DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ref(2)P and Sqstm1

DIOPT Version :9

Sequence 1:	NP_001014491.1	Gene:	ref(2)P / 35246	FlyBaseID:	FBgn0003231	Length:	599	Species:	Drosophila melanogaster
Sequence 2:	NP_787037.2	Gene:	Sqstm1 / 113894	RGDID:	69287	Length:	439	Species:	Rattus norvegicus

Alignment Length:	513	Identity:	114/513 - (22%)
Similarity:	169/513 - (32%)	Gaps:	182/513 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly    93 VEEPKATKQEGSSANAEAPSVDDPSNFTIHDAVECDGCGLAPLIGFRYKCVQCSNYDLCQKCELA 157
            ::|.|..::|      ..|.....:...:|..|.||||. .|::|.||||..|.:||||..||..
  Rat    97 IKEKKECRRE------HRPPCAQEARSMVHPNVICDGCN-GPVVGTRYKCSVCPDYDLCSVCEGK 154

  Fly   158 HKHPEHLMLRMPTNNGPGMVDAWFTGPGLGRRSGRRSRGHCPFQETNQADPAGEPARDSRRERRQ 222
            ..|.||..|..|.                            ||...:      :....||..|:.
  Rat   155 GLHREHSKLIFPN----------------------------PFGHLS------DSFSHSRWLRKL 185

  Fly   223 ARRHAGVLTQFVEMMTNLPLNTTTATAPAEPQKPKAAEQTESPPQAEPTVTAEKAAESEAKPTEP 287
            ...|.|    :.......|.|.:       |:.|:|.:....|           .|||.:.|:|.
  Rat   186 KHGHFG----WPGWEMGPPGNWS-------PRPPRAGDGRPCP-----------TAESASAPSED 228

  Fly   288 KKVNTDQSVPRTEDPVTTPRSTQPTTPVINLDNISQIVPPEYMSAGIEILNNFSEMFSKIIDTTE 352
            ..||.                         |.|:.:.|.......|||:          .||...
  Rat   229 PNVNF-------------------------LKNVGESVAAALSPLGIEV----------DIDVEH 258

  Fly   353 GGDSGIFAPSTTPSAENKKPEEQGQSSGQSGASSANQSAVPSAAPSANQSNVPSANQSATPSISG 417
            ||......|:   |||:.....:.:|..|               ||:..|.|      :.|..:|
  Rat   259 GGKRSRLTPT---SAESSSTGTEDKSGTQ---------------PSSCSSEV------SKPDGAG 299

  Fly   418 SIPDAQLETEPLNPKPSETTTETEQERRRSDSL---DPEWQLIDNAYSANNSNLINLDTTNPTAA 479
            ..| ||..||.:.....|:..:.| |...||:.   |.:|                   |:.::.
  Rat   300 EGP-AQSLTEQMKKIALESVGQPE-ELMESDNCSGGDDDW-------------------THLSSK 343

  Fly   480 PQEPVRDFGQLGELLRQHMNEEARVEQASANTQTAQVDTVSTSTSTTSVTTNSVGTSPAAPDDKR 544
            ..:|     ..|||....|.|                          |...:|:..|...|...:
  Rat   344 EVDP-----STGELQSLQMPE--------------------------SEGPSSLDPSQEGPTGLK 377

  Fly   545 TVPVY-----HTDESINKSIHAMMAMGFSNEGAWLTQLLESVQGNISAALDVMNVSQN 597
            ...:|     ..|..:.:|:..|::||||:||.|||:||::...:|.||||.:..|::
  Rat   378 EAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKH 435

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ref(2)P	NP_001014491.1	PB1	6..88	CDD:295447
		ZZ	121..165	CDD:278966	22/43 (51%)
		UBA_SQSTM	555..593	CDD:270505	18/37 (49%)
Sqstm1	NP_787037.2	Interaction with LCK. /evidence=ECO:0000250\|UniProtKB:Q13501	2..48
		PB1_p62	4..100	CDD:99723	0/2 (0%)
		Interaction with PRKCZ and dimerization. /evidence=ECO:0000269\|PubMed:10477520, ECO:0000269\|PubMed:12431995	41..105		2/7 (29%)
		Interaction with PAWR. /evidence=ECO:0000250\|UniProtKB:Q13501	48..78
		Interaction with GABRR3. /evidence=ECO:0000269\|PubMed:12431995	119..221		38/158 (24%)
		ZZ_NBR1_like	123..163	CDD:239080	21/40 (53%)
		LIM protein-binding. /evidence=ECO:0000250\|UniProtKB:Q13501	167..217		12/94 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	201..231		10/47 (21%)
		TRAF6-binding. /evidence=ECO:0000250	225..230		2/4 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	259..389		38/205 (19%)
		Interaction with NTRK1. /evidence=ECO:0000269\|PubMed:11244088	266..439		56/246 (23%)
		MAP1LC3B-binding. /evidence=ECO:0000250\|UniProtKB:Q13501	320..341		7/40 (18%)
		LIR	335..340		2/23 (9%)
		Interaction with KEAP1. /evidence=ECO:0000250\|UniProtKB:Q64337	346..351		1/9 (11%)
		UBA_5	378..439	CDD:318725	21/58 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166337218
Domainoid	1	1.000	44	1.000	Domainoid score	I12039
eggNOG	1	0.900	-	-		E1_KOG4582
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	60	1.000	Inparanoid score	I5306
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1275680at2759
OrthoFinder	1	1.000	-	-		FOG0002104
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR15090
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X5995
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	8	7.990