Protein Alignment RtGEF and plekhn1

DIOPT Version :10

• Sequence 1: NP_001097184.2
• Gene: RtGEF / 35306
• FlyBaseID: FBgn0015803
• Length: 1310
• Species: Drosophila melanogaster
• Sequence 2: XP_002936429.2
• Gene: plekhn1 / 100491834
• XenbaseID: XB-GENE-1004111
• Length: 724
• Species: Xenopus tropicalis
• Alignment Length: 754
• Identity: 149/754 - (19%)
• Similarity: 270/754 - (35%)
• Gaps: 222/754 - (29%)

  Fly    39 WWEGTLND-KTGWFPSNYV--NECKV-QLPLTETIR---PPEEIQEYRSVVLKDLLDSERAHVAE 96
            |.:|...| :|.|   .|:  ||..| :..:::.|.   |.::|..:.  :.||.:|
 Frog    24 WQQGKEQDGRTKW---TYLFGNESGVGRERISDNILHYIPGKDIGNHE--IQKDSVD-------- 75

  Fly    97 LQGLLENFLEPMQQTQILSQDE---YAQLM-----------CNFVEIVRTHEDLLIQIEECNDRV 147
             |..|..|.:..::|.:.:..:   |:::.           | ::|:.::|
 Frog    76 -QRFLSIFRKGKKKTIVRNMGQMIHYSKVKFCFQNSQDISDC-YLELFQSH-------------- 124

  Fly   148 GKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYK---DELEKYMERQGAATPGLLVLTTGLSKPFR 209
              |:..|.......:|........::..|::.|   :::::|..|          :|..|..|
 Frog   125 --LYFQSRGPTGLTYQGLVPLKELSVYKLERSKGPANQVQEYAFR----------ITGPLLNP-- 175

  Fly   210 RLDKYSAMLQELER---HMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQ 271
             |..|....|||::   |:|......|.   ::.|......:......:.||...|.:.||:.|:
 Frog   176 -LIVYCESQQELQKWLYHLEKQIQLNGG---NLDVISATEGSNDCNWEKSELRRSVQSQPVQDWE 236

  Fly   272 GQELSTLGDIIHMGSVAVGA----DHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGI-IVN 331
            ..:..:||.|..:..|.:..    :..:|..||:|.:|:.||...:...|  :|:|||..| :|:
 Frog   237 ETQRESLGSITCISKVKLQTLPFKELNERLLVLYPSSLVILSEEGKHLCF--KGELPLKAIRVVS 299

  Fly   332 RLEDTDALKNAFEISSPLIDRIVAVCQGPNEANKWVELL----NANNPSLPMGI----------K 382
              ::.:..:.:|.|....|:.|..:|....:..:|:|.|    ..|..|...|.          :
 Frog   300 --DEAEKKRKSFLIEGRYINTIRVMCLSQMDYEEWIEHLKRTQQQNRDSSLSGSSSFCGSHSAQQ 362

  Fly   383 RQLSNLSNSSL---GHLNA-------------AHLSQHLDSRGYCTRFS--------LCAYYSSP 423
            .||:....||.   ||..:             :|.:.|...|...|..:        |...||.|
 Frog   363 EQLTTSCRSSFTSDGHSKSWASGGKLPNSNRQSHSTTHGSDRQSVTVLAEHPMQDDPLSPGYSEP 427

  Fly   424 -PCHVRPLRVTLPPSNYPATAPYANLSAHFARLVKGGGLRS----AIVKMLLYPQARQSIDLKRI 483
             .|...|:              :::.:...:.|.:||..|.    :.:|:.:.|...||.||:..
 Frog   428 VHCFGNPV--------------WSSATNGLSDLQRGGNTRGSKGRSNMKVQIPPVLPQSYDLETT 478

  Fly   484 AL-----------------RKRRCHKASAK---------LKDLNTNQDSGQSELERQDAIELPTD 522
            |.                 |:..|::.|.|         |...:...||.||.....:.::....
 Frog   479 AFGLIPETPSEEEVMSPVYREPYCYEKSQKHSSGDHHNELNRWSLQSDSQQSYHSDSNNVKSSLY 543

  Fly   523 SESY------DDDFEDDFLHSCDSDPFEYVQFYQNKRNDSMCNSTGTFVDHGTGARRHCSSINLI 581
            :|.|      :.:|.|:.|.:.:|.        |.::..|...::|:|
 Frog   544 AEPYTPIDGLESNFMDEILKNLNSS--------QTEKPASKLKTSGSF----------------- 583

  Fly   582 KLDSADTDEVLALNELKKESLVIGSRAL-RALARKSTTRNSSVHTSTATLELGVGGSITNCVEEE 645
               ....:...||.: |..:.|.|::.| |.|..:::..::||||......||......:.|:||
 Frog   584 ---QISRNPHPALQK-KYSNPVKGNKQLVRPLMHRNSEPDTSVHTRAIPPALGPFFKSVSAVKEE 644

  Fly   646 PILKVK-----------PSFS---------LQQQSSDAS 664
            ..|..:           |.||         ||...||.|
 Frog   645 MALSYRNKAVEDMFDLPPPFSESLEHDYAELQSFHSDLS 683

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810	6/21 (29%)
		RhoGEF	79..249	CDD:238091	30/189 (16%)
		PH_Cool_Pix	281..376	CDD:269932	25/103 (24%)
plekhn1	XP_002936429.2	PH-like	98..197	CDD:473070	21/128 (16%)
		PH-like	240..345	CDD:473070	27/108 (25%)