Protein Alignment sick and unc-53

DIOPT Version :9

• Sequence 1: NP_001260606.1
• Gene: sick / 35277
• FlyBaseID: FBgn0263873
• Length: 2707
• Species: Drosophila melanogaster
• Sequence 2: NP_001022170.1
• Gene: unc-53 / 174693
• WormBaseID: WBGene00006788
• Length: 1654
• Species: Caenorhabditis elegans
• Alignment Length: 2253
• Identity: 494/2253 - (21%)
• Similarity: 774/2253 - (34%)
• Gaps: 777/2253 - (34%)

  Fly   536 IYTDWANYYLERAKSKRKVTDLSADCRDGLLLAEVIEAVTSFK--VPDLVKKPKNQQQMFDNVNS 598
            |||||||.:|.:....:.:.|:|.|.||..|::::|..:....  .|...|:........|.:.:
 Worm    11 IYTDWANRHLSKGSLSKSIRDISNDFRDYRLVSQLINVIVPINEFSPAFTKRLAKITSNLDGLET 75

  Fly   599 CLHVLRSQSVGGLE--NITTNDICAGRLKAVLALFFALSRFKQQAKQTKSIGVGCGGGVGGSSST 661
            ||..|::.   ||:  .:|..||.:|.|.|||.|.|.||.:||:.:|.|.          .....
 Worm    76 CLDYLKNL---GLDCSKLTKTDIDSGNLGAVLQLLFLLSTYKQKLRQLKK----------DQKKL 127

  Fly   662 LTGSGSVLGIGIGGLRTP-------GSSLNQDKNQQEQQQQQQQQQTPQQLAQSLENGNEMVNRQ 719
            .....|::...:..|.:|       .|:.|.:.|  ..|....:.||||.....:::....:.
 Worm   128 EQLPTSIMPPAVSKLPSPRVATSATASATNPNSN--FPQMSTSRLQTPQSRISKIDSSKIGIK-- 188

  Fly   720 IAPAYAKVNGGTAIPLPATVMVQRRCPPDKVRPLPPTPNHTPSIPGLGKSGSDFNTSRPNSPPTS 784
                            |.|..::           ||:.:.|        |.::.|:.||:|..:.
 Worm   189 ----------------PKTSGLK-----------PPSSSTT--------SSNNTNSFRPSSRSSG 218

  Fly   785 NHTIQSLKSGNNNSLRPPSIKSGIPS----------PSSPQTAPQKHSMLDKLKLFNKEKQQNAV 839
            |:.:.|..|.:..||...|..|.|.:          ||.|||...:.:...|:. .:|.....||
 Worm   219 NNNVGSTISTSAKSLESSSTYSSISNLNRPTSQLQKPSRPQTQLVRVATTTKIG-SSKLAAPKAV 282

  Fly   840 NAASVASKTQIQSKRTSSSSGFSSARSERSDSSLSLNDGHGSQLK-----PPSISVSSQKPQPKT 899
            :...:||...|.:|:...:||                .|.|..||     ..:.|.||..|||..
 Worm   283 STPKLASVKTIGAKQEPDNSG----------------GGGGGMLKLKLFSSKNPSSSSNSPQPTR 331

  Fly   900 KQSKL--------LAAQQKK------EQANKATKLDK-------KEKSPARSLNKEESGNESRSS 943
            |.:.:        :||..|.      .:...||.:.|       ..|:.|..:::::|...|:||
 Worm   332 KAAAVPQQQTLSKIAAPVKSGLKPPTSKLGSATSMSKLCTPKVSYRKTDAPIISQQDSKRCSKSS 396

  Fly   944 --TMGRTGKSSLVRAVGGVEKNTPKTSSKS----SLHSKSDSKSSLKAPQLLQSPSSGGLP---- 998
              ..|..|.:|           |..|||.:    |:||.| ||||....   :||||..|.
 Worm   397 EEESGYAGFNS-----------TSPTSSSTEGSLSMHSTS-SKSSTSDE---KSPSSDDLTLNAS 446

