Protein Alignment Hasp and Svep1

DIOPT Version :10

• Sequence 1: NP_001137842.2
• Gene: Hasp / 35238
• FlyBaseID: FBgn0032797
• Length: 1677
• Species: Drosophila melanogaster
• Sequence 2: NP_073725.2
• Gene: Svep1 / 64817
• MGIID: 1928849
• Length: 3567
• Species: Mus musculus
• Alignment Length: 1944
• Identity: 427/1944 - (21%)
• Similarity: 647/1944 - (33%)
• Gaps: 557/1944 - (28%)

  Fly    59 GRVYKNPRNSPSTECPRDEEQATLLGQKCLRKCSSDEDCKSKKKKCLCD---GVCGNS---CI-- 115
            |:|..:..:...::||..|...     ..||..|.:.. ...|....||   .:.||.   |:
Mouse  1616 GKVKVDSSSMFCSDCPSLEGSV-----PHLRPASGNRK-PGSKVSLFCDPGFQMVGNPVQYCLNQ 1674

  Fly   116 ----KPDRECPEL---AQPSL--GQVTVAGRHFGARASYACPHGYHVVGLQSRLCQADGNWAGAE 171
                :|...|..:   ..|:|  |..:....|.|:..:|.|..||:::|.....|..:|:|.|..
Mouse  1675 GQWTQPLPHCERIRCGLPPALENGFYSAEDFHAGSTVTYQCTSGYYLLGDSRMFCTDNGSWNGIS 1739

  Fly   172 PA------------CKQNIYCL----------KPPQIEHARNSALPEQ--------------ETF 200
            |:            |.::..||          .||.....:|.|.|.:              |.:
Mouse  1740 PSCLDVDECAVGSDCSEHASCLNTNGSYVCSCNPPYTGDGKNCAEPVKCKAPENPENGHSSGEIY 1804

  Fly   201 DLDSTVQYHCHTGYVTNGFPRAKCLAIDNLASWYGPDIQCEPRSCGQPPDPAYGWHAGECYTYGC 265
            .:.:.|.:.|..|:...|.....||   ....|......||..|||.||.|..|...|..:|||.
Mouse  1805 TVGTAVTFSCDEGHELVGVSTITCL---ETGEWDRLRPSCEAISCGVPPVPENGGVDGSAFTYGS 1866

  Fly   266 KITYNCGTGYELVGKHERYCQSDGSWTPKELPTCVLVTSVVCPTPENPKNGKATYTTLAYNSVVS 330
            |:.|.|..||.|.|..|..|.:.|||:... |.|.||.   |..||:..|||...:.|.|.|:.|
Mouse  1867 KVVYRCDKGYTLSGDEESACLASGSWSHSS-PVCELVK---CSQPEDINNGKYILSGLTYLSIAS 1927

  Fly   331 YECRYGYTLVGESSSRCGADRKWSGTLPTCKEINCGHPGVLYNGWIENIEAGTG----LGASIIF 391
            |.|..||:|.|.|...|.|...|....|:|:.::||.|.::.:..|      ||    .|.::.:
Mouse  1928 YSCENGYSLQGPSLLECTASGSWDRAPPSCQLVSCGEPPIVKDAVI------TGSNFTFGNTVAY 1986

  Fly   392 RCQPEMMINGLGSSVCQIDGRWRNALPECLA-PCVVPTISQGNV--------------------- 434
            .|:....:.|..:.|||.:|:|.::..:||| .|..|.    ||
Mouse  1987 TCKEGYTLAGPDTIVCQANGKWNSSNHQCLAVSCDEPP----NVDHASPETAHRLFGDTAFYYCA 2047

  Fly   435 --YPI----EIITDENGTTIVHPPTTTT----------QSPTQTQSI-GGVEKVKH--GTALEVK 480
              |.:    ::|.:..|..:  ||....          :.|:.:.|| ..|.|.|.  |:.:..|
Mouse  2048 DGYSLADNSQLICNAQGNWV--PPAGQAVPRCIAHFCEKPPSVSYSILESVSKAKFAAGSVVSFK 2110

