Protein Alignment cyst and ROM1

DIOPT Version :10

• Sequence 1: NP_001260588.1
• Gene: cyst / 35237
• FlyBaseID: FBgn0032796
• Length: 1319
• Species: Drosophila melanogaster
• Sequence 2: NP_011584.2
• Gene: ROM1 / 852961
• SGDID: S000003302
• Length: 1155
• Species: Saccharomyces cerevisiae
• Alignment Length: 1189
• Identity: 234/1189 - (19%)
• Similarity: 410/1189 - (34%)
• Gaps: 376/1189 - (31%)

  Fly    58 ARAPIAPPPAS-----NPASNTINNNNMIPTINVTPHSPALASKYNNIFEDTLSQLQNIRETVVQ 117
            ||..|||..:|     :|..:...|.|..|. :.|....:..:|..:|.:.|.....|...|
Yeast    74 ARYRIAPDCSSHQLKASPVLHISTNLNSSPQ-SFTGDQISPTNKKISINDSTRQDKGNSCTT--- 134

  Fly   118 MKNSSSPSQHMQDGSLAN---HGLLSAAILSTSL--------PDLTGTGTGPASGGSNYAMWSST 171
               :|||||...:..|.:   |.  |.::||.|.        |:...|...|.|.|....:.||.
Yeast   135 ---TSSPSQKRSNVLLPHVRKHS--SPSLLSFSKNSGSHMGDPNQLSTPPTPKSAGHTMELHSSF 194

  Fly   172 APQQCQFLHTDRRKSWTAVDDGSGAGDCTNKSVSLSSLDSEEQETIRVTEQRRRSARNSTGGIST 236
                                :|..:...|:...:|.||.:          |.|||: ||:...|.
Yeast   195 --------------------NGKHSSSSTSSLFALESLKT----------QNRRSS-NSSNHSSQ 228

  Fly   237 HSLNEAELARDFERIAAKRSLATEI--ISRIPL--QKSISTSSIIAKEDIKAIARH--------- 288
            :..:..:..|...|..:.....|||  |...||  ...:|..:|..|:.|| ::.|
Yeast   229 YRRHTNQHQRHHSRSKSSPVSLTEISMIKGTPLVYPALLSLIAIKFKQTIK-LSTHKKMGLLYRD 292

  Fly   289 -------------LTDSEDENQSLLRA-------------------HAKIEVYDTVEKRPKRGSI 321
                         :..|.|.|..:|..                   .:.:|:|:...:     ||
Yeast   293 SFTGKQAIDTLCLIIGSLDRNLGMLIGKSLEAQKLFHDVLYDHGVRDSVLEIYELSSE-----SI 352

  Fly   322 FFRKKKPK--------AKTKSLVGGQLSACDVCGAAITLAQIKEHHLECKVKKMGGGQDYYDGPD 378
            |...:...        :.:.|.|....:..::.|..:.|...  :...|.::|:
Yeast   353 FMAHQSQSSTSIANTFSSSSSSVNSLRTKTEIYGVFVPLTHC--YSSTCSLEKL----------- 404

  Fly   379 ASTFSNPEDQPLIRSDLQFLNEAPIEAQDLGADPVLGIVL---KEHD---SWTPNVPRELLKTLK 437
            ..:.|.|.         :...:|.:..: ||......|.|   ||.|   |||.:||:.:.::|.
Yeast   405 CYSISCPN---------RLQQQANLHLK-LGGGLKRNISLALDKEDDERISWTNSVPKSVWESLS 459

  Fly   438 DLQIKRQEHIYEFIMTEKHHCQTLIVMQKVFVESL--ERHFPS---LN-LNSMFPRLQQLTDLHT 496
            ..||||||.|||...|||...::|.:::..|::.|  ....||   :| :..:|..:.::..::.
Yeast   460 KQQIKRQEAIYELFTTEKKFVKSLEIIRDTFMKKLLETNIIPSDVRINFVKHVFAHINEIYSVNR 524

  Fly   497 CFLRQLRLKQREQHMVDSIADILLD---FFSLEQAQCLRLAYGEFCANHRSALDQFKLCLTEPSF 558
            .||:.|..:|....:...||||.|.   ||....:......|.::....:.:::        |:|
Yeast   525 EFLKALAQRQSLSPICPGIADIFLQYLPFFDPFLSYIASRPYAKYLIETQRSVN--------PNF 581

