DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RtGEF and mcf2l2

DIOPT Version :9

Sequence 1:	NP_001097184.2	Gene:	RtGEF / 35306	FlyBaseID:	FBgn0015803	Length:	1310	Species:	Drosophila melanogaster
Sequence 2:	XP_021335410.1	Gene:	mcf2l2 / 100149630	ZFINID:	ZDB-GENE-100812-1	Length:	1284	Species:	Danio rerio

Alignment Length:	676	Identity:	136/676 - (20%)
Similarity:	261/676 - (38%)	Gaps:	143/676 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    73 EEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQIL------SQDEYAQLMCNFVEIVR 131
            |.:...||.::.:|:::||.:|.|||.::|.:...:...:::      .:::...|..|..||.:
Zfish   643 ESLSNRRSHIMNELIETERLYVEELQSIIEGYAAALDDPELIYLIPPSLENKKEVLFGNLPEIYQ 707

  Fly   132 THEDLLI-QIEECNDR---VGKLFLTSAPLMKKVHQAYCAAHPKAIVILDKYKD-----ELEKYM 187
            .|:.:.: ::|...::   ||..||.....: ::::.||...|::..:..:..|     |.:|.:
Zfish   708 FHQKIFLKELENSAEKPELVGTCFLKRKEEL-QIYEKYCQNKPRSEALWRQCGDSMFFQECQKRL 771

  Fly   188 ERQGAATPGLLVLTTGLSKPFRRLDKYSAMLQELERHMESSHPD-RGDTQRSVAVYKDIAATCSA 251
            :.:       |.|...|.||.:|:.||..||:|:.:.  |.:|: ..:.:.:.|...||..:.:.
Zfish   772 DHK-------LSLDAYLLKPVQRITKYQLMLKEMLKC--SKNPEGTAELEEAQATVLDIIKSVND 827

  Fly   252 TRRQKELELQVLTGPVRGWQGQELSTLGDIIHMGSVAVGADHR---------------DRYFVLF 301
            :..|.         .:.|::| .|:.||.::..||..|..||:               .|:..|:
Zfish   828 SMHQI---------AITGFEG-SLNDLGKLLMQGSFNVWTDHKKGHSKVKDLARFKPMQRHLFLY 882

  Fly   302 PQTLLFLSVSQRMS---------AFIYEGKLPLTGIIVNRLEDT-------DALKNAFEISSPLI 350
            .:.:||....:.|:         :|.:..|:...||..|...|.       :..:..:.|.:|.:
Zfish   883 HKMILFCKKREEMTDGHEKSPSYSFKHSLKMGSVGITENVKGDIKKFEIWYNGREEVYIIQAPSM 947

  Fly   351 D---------RIVAVCQ--GPNEANKWVELLNANNPSLPM------------------GIKRQLS 386
            |         |.|...|  ...||::..:.:..|....||                  |.:|..|
Zfish   948 DVKNTWVSEIRKVLTSQLEACREASQLHQKITENIYHAPMRNMRKTALRQSDSSSPETGYRRSDS 1012

  Fly   387 --NL------SNSSLGHLNAAHLSQHLDSRGYCTRFSLCAYYSSPPCHVRPLRVTLPPSNYPATA 443
              |:      ::||||...||...:....:|...|.||.|..:.....| ..|.:|..:...|||
Zfish  1013 GPNMRQKRADTSSSLGSERAALPRKRFTLQGLSNRRSLPAEPAPKETEV-TRRFSLASTVGSATA 1076

  Fly   444 PYANLSAHFARLVKGGGLRSAIVKMLLYPQARQSIDLKRIALR----KRRCHKASAKLKDL--NT 502
            |........:...|...::|.... ..|..|.::...|:..:|    ..|..|.|...|..  .:
Zfish  1077 PRTKAPLSSSLKSKRHQIKSDPTP-FGYEGATKTRGFKKETMRDSLPAERKSKGSGGSKPAVPRS 1140

  Fly   503 NQDSGQSE-----LERQDAIELPTDSE--SYDDDFEDD-FLHSCDSDPFEYVQFYQNKRNDSMCN 559
            ....|.|:     ::.:|...:|:.:|  |...|.||: ...:.|:|.::....|::.....:..
Zfish  1141 ASQPGWSQRRLPSMDAEDFETIPSSAEELSISSDGEDEGHNKNGDNDRYQVQLTYESCTAQDLSV 1205

  Fly   560 STGTFVDHGTGARRHCSSINLIKLDSADTDEVLALNELKKESLVIGSRALRALARK-STTRNSSV 623
            ..|.||..               |:.::....|..|...:::.::....|::.|.: ....||.:
Zfish  1206 EAGDFVQF---------------LEESENGLWLVKNLRTQKTGLVPPTVLQSTAEELDLYSNSDI 1255

  Fly   624 HTSTATLEL-----GVGGSITNCVEE 644
            .:...|..|     .:||  ||..::
Zfish  1256 FSDLCTAALTDTEDDIGG--TNPAQD 1279

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810
		RhoGEF	79..249	CDD:238091	42/185 (23%)
		PH_Cool_Pix	281..376	CDD:269932	25/136 (18%)
mcf2l2	XP_021335410.1	SEC14	77..217	CDD:214706
		SPEC	348..554	CDD:238103
		RhoGEF	649..826	CDD:238091	42/186 (23%)
		PH_Dbs	833..964	CDD:269934	26/131 (20%)
		Mating_C	932..>1122	CDD:315419	39/191 (20%)
		SH3_DBS	1188..1242	CDD:212791	8/68 (12%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910