  Fly   481 CDENYEFPVSLLSPPTC-NNGTWSIIP---RCVPARCKSMPRPPK--HGMVMAPKTEHGMKARFK 539
            |.|.  |.::..:...| ..|.||..|   :|:|.||   ..||.  :|.........|....:.
Mouse  2111 CMEG--FVLNTSAKIECLRGGEWSPSPLSVQCIPVRC---GEPPSIANGYPSGTNYSFGAVVAYS 2170

  Fly   540 CKDGFKLVSPEGKDVTDPHDYVLTC-SFGNWTGETPKCDEVFCSFPGYIPNGKVLLVGNMGLYDY 603
            |..|| .:..|.|.         || :.|.|:..||.|..|.|:.|..:.||            :
Mouse  2171 CHKGF-YIKGEKKS---------TCEATGQWSKPTPTCHPVSCNEPPKVENG------------F 2213

  Fly   604 RPYVKKIVNNKQIMYDCDKGYVLEVGPPGATCVGGK-WR---PLDLPQCLLGQHPRLRWNRRRRR 664
            ..:........:..:.|:.|| ...|.|...|...: |.   ||.......|:.|.::....:..
Mouse  2214 LEHTTGRTFESEARFQCNPGY-KAAGSPVFVCQANRHWHSDAPLSCTPLNCGKPPPIQNGFLKGE 2277

  Fly   665 SLQIRQLRSMYLLQRQRQLQRQLIDNQNYIKAIADPVQPSVHRVKRSASPHPNSQPYASDVDRAY 729
            |.::..             :.|.:.|:.| :.:.|.........|.|..|.|...|
Mouse  2278 SFEVGS-------------KVQFVCNEGY-ELVGDNSWTCQKSGKWSKKPSPKCVP--------- 2319

  Fly   730 SKYYQKIKERYQRYVRKMLGRDRIYQRLHSQDAVGRVGNNLNTHDGGQVTMRGKSNFREFENGNN 794
                .|..|      ..:|....:.:.|.|:..|    ..::..:|.  .::|.|..:...:| .
Mouse  2320 ----TKCAE------PPLLENQLVLKELASEVGV----MTISCKEGH--ALQGPSVLKCLPSG-Q 2367

  Fly   795 YLGSGGIGSGGVVGSGVVALPNINQASRNHQVHVNRTPKEELLSNGSPPI-------ASRSLHLN 852
            :.||..|       ..:|..|                         |||:       :|.:||..
Mouse  2368 WNGSFPI-------CKMVLCP-------------------------SPPLIPFGVPASSGALHFG 2400

  Fly   853 ATRSYIEQLKSQIVRRRRR---RSSSIGQSPPPSGATI--PDAEVDISDG--------------- 897
            :|..|: .:....:|....   ::.|...||.|....:  |..| :|.:|
Mouse  2401 STVKYL-CVDGFFLRGSPTILCQADSTWSSPLPECVPVECPQPE-EILNGIIHVQGLAYLSTTLY 2463

  Fly   898 ----------------GEAGKGGGGK--------------------------------------R 908
                            ||.|:..|||                                      |
Mouse  2464 TCKPGFELVGNATTLCGENGQWLGGKPMCKPIECPEPKEILNGQFSSVSFQYGQTITYFCDRGFR 2528

  Fly   909 LRGP----CEEL-EWD---------SFANVTTVRPGKVPGRN-AVGIMLHLECNAGFKLNIKGEN 958
            |.||    |.|. :||         ..::...:..|.|.|.: ..|.|:...|..||:  :.| :
Mouse  2529 LEGPKSLTCLETGDWDMDPPSCDAIHCSDPQPIENGFVEGADYRYGAMIIYSCFPGFQ--VLG-H 2590