  Fly   999 ------KPIAAIKGTSKLPSLGGGAGHLPAAESQQNQQLLKRETSDI----SSNISQPPPAEPPI 1053
                  :||||                .|.:.:..|:.:.::.|..:    |:....||||.||.
 Worm   447 IVTAIRQPIAA----------------TPVSPNIINKPVEEKPTLAVKGVKSTAKKDPPPAVPPR 495

  Fly  1054 STHAHIHQNQTPPPPYYANSQPT----SHISSH------GFLSEPSTPQHSSGIYGSSR----LP 1104
            .|                  |||    |.|.:|      ..:||...|:....:...:.    ||
 Worm   496 DT------------------QPTIGVVSPIMAHKKLTNDPVISEKPEPEKLQSMSIDTTDVPPLP 542

  Fly  1105 PPKSALSAPRKLEYNAGPHILSSPTHH--QRQGLPRPLVNSAPNTPTASPNKFHTIPSKIVGTIY 1167
            |.||.:  |.|:     ..|...||:.  .:||                  |. |.|.|..|...
 Worm   543 PLKSVV--PLKM-----TSIRQPPTYDVLLKQG------------------KI-TSPVKSFGYEQ 581

  Fly  1168 ESKEEQLLPAPPPASGGSSILPMRPLLRGYNSHVTLPTRGARGGHHPHQSYLDFCESDIGQGYCS 1232
            .|..|..:.|...|                  .||.||:  ..|:|..:..:...::....||.|
 Worm   582 SSASEDSIVAHASA------------------QVTPPTK--TSGNHSLERRMGKNKTSESSGYTS 626

  Fly  1233 D-GDALRVGSSPGGSRFHDI----DNGYLSEGSSGLNGPSSSAGGISPGKHFLSMMRARTQLPTT 1292
            | |.|:..........:.|:    .|||    .......||.:.|||
 Worm   627 DAGVAMCAKMREKLKEYDDMTRRAQNGY----PDNFEDSSSLSSGIS------------------ 669

  Fly  1293 IEERIRNSRGSLDSIGT-AANGSSAASQASSSGGSTTTHAQNNNNNNNNNSGGGAKMDGSPHHRP 1356
                   ....||.|.| ..:|...|:.||
 Worm   670 -------DNNELDDISTDDLSGVDMATVAS----------------------------------- 692

  Fly  1357 GSRNGRDNWSKMPEPLNGQKVEKSDKSSPSRRSMGGGGSGSSSKQGSPSSSSRTKGVPPSFGYVK 1421
                                 :.||.|...|.               |:|||....||      .
 Worm   693 ---------------------KHSDYSHFVRH---------------PTSSSSKPRVP------S 715

  Fly  1422 RANGSI--ASTAEQQNIAMMMAAGGAGANGLPCGRTAHVSAVPRTASGRKVAGGTQTLPNDMNKL 1484
            |::.|:  .|.|||:|:..:::                     :..:.::.|..|.|.
 Worm   716 RSSTSVDSRSRAEQENVYKLLS---------------------QCRTSQRGAAATSTF------- 752

  Fly  1485 PPNTQHRSFSLTGPTATQLSQSIRERLATGSHSLPKPGSDLHVFQHRISNRGGTRHDGSLSDTQT 1549
                                         |.|||..||...:.....:|....|....|.:..:.
 Worm   753 -----------------------------GQHSLRSPGYSSYSPHLSVSADKDTMSMHSQTSRRP 788

  Fly  1550 YAEVKPEYSSYAMWLKHSNTAGSRLSDGESVEQLQIGSPALTRHGHKMIHNRSGGPGQMAGQMSG 1614
            .:: ||.||...    ||......|.:..|.|             |:|....|  |.::...||.
 Worm   789 SSQ-KPSYSGQF----HSLDRKCHLQEFTSTE-------------HRMAALLS--PRRVPNSMSK 833

