Protein Alignment neb and kif27

DIOPT Version :10

• Sequence 1: NP_476817.1
• Gene: neb / 35293
• FlyBaseID: FBgn0004374
• Length: 1121
• Species: Drosophila melanogaster
• Sequence 2: XP_031750817.1
• Gene: kif27 / 100497455
• XenbaseID: XB-GENE-6039606
• Length: 1393
• Species: Xenopus tropicalis
• Alignment Length: 1059
• Identity: 258/1059 - (24%)
• Similarity: 439/1059 - (41%)
• Gaps: 277/1059 - (26%)

  Fly   117 EESNMIVAVRVRPLNALE-------CTRGQVTNVVQVHGNSNELTVQAGSSADASAGVTHFFSYD 174
            ||:.:.||:|||||.:.|       |.| .|.|..||                 ..|....|::|
 Frog    14 EETPVKVALRVRPLLSREILHNHQVCVR-LVPNTQQV-----------------IVGKDRVFTFD 60

  Fly   175 QVYYSCDPERKNFACQAKVFEGTARPLIDTAFEGYNACLFAYGQTGSGKSYSMMG--IEALDDAA 237
            .|:       ...:.|..|:..:.:||:.:..|||||.:||||||||||:|::.|  :.::.|
 Frog    61 FVF-------GKSSSQDDVYRSSIKPLLGSLMEGYNATVFAYGQTGSGKTYTIGGGNVASVAD-- 116

  Fly   238 LDGGPPHDEAGIIPRFCHELFRRIEAVKSQQQLQVEVEVSYFEIYNEKIHDLLSVQHAAAATGES 302
                   :|.|||||...|||   :.:..:..::..|.|||.|:|.|::.|||.::
 Frog   117 -------EEKGIIPRAIQELF---QCIAERHNVEFSVRVSYIEVYKEELLDLLELE--------- 162

  Fly   303 TPIQQQQQQQRPALKVREHPIFGPYVVDLSAHSVDSYSALRNWLAVGNSQRATASTAMNDKSSRS 367
            |.::.        |.:||.......:|......|::...:.:.||.|::.|.|.||.||::||||
 Frog   163 TNMKD--------LHIREDEKGNTVIVGAKECQVENADEVMSLLAAGSAARHTGSTQMNERSSRS 219

  Fly   368 HSIFNIVLNLTDLSSDDGLSSDTDSSTASSLRQTRRSKISLVDLAGSERISVSGSNGERIREGVS 432
            |:||.|  .:...||..|..:.|:|.      ||..||...||||||||::.:|:.|||.:|.:.
 Frog   220 HAIFTI--TICQQSSQHGDDAGTNSI------QTITSKFHFVDLAGSERVTKTGNTGERFKESIQ 276

  Fly   433 INKSLLTLGKVIAALADSRKAIANGPLGSGTPSTFVPYRESVLTWLLRENLGGNSKTVMLATISP 497
            ||..||.||.||:||.|.::           .|..:|||::.:|.:|:::||||:||||:..|||
 Frog   277 INSGLLALGNVISALGDPKR-----------KSAHIPYRDAKITRILKDSLGGNAKTVMITCISP 330

  Fly   498 ASIHADETLATLRYACKARSIVNRVKVNESPHDKIIRDLRAEVDRLKSLRNEYERQRRLSGNSNN 562
            :|...||:|.:|:||.:||:|.|:..||.:|          :.||:..:..|.:..|
 Frog   331 SSSDLDESLNSLKYANRARNIKNKPVVNYNP----------DWDRMDEMELEIKVLR-------- 377

  Fly   563 PVPRKIIIETSVDETEVEALRQQLAERERELSRAQKSWMEKLKEAEDQRKSELRVLKRRGLALEL 627
                             |||:.|.:.|....|:..:.|        .|.|.::|.|:.:...|:|
 Frog   378 -----------------EALQNQQSSRVSRTSQVSQDW--------SQGKDKIRSLEEKVAQLQL 417