  Fly   959 ATARCIRGIWKPETPKCLSAPC-LVPAVEHGQYYKV---EPHTKQLSDK---PSL---------- 1006
            |...|....|...:|.|:...| |.|.::.|...||   :.|..|..|.   |.|
Mouse  2591 AMQTCEESGWSSSSPTCVPIDCGLPPHIDFGDCTKVRDGQGHFDQEDDMMEVPYLAHPQHLEATA 2655

  Fly  1007 --------TPLSTYEEIQSNEFITLECEDGFNIQGSAQLRCAH-GSWSVNAFSECTSVPCTLPNI 1062
                    :|.|..........::..||.|:.:.|...|.|.. |:|:..|.| |.|:.|.||..
Mouse  2656 KALENTKESPASHASHFLYGTMVSYSCEPGYELLGIPVLICQEDGTWNGTAPS-CISIECDLPVA 2719

  Fly  1063 ----------------------PGVIYDGGY-RAGLTIGHGSSVSVRCEL--STNANPIEMSCHK 1102
                                  ||.|.:|.: |..|.....|....|||.  .:..||:.....|
Mouse  2720 PENGFLHFTQTTMGSAAQYSCKPGHILEGSHLRLCLQNKQWSGTVPRCEAISCSKPNPLWNGSIK 2784

  Fly  1103 G----ILTPPSIPCESG--LRKSREELEHATPTPHPKVEHNELDNDHDHHDNGTDEHDDMKMC-- 1159
            |    .|......|:||  |..|::.          ..:.|   .|.|.|:         .||
Mouse  2785 GDDYSYLGVLYYECDSGYILNGSKKR----------TCQEN---RDWDGHE---------PMCIP 2827

  Fly  1160 ----GPPMLTDGALVYKNADHPELDGAYESGTEIFFNCIPNAAGDRQTWRIICDNGLWIGRSYNC 1220
                .||:.|:|.:  |..:       |....||.::|......:....||...||.|.|.:.:|
Mouse  2828 VDCGSPPVPTNGRV--KGEE-------YTFQKEITYSCREGFILEGARSRICLTNGSWSGATPSC 2883

  Fly  1221 ENGTCVFRNNEANVVSFYNDLEIREDVVDFPPGATIISRCMDIGKFSFMGSHERTC-IHSEWTNT 1284
            ....|.......|.|:         |.:|:.....:...|::  .:...|:...|| .:..|...
Mouse  2884 MPVRCPAPPQVPNGVA---------DGLDYGFKKEVAFHCLE--GYVLQGAPRLTCQSNGTWDAE 2937

  Fly  1285 KPVCSGLNQENDYAMEKAPTILFRHQNGPIAQSNDG---KLIVYPGTTLHMECL-WMRRFGNP-- 1343
            .|||            |..|.      ||.|....|   ....|.|..:..:|. ..:..|||
Mouse  2938 VPVC------------KPATC------GPPADLPQGFPNGFSFYHGGHIQYQCFTGYKLHGNPSR 2984

  Fly  1344 ------KWNVSH--------THKNYTEGWVTEADEGRDSTLEYR-------LSIVDAASDDSGFY 1387
                  .|:.|.        :.....:|.:...|.|...|::..       |.:.:...|.:|.:
Mouse  2985 RCLPNGSWSGSSPSCLPCRCSTPIIQQGTINATDLGCGKTVQIECFKGFKLLGLSEITCDANGQW 3049

  Fly  1388 SCMTPARHEHTVEVVVRAINCPEIPMRRGLIVNTNDTKLSTRALLSCANGNSLIGASELFCLPSG 1452
            |.:....|          ..|..:|.....||.............||..|.::.|:|:|.|....
Mouse  3050 SDVPLCEH----------AQCGPLPTIPNAIVLEGSLSEDNVVTYSCRPGYTMQGSSDLICTEKA 3104

  Fly  1453 NWSAPLPVCESVECGDIPLGMGSNASS-------------------------------------- 1479
            .||.|.|.||.:.||..|....:.|:.
Mouse  3105 IWSQPYPTCEPLSCGPPPTVANAVATGEAHTYESKVKLRCLEGYVMDSDTDTFTCQQDGHWVPER 3169

