Protein Alignment neb and KIF21B

DIOPT Version :10

• Sequence 1: NP_476817.1
• Gene: neb / 35293
• FlyBaseID: FBgn0004374
• Length: 1121
• Species: Drosophila melanogaster
• Sequence 2: NP_001239029.1
• Gene: KIF21B / 23046
• HGNCID: 29442
• Length: 1637
• Species: Homo sapiens
• Alignment Length: 1193
• Identity: 290/1193 - (24%)
• Similarity: 458/1193 - (38%)
• Gaps: 362/1193 - (30%)

  Fly   123 VAVRVRPLNALECTRGQVTNVVQVHGNSNELTVQAGSSADASAGVTHFFSYDQVY---------Y 178
            ||||:||..:.|          ::.|.....:|..| ......|....|:||.|:         |
Human    11 VAVRIRPQLSKE----------KIEGCHICTSVTPG-EPQVLLGKDKAFTYDFVFDLDTWQEQIY 64

  Fly   179 SCDPERKNFACQAKVFEGTARPLIDTAFEGYNACLFAYGQTGSGKSYSMMGIEALDDAALDGGPP 243
            |        .|.:|:.||        .||||||.:.||||||:||:|:|       ....|....
Human    65 S--------TCVSKLIEG--------CFEGYNATVLAYGQTGAGKTYTM-------GTGFDMATS 106

  Fly   244 HDEAGIIPRFCHELFRRIEAVKSQQQLQ------VEVEVSYFEIYNEKIHDLLSVQHAAAATGES 302
            .:|.|||||....||..|...|.:.|.|      .:|...:.|:|||:|.||.          :|
Human   107 EEEQGIIPRAIAHLFGGIAERKRRAQEQGVAGPEFKVSAQFLELYNEEILDLF----------DS 161

  Fly   303 TPIQQQQQQQRPALKVREHPIFGPYVVDLSAHSVDSYSALRNWLAVGNSQRATASTAMNDKSSRS 367
            |. ....:.:|..:|:.|....|.|...:::..:.|...|...|..|...|.||||.||.:||||
Human   162 TR-DPDTRHRRSNIKIHEDANGGIYTTGVTSRLIHSQEELIQCLKQGALSRTTASTQMNVQSSRS 225

  Fly   368 HSIFNIVLNLTDLSSDDGLSSDT-----DSSTASSLRQTRRSKISLVDLAGSERISVSGSNGERI 427
            |:||.|.|....:.:...|.::.     |.:..||..:|..:|...||||||||:..:|:.|||.
Human   226 HAIFTIHLCQMRMCTQPDLVNEAVTGLPDGTPPSSEYETLTAKFHFVDLAGSERLKRTGATGERA 290

  Fly   428 REGVSINKSLLTLGKVIAALADSRKAIANGPLGSGTPSTFVPYRESVLTWLLRENLGGNSKTVML 492
            :||:|||..||.||.||:||.|..|.:.:           ||||:|.||.||:::|||||:|:|:
Human   291 KEGISINCGLLALGNVISALGDQSKKVVH-----------VPYRDSKLTRLLQDSLGGNSQTIMI 344

  Fly   493 ATISPASIHADETLATLRYACKARSIVNRVKVNESPHDKIIRDLRAEVDRLKSLRNEYERQRRLS 557
            |.:||:.....|||.||:||.:||:|.|:|.||:....:.|..||||:.||:....||:..:|:.
Human   345 ACVSPSDRDFMETLNTLKYANRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVI 409

  Fly   558 GNS------------------------------------NNPVPRKIIIETSV--------DET- 577
            |..                                    ||.|.:.:..|.::        :|.
Human   410 GEDGAEGYSDLFRENAMLQKENGALRLRVKAMQEAIDAINNRVTQLMSQEANLLLAKAGDGNEAI 474

  Fly   578 ---------EVEALRQQLAERE-------RELSRAQ----------------------------- 597
                     |:|.||.:|.|.|       |.||||.
Human   475 GALIQNYIREIEELRTKLLESEAMNESLRRSLSRASARSPYSLGASPAAPAFGGSPASSMEDASE 539

  Fly   598 -----KSWMEKL--KEAEDQRKS-ELRVLKRRGLALELTAEQ--------------KQAC----- 635
                 |..:|:|  ||...:||| |....|:|....:..:|:              :..|
Human   540 VIRRAKQDLERLKKKEVRQRRKSPEKEAFKKRAKLQQENSEETDENEAEEEEEERDESGCEEEEG 604

  Fly   636 -------------LVNLTADPILSGTLFYLLPQGLVRIGRGRLPGGSSSSQPDIVLDGPLVALQH 687
                         ||:..:||                            .:.::.....|..|.
Human   605 REDEDEDSGSEESLVDSDSDP----------------------------EEKEVNFQADLADLT- 640