  Fly   559 AEWYKHCLQNPLLKKKGIPECILFVTQRLTKYPLLIEPLLKSARDNKLETDKLQHAL---NLVKS 620
            |.:......:.|  :.||...:.....|..:|.||:..::..: |...:.|.||..:   :|:|.
Yeast   582 ARFDDEVSNSSL--RHGIDSFLSQGVSRPGRYSLLVREIIHFS-DPVTDKDDLQMLMKVQDLLKD 643

  Fly   621 LLVDVDACVAEKELRERQLEIYGRVDAKSFAIYKNKPFRKTE---LGVES-RRRLKFDGLATLMQ 681
            |:..:|......:         .|.|.|   :.|.|...|.|   ||:.: :|::|.:||.: .:
Yeast   644 LMKRIDRASGAAQ---------DRYDVK---VLKQKILFKNEYVNLGLNNEKRKIKHEGLLS-RK 695

  Fly   682 GRAKTQL-----VLVVVLTDCLCFLSE---NSGHNKYSFFTPEHKAGVVPLQKLLI--------R 730
            ...||..     :...:|.:.|.||..   |..|....|..|      :||..|.|        .
Yeast   696 DVNKTDASFSGDIQFYLLDNMLLFLKSKAVNKWHQHTVFQRP------IPLPLLFICPAEDMPPI 754

  Fly   731 EKAGTE----SRGIYI---ISSNP---------DFPEMYELKVQTPKDKNTWIQTIRQAVLDCPA 779
            ::..||    |.|:.:   .:|||         ...:.|::.:..|:...  :||:
Yeast   755 KRYVTENPNCSAGVLLPQYQTSNPKNAIVFAYYGTKQQYQVTLYAPQPAG--LQTL--------- 808

  Fly   780 TDIIEAEDLTAEEKLRIGVNKRETIEKMRQKDIEQALLLEEKLMLQFNLLKEQQPFGDVAGTNS- 843
                                    |||::|   ||..||:|...:.|..:..|. |.....||.
Yeast   809 ------------------------IEKVKQ---EQKRLLDETKHITFKQMVGQF-FHSYINTNRV 845

  Fly   844 ----SCSAANFLAAFGSYKDLVSADCDTAELWRRVLNTVQDISQLASTIYSAATGQPV----PRT 900
                .|.|...|        ||:.:     :...|||....|:|           :||    ..:
Yeast   846 NDVLICHAGKIL--------LVATN-----MGLFVLNYATSINQ-----------KPVHLLHKIS 886

  Fly   901 LSSVGEKQSEQYASPTLPKR-----------AETFAGFDEKRGKLASK----------------- 937
            :|.:...:..:.....:.|:           ||.......|..|:..|
Yeast   887 ISQISVLEEYKVMILLIDKKLYGCPLDVIDDAENADFLFRKNSKVLFKYVAMFKDGFCNGKRIIM 951

  Fly   938 -----------LVLTPRLQELEE---KREPKSG--SSSVSEQPLENNYAVLQVTHHLQTLLCV-- 984
                       |::.|.:.:...   |:..|:|  ..||..:||..::        |:..:|:
Yeast   952 IAHHFLHAVQLLIVNPLIFDFNSGNFKKNLKAGLVDFSVDSEPLSFSF--------LENKICIGC 1008

  Fly   985 ------------------ISQQMTTINNLQLQLALYRES-------P-RSSAYTHKDQLEELRNL 1023
                              ..:::..:::.::...:|:|:       | ::|.:....:|....|.
Yeast  1009 KKNIKILNVPEVCDKNGFKMRELLNLHDNKVLANMYKETFKVVSMFPIKNSTFACFPELCFFLNK 1073

  Fly  1024 QDKQQEEKTA--WQRQLKQ 1040
            |.|::|.|..  |:.:.:|
Yeast  1074 QGKREETKGCFHWEGEPEQ 1092

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
cyst	NP_001260588.1	RhoGEF	447..627	CDD:214619	42/191 (22%)
		PH_ARHGEF2_18_like	671..774	CDD:275432	28/134 (21%)
		SMC_prok_B	935..>1073	CDD:274008	24/169 (14%)
ROM1	NP_011584.2	ROM1	1..1155	CDD:227709	234/1189 (20%)