  Fly  1480 --------PRVSVLSR--------EVGGRAAFSCASGYGLRGPAEAICNPTGEWSAPL--PTCVE 1526
                    |..|.::|        :|..:.:.|||.|:...|...:.|.|.|.|..|.  .:|:.
Mouse  3170 ITCSPKKCPVPSNMTRIRFHGDDFQVNRQVSVSCAEGFTHEGVNWSTCQPDGTWEPPFSDESCIP 3234

  Fly  1527 VQCDNPGAPQNGYAQGSAPYRAGDVVQFNCNPEYMMQGQPIIACQDNARWSGGLPKCVQ-ACSYP 1590
            |.|.:|.:|.:|...|: .:..|..:.:.|:|.|.::|.....||:|.:|||.:..|.: .|..|
Mouse  3235 VVCGHPESPAHGSVVGN-KHSFGSTIVYQCDPGYKLEGNRERICQENRQWSGEVAVCRENRCETP 3298

  Fly  1591 GTVISGRMSSVKFYYAIGESITFTCDAGLDLRGSKVLKCLKNGKWSSAIPTCVTNEGPV 1649
            ....:|:  :|......|.|:.|:|..|..|.||....|..||.|:...|.|..|..||
Mouse  3299 AEFPNGK--AVLENTTSGPSLLFSCHRGYTLEGSPEAHCTANGTWNHLTPLCKPNPCPV 3355

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hasp	NP_001137842.2	CCP	121..175	CDD:153056	16/70 (23%)
		PHA02927	182..421	CDD:222943	78/256 (30%)
		PHA02927	<497..>654	CDD:222943	40/167 (24%)
		PHA02927	<946..1111	CDD:222943	52/219 (24%)
		CCP	1408..1462	CDD:153056	15/53 (28%)
		PHA02927	<1488..1643	CDD:222943	47/157 (30%)
Svep1	NP_073725.2	VWA	84..259	CDD:459670
		Ephrin_rec_like	311..361	CDD:429604
		CCP	379..435	CDD:153056
		CCP	439..495	CDD:153056
		CCP	<514..556	CDD:153056
		HYR	560..642	CDD:460572
		HYR	644..722	CDD:460572
		Ephrin_rec_like	1004..1051	CDD:429604
		Ephrin_rec_like	1058..1105	CDD:429604
		Ephrin_rec_like	1112..1159	CDD:429604
		EGF_CA	1195..1228	CDD:238011
		EGF_CA	1230..1266	CDD:238011
		EGF_CA	1268..1304	CDD:238011
		EGF_CA	1306..1342	CDD:238011
		EGF_CA	1344..1380	CDD:238011
		EGF_CA	1382..1418	CDD:238011
		PTX	1423..1621	CDD:238086	2/4 (50%)
		PHA02927	1643..1899	CDD:222943	66/260 (25%)
		EGF_3	1748..1782	CDD:463759	5/33 (15%)
		PHA02817	1899..>2008	CDD:165167	38/117 (32%)
		PHA02927	1957..2198	CDD:222943	62/267 (23%)
		PHA02927	2093..2318	CDD:222943	58/266 (22%)
		PHA02831	2277..>2435	CDD:165176	38/230 (17%)
		PHA02927	2400..2608	CDD:222943	38/212 (18%)
		Important for the interaction with integrin ITGA9:ITGB1. /evidence=ECO:0000269|PubMed:22654117	2638..2645		2/6 (33%)
		CCP	<2670..2710	CDD:153056	10/40 (25%)
		PHA02927	2709..2941	CDD:222943	58/273 (21%)
		PHA02927	2903..3113	CDD:222943	47/239 (20%)
		PHA02927	3012..3225	CDD:222943	43/222 (19%)
		PHA02927	3232..3464	CDD:222943	40/127 (31%)