  Fly   688 CSIEHERGGKLYVIPGSEDFETYVNGELLKDRRQL-----FHGDRLVIGGSHYFRISNPFCSQRG 747
            |.||.::  ||.             .||...:|:|     .:.::|::       :.|..
Human   641 CEIEIKQ--KLI-------------DELENSQRRLQTLKHQYEEKLIL-------LQNKI----- 678

  Fly   748 KADHPVDFQLAHQEILQK--------QEQQLRSELEAEKRAA-----LTKIEQERAQHARDFEER 799
                 .|.||....:||.        :|:..:.:.:.|||..     |.|::..:.:|||
Human   679 -----RDTQLERDRVLQNLSTMECYTEEKANKIKADYEKRLREMNRDLQKLQAAQKEHAR----- 733

  Fly   800 LQCLELEQFKY-----KCNSEMLETERQALALAQQQEHTPLRHEDAVSTPAQKSTILEDIQRIML 859
               |...|.:|     |..:|:.|.::..:||.:|     :|.|.      |:..::|..:...:
Human   734 ---LLKNQSRYERELKKLQAEVAEMKKAKVALMKQ-----MREEQ------QRRRLVETKRNREI 784

  Fly   860 NPSEESLHKTQLMVKEATQRCRQLDLPLEFRQTQTPDEFGLLRTVILILDK----QRGLKAEWPT 920
            ...::...:.:..::....:.||.::.|. |:||   |...||.:...:.:    :.|||.  |.
Human   785 AQLKKEQRRQEFQIRALESQKRQQEMVLR-RKTQ---EVSALRRLAKPMSERVAGRAGLKP--PM 843

  Fly   921 ARLGVWLDLVRDNAEQQEKLNATTIFQSVEVDWEPLDADLNETLSD----THNSSRIALNLSAMK 981
            ...|..:.....::|.:....:.:   |:...|   :..:|..|.|    |.|.:|.|......|
Human   844 LDSGAEVSASTTSSEAESGARSVS---SIVRQW---NRKINHFLGDHPAPTVNGTRPARKKFQKK 902

  Fly   982 DVLLNKPLKRLLNASSSKSNTPRQTSTPSPGSQLVHFTKRLLTYDPEEIPESQSSFTTLV--QQE 1044
                        .||.|.|...| ....|...:::....:.:|     |...::....|:  ::|
Human   903 ------------GASQSFSKAAR-LKWQSLERRIIDIVMQRMT-----IVNLEADMERLIKKREE 949

  Fly  1045 LQIMQRSAQRLRRHCEAALLERENHQDNGVLAVSLQEALARMDTVLNGM---HTSLARAEATACV 1106
            |.::|.:.:|.|...:|...|.|.....  ||..::...|.:|.:.:|:   ..::.:.|.|...
Human   950 LFLLQEALRRKRERLQAESPEEEKGLQE--LAEEIEVLAANIDYINDGITDCQATIVQLEETKEE 1012

  Fly  1107 TSPTKTQKAV--------RFLID 1121
            ...|.|...:        |.|:|
Human  1013 LDSTDTSVVISSCSLAEARLLLD 1035

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
neb	NP_476817.1	KISc_KIF1A_KIF1B	119..525	CDD:276816	149/421 (35%)
		SMC_N	514..>615	CDD:481474	43/198 (22%)
		FHA_KIF14	627..741	CDD:438759	18/150 (12%)
KIF21B	NP_001239029.1	KISc_KIF4	7..371	CDD:276823	146/415 (35%)
		CwlO1	379..>539	CDD:443091	28/159 (18%)
		Interaction with TRIM3. /evidence=ECO:0000250|UniProtKB:Q9QXL1	400..1099		132/748 (18%)
		Smc	<472..>1064	CDD:440809	124/676 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	509..538		1/28 (4%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	552..628		14/103 (14%)
		EnvC	633..>842	CDD:443969	52/266 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	830..865		6/36 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	880..906		8/37 (22%)
		Rcc_KIF21B	929..1010	CDD:410203	17/87 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1194..1251
		WD40	1305..1613	CDD:238121
		WD 1	1306..1343
		WD40 repeat	1312..1346	CDD:293791
		WD 2	1346..1384
		WD40 repeat	1351..1387	CDD:293791
		WD40 repeat	1407..1450	CDD:293791
		WD 3	1410..1448
		WD 4	1451..1493
		WD40 repeat	1456..1500	CDD:293791
		WD 5	1502..1539
		WD40 repeat	1509..1541	CDD:293791
		WD 6	1543..1582
		WD40 repeat	1548..1572	CDD:293791
		WD 7	1585..1622