  Fly  1615 NESPYVQSPRMNRSNSIRSTKSEKMYPSMMSRAGEVEIEPYYCLPVGTNGVLTAQMAAAMAAQSQ 1679
            .:|   .:..:|.|...||.   .:..|:..|.......|      ..:.::||..:|...:.|
 Worm   834 YDS---SAAALNASGMSRSM---ILLESLSPRPPRRHQSP------ADSCIITASPSAPRRSHS- 885

  Fly  1680 AAQGNPGVGVNVGGVAWSQPTSPTPLTRGPFNTAAGASVLSPTH-----GTTSAAGLVGPGGGAG 1739
                               |..||  .|.|.:.|:     ||.|     |:.||....|...|..
 Worm   886 -------------------PRGPT--ARIPLSLAS-----SPVHVNNNWGSYSARSRGGSSTGIY 924

  Fly  1740 GGAMVGHRLTYPKKNDEVH------GSAASLLSGGSSLYGNAEERQAHEIRRLKRELQDARDQVL 1798
            |.....|||:..|  ...|      ||..||.|  ::.||:..|:..|.||.:.|:|:..::.|.
 Worm   925 GETFQLHRLSDEK--SPAHSAKSEMGSQLSLAS--TTAYGSLNEKYEHAIRDMARDLECYKNTVD 985

  Fly  1799 SLSSQLSTNAHVVTAFEQSLSNMTNRLHQLTATAERKDGELTDMRQTIELLRKQSIQAGLTTAHM 1863
            ||:.:......:...|||.|..:|..:.:    :..|..|....||.|..||..|......:||
 Worm   986 SLTKKQENYGALFDLFEQKLRKLTQHIDR----SNLKPEEAIRFRQDIAHLRDISNHLASNSAH- 1045

  Fly  1864 QSMGVQTQGQGQVQGQALGQALGQPGSDQKPPNSGSQRAINANNGSMPLGMQRQHSTDSMCSLNS 1928
                                                     ||.|:..|  .||.|.:|:.|..|
 Worm  1046 -----------------------------------------ANEGAGEL--LRQPSLESVASHRS 1067

  Fly  1929 ISSGCSAAQDKNK------ANKKKGWLRSSFTKAFSRNAKISKTSRHVGHHHHHNHSQQELASGK 1987
            ..|..|.:..:.|      ...||.|:|||.:|...:..|              |:.:..:
 Worm  1068 SMSSSSKSSKQEKISLSSFGKNKKSWIRSSLSKFTKKKNK--------------NYDEAHM---- 1114

  Fly  1988 LPLHNGHGEPLGLASLATQPAPPLPNSKQSSPAKTVTLIDNAKPIDAIDQEDQHVVEDLKKQLRE 2052
                                 |.:..|:.:        :||   ||.|         :||::|:|
 Worm  1115 ---------------------PSISGSQGT--------LDN---IDVI---------ELKQELKE 1138

  Fly  2053 KDLVLTDIRLEALSSASQLESLKEMMNKMRAEMMSLKHNNERLQKLVTTRSLAGSEASLGQAISP 2117
            :|..|.::||:.|..|.:::.|:|.:||::.|...||...::|.....||  |.|.||
 Worm  1139 RDSALYEVRLDNLDRAREVDVLRETVNKLKTENKQLKKEVDKLTNGPATR--ASSRAS------- 1194

  Fly  2118 NGSVAGSSEVSRRYSLADSNSRPPMELPARLSEELELEEDCLPPAPAPEQPPPPAPNGVSVAPLS 2182
                                      :|....:|...:..|...:.:.........|.:.|
 Worm  1195 --------------------------IPVIYDDEHVYDAACSSTSASQSSKRSSGCNSIKV---- 1229

  Fly  2183 PSTHVDLTPPPPALEAAPMASPVHMASATEELADVCDGKKIAIACYLGQPEAFAKYCEELQELDG 2247
             :.:||:                     ..|::.:.:..|..|..||..|
 Worm  1230 -TVNVDI---------------------AGEISSIVNPDKEIIVGYLAMP--------------- 1257

