Protein Alignment neb and KIF4A

DIOPT Version :10

• Sequence 1: NP_476817.1
• Gene: neb / 35293
• FlyBaseID: FBgn0004374
• Length: 1121
• Species: Drosophila melanogaster
• Sequence 2: NP_036442.3
• Gene: KIF4A / 24137
• HGNCID: 13339
• Length: 1232
• Species: Homo sapiens
• Alignment Length: 1072
• Identity: 285/1072 - (26%)
• Similarity: 442/1072 - (41%)
• Gaps: 266/1072 - (24%)

  Fly   123 VAVRVRPLNALECTRGQVTNVVQVHGNSNELTVQAGSSADASAGVTHFFSYDQVYYSCDPERKNF 187
            ||:|.|||...|.:.|....:..|.|....:           .|....|:||.|:   ||..:
Human    12 VALRCRPLVPKEISEGCQMCLSFVPGEPQVV-----------VGTDKSFTYDFVF---DPSTE-- 60

  Fly   188 ACQAKVFEGTARPLIDTAFEGYNACLFAYGQTGSGKSYSMMGIEALDDAALDGGPPHDEAGIIPR 252
              |.:||.....|||...|:||||.:.|||||||||:|||.|...   |..:..|   ..|:|||
Human    61 --QEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYT---AEQENEP---TVGVIPR 117

  Fly   253 FCHELFRRIEAVKSQQQLQVEVEVSYFEIYNEKIHDLLSVQHAAAATGESTPIQQQQQQQRPALK 317
            ....||:.|:   .:...:..::|||.|||||:|.|||            .|.:::.|     :.
Human   118 VIQLLFKEID---KKSDFEFTLKVSYLEIYNEEILDLL------------CPSREKAQ-----IN 162

  Fly   318 VREHPIFGPYVVDLSAHSV----DSYSALRNWLAVGNSQRATASTAMNDKSSRSHSIFNIVLNLT 378
            :||.|..|..:|.|:..:|    |:.|.|..    ||:.|..||||||.:|||||:||.|
Human   163 IREDPKEGIKIVGLTEKTVLVALDTVSCLEQ----GNNSRTVASTAMNSQSSRSHAIFTI----- 218

  Fly   379 DLSSDDGLSSDTDSSTASSLRQTRRSKISLVDLAGSERISVSGSNGERIREGVSINKSLLTLGKV 443
              |.:....||.:||.        |||:.|||||||||...:.:.|:|::||::||:.||.||.|
Human   219 --SLEQRKKSDKNSSF--------RSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNV 273

  Fly   444 IAALADSRKAIANGPLGSGTPSTFVPYRESVLTWLLRENLGGNSKTVMLATISPASIHADETLAT 508
            |:||.|.:|            ..|||||:|.||.||:::|||||.|:|:|.:|||..:.:|||.|
Human   274 ISALGDDKK------------GGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNT 326

  Fly   509 LRYACKARSIVNRVKVNESPHDKIIRDLRAEVDRLKSLRNEYERQRRLSGNSNNPVPRKIIIETS 573
            ||||.:||.|.|:..||..|....:..|:.:|.:|:.|         |.......:|..|.:|.|
Human   327 LRYADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQVL---------LLQAHGGTLPGSITVEPS 382

  Fly   574 VDETEVEALRQQLAERERELSRAQKSWMEKLKEAEDQRKSEL-RVLKRRGLALELTAE-----QK 632
            .:...:....|.|.|...:|||.       |.||..|....| |::.......::.|:     |.
Human   383 ENLQSLMEKNQSLVEENEKLSRG-------LSEAAGQTAQMLERIILTEQANEKMNAKLEELRQH 440

  Fly   633 QACLVNLTADPILSGTLFYLLPQGLVRIGRGRLPGGSSSSQPDIV---------LDGPLVALQHC 688
            .||.::|               |.||.    .|.........:|:         |....||....
Human   441 AACKLDL---------------QKLVE----TLEDQELKENVEIICNLQQLITQLSDETVACMAA 486

  Fly   689 SIEH--ERGGKLYVIP----GSEDFET-------YVNGELLKDRRQLFHGDRLVIGGSHYFRISN 740
            :|:.  |:..::...|    .|:.|.|       .::.||::..:.|...:.|.
Human   487 AIDTAVEQEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKALALKEALA----------- 540

  Fly   741 PFCSQRGKADHPVDFQ----LAHQEI----LQKQEQQLRSELE-AEKRAALTKIEQERAQHARDF 796
            ...:|......|:.:|    :...|:    |||::::|..||: |:|.|...|:.:.|.:..::.
Human   541 RKMTQNDSQLQPIQYQYQDNIKELELEVINLQKEKEELVLELQTAKKDANQAKLSERRRKRLQEL 605

  Fly   797 E-----------ERLQCLEL----EQFKYKCNSEMLETERQALALAQQQEHTPLRHEDAVSTPAQ 846
            |           |:.:.|:|    |:...|.|.|:...:.|.:.|.:|.:
Human   606 EGQIADLKKKLNEQSKLLKLKESTERTVSKLNQEIRMMKNQRVQLMRQMK--------------- 655

  Fly   847 KSTILEDIQRIMLNPSEESLHKTQLMVKEATQRCRQLDLPLEFR------QTQTPDEFGLLRTVI 905
                 ||.::......::.....||..::..::...|.|...|:      :.:|.:.....:.:.
Human   656 -----EDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLK 715

  Fly   906 LILDKQRGLK----------AEWPTARLGVWLDLVRDNAEQQEKLNATTIFQSVEVDWEPLDADL 960
            ..|.|||.:.          .|...||:..||      ..:.|          |.|..|.....|
Human   716 DALQKQREVADKRKETQSRGMEGTAARVKNWL------GNEIE----------VMVSTEEAKRHL 764

  Fly   961 NETLSDTHNSSRIALNLSAMKDVLLNKP--LKRLL--------NASSSKSNTPRQ-----TSTPS 1010
            |:.|.|....::....|...|:...|.|  |:|..        ..|.|:.:..:|     |....
Human   765 NDLLEDRKILAQDVAQLKEKKESGENPPPKLRRRTFSLTEVRGQVSESEDSITKQIESLETEMEF 829

  Fly  1011 PGSQLVHFTKRLLTYDPEEIPESQ-SSFTT--------------LVQQELQI--MQRSAQRLRRH 1058
            ..:|:....::||..:.|:.|:.: .:..|              ||..::|:  ::.|.::.:..
Human   830 RSAQIADLQQKLLDAESEDRPKQRWENIATILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTS 894

  Fly  1059 C---EAALLERENHQDNGVLAVSLQEALARMD 1087
            |   :..|.|..||  ...:...||..|.||:
Human   895 CADMQKMLFEERNH--FAEIETELQAELVRME 924

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
neb	NP_476817.1	KISc_KIF1A_KIF1B	119..525	CDD:276816	157/405 (39%)
		SMC_N	514..>615	CDD:481474	26/100 (26%)
		FHA_KIF14	627..741	CDD:438759	23/140 (16%)
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	155/399 (39%)
		PRK11281	383..>646	CDD:481316	58/299 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..515		4/18 (22%)
		Smc	<517..>1033	CDD:440809	85/457 (19%)
		Required for the interaction with PRC1. /evidence=ECO:0000269|PubMed:15297875	663..1232		53/280 (19%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:11736643	793..798		1/4 (25%)
		Globular	1000..1232
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269|PubMed:29848660	1086..1144
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1122..1142