  Fly   628 TAEQKQAC---LVNLTAD-PILSGTLFYLLPQG-------LVRIGRG-RLP-----GGSSSSQPD 675
            ....::.|   ..:|..| ..:||     |||.       .:.:..| |.|     .|||..:|.
 Frog   418 ECFNQRHCTEEAFSLFLDLKDISG-----LPQSEKNRVQDWLHLAEGLRSPVAADAEGSSREEPH 477

  Fly   676 IVLDGPLV-ALQHCSIEHERGGKLYVIPGSEDFETYVNGELLKDRRQLFHGDRLVIGGSHYFRIS 739
            .|....|. .|:.|.                        :.|....::|...:|.:.|
 Frog   478 HVTILQLKRELKKCQ------------------------DALATDEEVFARKKLELKG------- 511

  Fly   740 NPFCSQRGKADHPVDFQLAHQEILQKQEQQLRSELEAEKRAALTKIEQERA-QHARDFEERLQCL 803
                                   ||:|.|.|:.|.| |.:..|.:.|..:. |..:..|::|...
 Frog   512 -----------------------LQEQIQSLQQETE-EYQKCLQEAEYTKTLQTEKLVEQQLLID 552

  Fly   804 ELEQFK---YKCNSEMLETERQALALAQQQEHT--PLRHEDAVSTPAQKSTILEDIQRIMLNPSE 863
            ||:..|   |:..|:..|.|.:......|:.||  |..|...:|:.:.::....:.:::..:|..
 Frog   553 ELKSKKPEIYRQFSQENEDEEELPREISQRPHTVPPSTHTQKLSSSSLQAAGQAEPRKVRTSPPS 617

  Fly   864 ESLH--------KTQLMVKEATQRCRQLDLPLEFRQTQTPDEFGLLRTVILILDKQ--------R 912
            .||.        ::|::::...::...|......:..:.|||          .:|:        |
 Frog   618 YSLERMVAGFRTRSQMLLELIEEQDEVLHEKFSDQSEEEPDE----------PEKENRKKSPFRR 672

  Fly   913 GLKAEWPTARLGVWLDLVRDNAEQQEKLNAT------TIFQSVEVDWEPLDADLNETLSDTHNSS 971
            .:...|...:.....:.:..|::::...|:.      ::.:|..::.|.|... :..|:|.....
 Frog   673 SINRTWTRRQGSAGAETLNSNSDRENDANSNACPALESLRESQVLNVEKLKTS-DVRLADAKQKM 736

  Fly   972 R-IALNLSAMKDVLLNKPLKRLLNASSSKSNTPRQTSTPSPGSQLVHFTKRLLTYDPEEIPESQS 1035
            | :.:|:. ||:.|    :|.|:...:...:..||.|.     ::.|..           .|::.
 Frog   737 RELTINIK-MKEEL----IKELIKTGNDAQSVNRQYSL-----KIAHLE-----------GEAEQ 780

  Fly  1036 SFTTLVQQELQIMQRSAQRLRRHCEAALLERENHQDNGVLAVSLQEALARMDTVLNGMHTSLARA 1100
            :.:.|.:.:.|:.:...:.||.......|:|:..:       .::||..:|..|      ...:.
 Frog   781 AKSELSEAQRQLQELENKELRDVAHKTKLQRDFRK-------KMEEAKLKMQAV------QTKQQ 832

  Fly  1101 EATACVTSPTKTQKAVRFL 1119
            :.....:...:.:|.||.|
 Frog   833 DTRKLASLSVRNEKRVREL 851

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
neb	NP_476817.1	KISc_KIF1A_KIF1B	119..525	CDD:276816	143/414 (35%)
		SMC_N	514..>615	CDD:481474	20/100 (20%)
		FHA_KIF14	627..741	CDD:438759	24/131 (18%)
kif27	XP_031750817.1	KISc_KIF4	16..352	CDD:276823	141/408 (35%)
		SMC_prok_B	<363..>586	CDD:274008	62/315 (20%)
		SMC_prok_B	<481..900	CDD:274008	77/471 (16%)
		Smc	<707..>1237	CDD:440809	31/180 (17%)