Protein Alignment RtGEF and plekhg4b

DIOPT Version :10

• Sequence 1: NP_001097184.2
• Gene: RtGEF / 35306
• FlyBaseID: FBgn0015803
• Length: 1310
• Species: Drosophila melanogaster
• Sequence 2: XP_073780503.1
• Gene: plekhg4b / 100009641
• ZFINID: ZDB-GENE-101112-5
• Length: 1612
• Species: Danio rerio
• Alignment Length: 1235
• Identity: 248/1235 - (20%)
• Similarity: 407/1235 - (32%)
• Gaps: 396/1235 - (32%)

  Fly    62 QLPLTETIRPPEEIQEYRSVVLKDLLDSERAHVAELQGLLENFLEPMQQTQILSQDE--YAQLMC 124
            ::|::|::        |.||...|.|.:...         :..|:|:|.. :||...  |.....
Zfish   153 EVPVSESM--------YGSVFTPDFLQNVTR---------DRNLQPLQNC-LLSTGSVVYRTPWK 199

  Fly   125 NFVE--IVRTHEDLLI------------QIEECN-----------DRVGKLFLTSAPLMKKVHQA 164
            |.:.  :|.:.||.|:            ||..|:           |.:|.....|.|.:.:
Zfish   200 NVINPLLVTSTEDALMQARNSGVVGLRGQISPCSTHGSTGTLSSRDSLGGESTVSEPALSR---- 260

  Fly   165 YCAAHPKAIVILDKYKDELEKYMERQGAATPGLLVLTTGLSKP--------------FRRLDKYS 215
              :.....|..|.:...:.:|..:........||..:|.||.|              .|||.:.|
Zfish   261 --SLTDMGIGSLHREDSDSQKSPDSSSIRGHKLLSFSTDLSNPNMRKRHPRDSAAFESRRLFRKS 323

  Fly   216 AMLQELERHMESSHPDRGDTQRSVAVYKDIAATCSATRRQKELELQVLTGPVRGWQGQELSTLGD 280
            .| :.|:..|     :.|.:..|:......|:.      .||....|.....|||.|      ||
Zfish   324 YM-EALQNPM-----NLGSSSESILEEPSFASV------PKESMAPVRKICARGWLG------GD 370

  Fly   281 IIHMGSVAVGADHRDRYFVLFPQTLLFLSVSQRMSAFIYEGKLPLTGIIVNRLEDTDALKNA--- 342
            ....||:|..           |.....||.::|.|..:.........:..:|...:....|:
Zfish   371 ESRNGSLAGN-----------PSKNAELSPNERRSKSLERTNNKTLQVKGHRARSSSGGSNSGFP 424

  Fly   343 ---------------FEISSPLIDRIVA-----VC---QGPNEANKWVELLNANNPSL-PMGIKR 383
                           .|.:.|.::|..:     ||   .|...:|...|.::|..|:: |..:
Zfish   425 KKLINGYGFRFGKLELEAAFPGLERRSSKENSGVCSEWSGRRSSNSGDECVSAAPPAVSPSAL-- 487

  Fly   384 QLSNLSNSSLGHLNAAHLSQHLDSRGY---------------CT----RFSLCAYYSSPPCHVRP 429
            .:|.|....| :..|..|..:::..|.               ||    ...||.|.||.....|.
Zfish   488 MISQLDQELL-NSGALILPGNVERGGRAVVQVCCSKQVWSSDCTDQIISVLLCYYCSSLRKEKRE 551

  Fly   430 LRVT-LPPSNYPATAPYANLSA--HFARLVKGGGLRSAIVKMLLYPQARQSIDLKRIALRKRRCH 491
            |.:| |..:....|.|...:||  .|...|.     :|:..:|...:...::.::|.....   :
Zfish   552 LGLTVLVDARRQQTVPSTLISALSQFQTSVP-----NALYSVLFLVEKEAAVKVERDVTFP---Y 608

  Fly   492 KASAKLKDLNTNQDSGQSELERQDAIELPTDSESYDDDFEDDFLHSCDSDPFEYVQFYQN-KRND 555
            :....||.|..:.||.|          |.||   .|..|..|..|        |:.|.|. :...
Zfish   609 EVLTSLKALQKHIDSTQ----------LTTD---LDGTFPYDHSH--------YINFRQKIEPFA 652

