DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RtGEF and tiam1b

DIOPT Version :9

Sequence 1:	NP_001097184.2	Gene:	RtGEF / 35306	FlyBaseID:	FBgn0015803	Length:	1310	Species:	Drosophila melanogaster
Sequence 2:	XP_009290217.1	Gene:	tiam1b / 100330358	ZFINID:	ZDB-GENE-120221-1	Length:	1562	Species:	Danio rerio

Alignment Length:	665	Identity:	151/665 - (22%)
Similarity:	247/665 - (37%)	Gaps:	192/665 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    53 SNYVNECKVQLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQD 117
            |:.|.|.....|.|.|.|...:..:.|.|:. :|:::||.:|.:|..|:..:|.|:|:...|:||
Zfish   972 SSSVPESSSLPPSTATPRQLSDADKLRKVIC-ELVETERTYVKDLNCLIGRYLTPLQKESFLTQD 1035

  Fly   118 EYAQLMCNF-------VEIVRTHED---------LLIQIEECND---RVGKLFLTSAPLMKKVHQ 163
            |...|..|.       ||.::|.||         .|.::||...   .:|..||..|... |::.
Zfish  1036 ELDVLFGNLPEMLEFQVEFLKTLEDGTRLVPDLEKLERVEEFKKILFSLGGSFLYYADRF-KIYS 1099

  Fly   164 AYCAAHPKAIVILDKYKDEL-------EKYMERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQEL 221
            |:||:|.|...:|.|.|.:.       |:..::|.::|     |.:.|.||.:|:.||..:|:||
Zfish  1100 AFCASHTKVPKVLVKAKTDAAFKAFLDERNPKQQHSST-----LESYLIKPIQRVLKYPLLLREL 1159

  Fly   222 --------ERH-------------------MESSHPDRGDTQRSVAVYKDIAATCSATRRQKELE 259
                    |.|                   |:..|.:.|      ||: |:..: ..|..:||:
Zfish  1160 HSLTDPDSEEHYHLNVAMKAMNTVASHINEMQKIHEEFG------AVF-DLLIS-EQTGEKKEV- 1215

  Fly   260 LQVLTGPVRGWQGQELSTLGDIIHMGSV-------AVGADHRDRYFV--LFPQTLLFL------- 308
                         .:|| :||::...||       ::|...::....  :|...::|:
Zfish  1216 -------------ADLS-MGDLLLHSSVTWINPSSSLGKCKKEPQLATFVFKTAVVFVCKDYSKQ 1266

  Fly   309 ----------SVS-QRMSAFIYEGKLPLTGIIVNRLEDTDALKNAF-EI------SSPLIDRIVA 355
                      ||| :....|.:...:|...:.|..|.:||....|. ||      |....:|:..
Zfish  1267 KKKMGGSHRASVSGEEKDPFRFRHMIPTDALQVRTLSNTDGESAAVCEIVHIKSESEGRPERVFQ 1331

  Fly   356 VCQGPNEANKWVELLNANNPSLPMGIKRQLSNLSNSSLGHLNAAHLSQHLDSRGYCTRFSLCAYY 420
            :|....|:.|  :.|...:..|....:|||  |...||      .|||.....|   ...|||
Zfish  1332 LCCSSPESKK--DFLKTVHSILRDKQRRQL--LKTESL------PLSQQYVPFG---GKRLCA-- 1381

  Fly   421 SSPPCHVRPLRVTLPPSNYPATAPYANLSAHFARLVKGGGLRSAIVKMLLY-----------PQA 474
                     |:...|..|...:||...|...  :||:.   |..|...:::           ..|
Zfish  1382 ---------LKGARPAMNRAVSAPSRTLGRR--KLVRN---RFTIDTDIVFDTEPDSDSPDSQHA 1432

  Fly   475 RQSIDLKRIALRKRRCHKASAKLKDLNTNQDSGQSELERQDAIELPTDSESYDDDFEDDFLH--- 536
            :|:|.:           .|....:.:....|..:.|.:.||..|  ||..|.|:||....:.
Zfish  1433 QQTIGV-----------PAGDTDRWVEEQFDLQRYEEQEQDVKE--TDILSDDEDFCPSAMSPST 1484

  Fly   537 -------SCDSDPFEYVQFYQNKRNDSMCNST-GTFVDHGTGARRHCSSINLIKLDSADTDEVL- 592
                   |.:.|..|..:..:.:.:...|.|. ....|.|.......:|::    :.|.:|:|:
Zfish  1485 EPLLSTLSLEGDTAEEEEEAEEEDSRKDCKSQHAKLSDVGKQCAMSVASVD----EQATSDDVVW 1545

  Fly   593 ------ALNELKKES 601
                  |.||.:.||
Zfish  1546 VRRDSGAENETQDES 1560

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810	2/4 (50%)
		RhoGEF	79..249	CDD:238091	59/222 (27%)
		PH_Cool_Pix	281..376	CDD:269932	23/128 (18%)
tiam1b	XP_009290217.1	PH1_Tiam1_2	387..513	CDD:269937
		PH	400..502	CDD:278594
		UBQ	721..796	CDD:294102
		PDZ_signaling	799..879	CDD:238492
		RhoGEF	1000..1189	CDD:214619	52/195 (27%)
		PH2_Tiam1_2	1191..1363	CDD:269957	40/198 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910