  Fly  2248 FYANGHEADSQSQSERKSNYTSASCNEFVIACTYISGKTTWQNLDYVVRKTFKDYVARIDPGTNL 2312
                                ||.||               |:::|..:...|:.|::|||....|
 Worm  1258 --------------------TSQSC---------------WKDIDVSILGLFEVYLSRIDVEHQL 1287

  Fly  2313 GLNT-DSITSYHLGEAKR--GPEMGFPELLPCGYIVGSVRTLYICLQG-----VGSLAFDSLIPR 2369
            |::. |||..|.:||.:|  |.........|...:..|. |:.:.:.|     |.||..|.|:|:
 Worm  1288 GIDARDSILGYQIGELRRVIGDSTTMITSHPTDILTSST-TIRMFMHGAAQSRVDSLVLDMLLPK 1351

  Fly  2370 SIVHRYI-SLLTEHRRLILCGPSGTGKSYLARRLAEFLVARSARGNPSE-AIATFNVDHKSSKDL 2432
            .::.:.: |:||| |||:|.|.:|.|||.||:.||.::   |.|.|.|| :|...::...:.::|
 Worm  1352 QMILQLVKSILTE-RRLVLAGATGIGKSKLAKTLAAYV---SIRTNQSEDSIVNISIPENNKEEL 1412

  Fly  2433 RQYLGHIAEQAAIANGVSELPSVIILDNLHHASALGDVFSCLLSAGPANKLPCIIGTMSQ----- 2492
            .|....:.:...     |:...::||||:                 |.|::..::...:.
 Worm  1413 LQVERRLEKILR-----SKESCIVILDNI-----------------PKNRIAFVVSVFANVPLQN 1455

  Fly  2493 -----ATCNTTN------LQLHHNFRWVLTANHMEPVKGFLGRFLRRRLFQLE--LQTQHPQPEL 2544
                 ..| |.|      ||:||||:..:.:|.:|   ||:.|:||||..:.|  |..|.|. ||
 Worm  1456 NEGPFVVC-TVNRYQIPELQIHHNFKMSVMSNRLE---GFILRYLRRRAVEDEYRLTVQMPS-EL 1515

  Fly  2545 AAVLAWLPSVWQHINRFLEVHSSSDVTIGPRLFLACPMDLKDSQVWFTDIWNYHLSPYLVEAVRE 2609
            ..::.:.|...|.:|.|:|..:|.|||:|||..|.||:.:..|:.||..:||.:..|||....|:
 Worm  1516 FKIIDFFPIALQAVNNFIEKTNSVDVTVGPRACLNCPLTVDGSREWFIRLWNENFIPYLERVARD 1580

  Fly  2610 GVQLYGRRGGAWNDPSAFIRNSYPWPYGPDSVPPLRQINAEDV 2652
            |.:.:| |..::.||:..:...:||..|.:....|:::..:|:
 Worm  1581 GKKTFG-RCTSFEDPTDIVSEKWPWFDGENPENVLKRLQLQDL 1622

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
sick	NP_001260606.1	CH	535..637	CDD:214479	35/104 (34%)
		AAA	2382..2531	CDD:214640	46/165 (28%)
		AAA_22	2385..2476	CDD:290137	24/91 (26%)
unc-53	NP_001022170.1	CH	11..110	CDD:278723	33/101 (33%)
		DivIC	1148..1205	CDD:299713	19/91 (21%)
		AAA	1365..1471	CDD:214640	31/131 (24%)
		P-loop_NTPase	1367..1471	CDD:304359	29/129 (22%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	148	1.000	Domainoid score	I2763
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	305	1.000	Inparanoid score	I1596
Isobase	1	0.950	-	1	Normalized mean entropy	S5133
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001074
OrthoInspector	1	1.000	-	-		oto19106
orthoMCL	1	0.900	-	-		OOG6_103597
Panther	1	1.100	-	-	LDO	PTHR12784
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3248
SonicParanoid	1	1.000	-	-		X908
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	11	11.030