  Fly   556 SMCNSTGTFVDHGTGARRHCSSI----NLIKLDSA-DTDEVLA--------------LNELKKES 601
            |.|::..:|:.         |||    |:..|::| :..||:.              ||.|:.|
Zfish   653 SSCSAAISFLQ---------SSIETLNNISNLETAQEVSEVIGQQKSLMKNILDDTELNRLRLE- 707

  Fly   602 LVIGSRALRALARKSTTRNSSVHTSTATLELGVGGSITNCVEEE----PILKVKPSFSLQQQSSD 662
               |...|..|.::....|   |.....::|  ..::.|.|:||    .||..|   |||
Zfish   708 ---GGTFLARLRKEEMCEN---HNYRDAVDL--VSALYNQVDEEVHKLVILSNK---SLQ----- 756

  Fly   663 ASSIFAARLGGAFTACENLASMPDDLSRESSIQEPPTPLPA---SPTERHSMP----TIFVGNRF 720
                             ||    :.|....:|||....:..   ..:|||..|    ::.:.:..
Zfish   757 -----------------NL----ETLLELRTIQEKQEEIKRWFYVESERHLAPLDSFSLSLSSIQ 800

  Fly   721 NHSKNTEVYVPTWRDRQEMQNQSVDAKQDEELHSSSIDLPAACLSAPDKL-----QAELLYN--- 777
            :.||:.|:::   ::.:|.||::....::.:..||.::|....:|...:.     |..:|:|
Zfish   801 DMSKSLELFM---QEAREQQNKASVLLRESDSSSSLLELNQQIISVLQRCETRRSQLHVLHNLYE 862

  Fly   778 -YDEILEKPLQ----------------------LHRELTPFPGHNLNSDKRVSHKSDSPSTGNAK 819
             |....|..||                      .|.|...|...|.:|.::...:.:||  |..:
Zfish   863 FYQSANEWMLQCQEYLCRLRVDGDAAALQMLQDFHSEAQQFSPQNFSSFQQTLQQLESP--GEEQ 925

  Fly   820 TDPNLATRSSSTT----ELCIDTTSKKRTTPSERSRDSIRRCISYQFLQMSNRPPPPPPPRRDPD 880
            ...|: .||...|    |..:::::.:.||                  |:.:.||........||
Zfish   926 RWRNI-WRSCKHTQQQLEELMESSTPQHTT------------------QVFSSPPESLNGGVSPD 971

  Fly   881 LHLDTKCRCCENSQCPS-PRS------SDSGMAGSCTI-TSPDPPN------------PESYFPM 925
              |.....|.|....|. |||      :||    .||: :||..|:            ..||.|.
Zfish   972 --LPPLSSCVEYDDAPQFPRSLSTAEDTDS----DCTVDSSPSEPHRLRKQPLKKIMKTLSYEPS 1030

  Fly   926 EAAGH--------DMLDNVEPE----RFDVCG--MFREKFL-TPEATQDVVDLSEEQPQLSDEPT 975
            ...|:        ::.:||..|    |.||..  :.|.:.| ||........:|.:...:.||..
Zfish  1031 AHHGYTGVYIRGLEVSNNVSAEKKLQRPDVKSPVLTRSRSLSTPCRADHCHSVSSKVQHIMDEMI 1095

  Fly   976 TPTNRKEEPTCITSAQVQVNTRSIFLPSSSSMDETNRNVPANNILFSSSSADQLEPQATFRS--- 1037
            : |.|:...:.|...|.       :.|....:|.........:|:|.:     ||..:.|.|
Zfish  1096 S-TEREYVRSLIYVVQQ-------YFPEMERLDLPQDLRGKRSIIFGN-----LEKLSDFHSLYF 1147

  Fly  1038 -------------------------GMYAHWWKKERLPPEVVRGIAHAY--NKSLPSKDSKDSGS 1075
                                     ||||.:.|.:.....::....:|:  ||.:...|..|..|
Zfish  1148 LKELERCSHSPLTVSRCFLMHEEQFGMYALYSKNKPRSDALLTSHGNAFFRNKQVELGDKMDLAS 1212

  Fly  1076  1075
            Zfish  1213  1212

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
RtGEF	NP_001097184.2	SH3_PIX	6..58	CDD:212810
		RhoGEF	79..249	CDD:238091	41/210 (20%)
		PH_Cool_Pix	281..376	CDD:269932	19/120 (16%)
plekhg4b	XP_073780503.1	None