            |..:|...|:|||.:   :.|:....||| ..:::|..:....|   |:|..|
Human   845 GPFSVWTIHKDRYKM---KDLIRFKPSQR-QIYLFERGIVFCKI---RMEPGD------------ 890

  Fly   350 IDRIVAVCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHLSQHLDSRGYCTRF 414
                    ||             .:|........:|..||...||  ..:|....:.||....::
Human   891 --------QG-------------LSPHYSFKKTMKLMTLSIRQLG--RGSHRKFEIASRNGLEKY 932

  Fly   415 SLCA--------YYSSPPCHVRPLRVTLPPSNYPATAPYANLSAHFARLVKGGGLRSAIVKMLLY 471
            .|.|        ::|    .:..|.:....:......|...:|..     ||.|..|.
Human   933 ILQAASKEIRDCWFS----EISKLLMEQQNNIKDQGNPQFEMSTS-----KGSGAGSG------- 981

  Fly   472 PQARQSIDLKRIALRKRRCHKASAKLKDLNTNQDSGQSELERQDAIELPTDSESYDDDFEDDFLH 536
            |..:   :::|....|.....::..:|..::.:.|.....|         |.|..:|       .
Human   982 PWIK---NMERATTSKEDPASSTGGIKGCSSREFSSMDTFE---------DCEGAED-------M 1027

  Fly   537 SCDSDPFEYVQFYQNKRNDSMCNSTGTFVDHGTGARRHCSSINLIKLDSADTDEVLA---LNELK 598
            ..:|........:|:..:...|:|...|::.|..::......:..:..:..|:|..|   ...|.
Human  1028 EKESSALSLAGLFQSDDSHETCSSKSAFLERGESSQGEKEERDEEETATRSTEEERAGASTGRLA 1092

  Fly   599 KESLVIGSRALRALARKSTTRNS 621
            ......|.:| |||..:::.:.|
Human  1093 PAGATAGFQA-RALRPRTSAQES 1114

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810	9/50 (18%)
		RhoGEF	79..249	CDD:238091	44/215 (20%)
		PH_Cool_Pix	281..376	CDD:269932	17/94 (18%)
MCF2L2	NP_055893.4	SEC14	52..190	CDD:214706
		SPEC	321..523	CDD:238103
		Spectrin	323..428
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	530..614		11/57 (19%)
		RhoGEF	623..820	CDD:214619	44/218 (20%)
		PH-like	827..956	CDD:418428	34/180 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	962..1114		30/183 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910