DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RtGEF and Mcf2l

DIOPT Version :9

Sequence 1:	NP_001097184.2	Gene:	RtGEF / 35306	FlyBaseID:	FBgn0015803	Length:	1310	Species:	Drosophila melanogaster
Sequence 2:	XP_006253478.1	Gene:	Mcf2l / 117020	RGDID:	619782	Length:	1280	Species:	Rattus norvegicus

Alignment Length:	698	Identity:	151/698 - (21%)
Similarity:	243/698 - (34%)	Gaps:	189/698 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    73 EEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQ---QTQILS---QDEYAQLMCNFVEIVR 131
            |.:...|..|:.:|||:|||:|.||..:||.:...|.   ...::|   |::...|..|..||..
  Rat   696 ESLAILRRHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYH 760

  Fly   132 THEDLLI-QIEECND---RVGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKD-----ELEKYM 187
            .|..:.: ::|.|.|   .||:.||...... ::::.||...|::..:..:..|     |.:|.:
  Rat   761 FHNRIFLRELESCIDCPELVGRCFLERMEEF-QIYEKYCQNKPRSESLWRQCSDCPFFQECQKKL 824

  Fly   188 ERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQEL---ERHMESSHPDRGDTQRSVAVYKDIAATC 249
            :.:       |.|.:.|.||.:|:.||..:|:|:   .:|.|.:.    |.|.:::....|....
  Rat   825 DHK-------LSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAE----DLQEALSSILGILKAV 878

  Fly   250 SATRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHR---------------DRYFV 299
            :     ..:.|..:|    |:.| .|..||.::..||.:|..||:               .|:..
  Rat   879 N-----DSMHLIAIT----GYDG-NLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLF 933

  Fly   300 LFPQTLLFLSVS-------QRMSAFIYEGKLPLT--GIIVNRLEDTDALKNAFEISSPLIDRIVA 355
            |..:.:||....       ::..::.|:..|.:|  ||..|...||    ..|||.
  Rat   934 LHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDT----KKFEIW--------- 985

  Fly   356 VCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHLSQHLDSRGYCTRFSLCAYY 420
                 ..|.:.|.::.|..|.:......::..:..|.|.....|  |||               .
  Rat   986 -----YNAREEVYIIQAPTPEIKAAWVNEIRKVLTSQLQACREA--SQH---------------R 1028

  Fly   421 SSPPCHVRPLRVTLPPSNYPATAPYANLSAHFARLVKGGGLRSAIVKMLLYPQARQSIDL----- 480
            :....|..||  ..|.|..|......|:.....|......|...:...|..|..:...|.
  Rat  1029 ALEQSHSLPL--PTPASTSPTKGSTRNVKKLEDRKTDPLCLEGCVSSSLPKPPEKGKDDAVPSST 1091

  Fly   481 -KRIAL-RKRRCHKASAKLKDLNTNQDSGQSELERQDAIELPT---------DSESYDDDFEDDF 534
             :..|| |||...:..|.||....:..|...:.:|.:....||         .|.|.:...||..
  Rat  1092 SESSALSRKRFTLQGLANLKGQKASPTSPDKKAKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDGG 1156

  Fly   535 LHSCDSDPFEYVQFYQNKRNDSMCNSTGTFVDHGTGARRHCSSINLIKLD----SADTDEVLALN 595
            ..|.                :.:.||:....|.|.|.|:.......:.:|    .:||
  Rat  1157 WSSA----------------EELINSSDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDT------- 1198

  Fly   596 ELKKESLVIGSRALRALARKSTTRNSSVHTSTATLELGVGGSITNCVEE--EPILKVKPSFSLQQ 658
                            ||.:|                   |.:...|||  |.:..|:...|.::
  Rat  1199 ----------------LAMRS-------------------GDMVEVVEEGTEGLWYVRDLTSSKE 1228

  Fly   659 QSSDASSIFAARLGGAFTACENLASMPDD-----LSRESSIQEP-PTP 700
            ....|||:  |.|.|..::.:.|:|....     ||..||..|. |||
  Rat  1229 GWVPASSL--ATLLGKSSSAQCLSSSDSSPGSAVLSNSSSCSEGYPTP 1274

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810
		RhoGEF	79..249	CDD:238091	49/187 (26%)
		PH_Cool_Pix	281..376	CDD:269932	23/118 (19%)
Mcf2l	XP_006253478.1	SEC14	145..290	CDD:214706
		SPEC	421..623	CDD:238103
		RhoGEF	702..879	CDD:238091	49/188 (26%)
		PH_Dbs	886..1017	CDD:269934	30/153 (20%)
		PH	900..1014	CDD:278594	24/131 (18%)
		SH3_DBS	1184..1236	CDD:212791	15/93 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910