DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top3alpha and TOP3B

DIOPT Version :9

Sequence 1:	NP_523602.2	Gene:	Top3alpha / 35236	FlyBaseID:	FBgn0040268	Length:	1250	Species:	Drosophila melanogaster
Sequence 2:	NP_001269041.1	Gene:	TOP3B / 8940	HGNCID:	11993	Length:	862	Species:	Homo sapiens

Alignment Length:	947	Identity:	277/947 - (29%)
Similarity:	409/947 - (43%)	Gaps:	203/947 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly    29 LNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQNAKMVMTSVSGHMMQLAFQVS 93
            |.||||...|::||.:||.|:....:|.:....|.::.....||..:..||||.||:|.|.|...
Human     5 LMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGK 69

  Fly    94 YKNWRTVDPRSLF-DAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEIIDVCR 157
            |..|..|||..|| .||.:|...:....:.:.|:.|.|||..:::|.|||:|||||.:|::|
Human    70 YNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLD--- 131

  Fly   158 AIKPNI--------SVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQ 214
            |:.|.:        :|:||.||.||...:..|:..||:||..::.:||.|.|||||.|.|.||||
Human   132 AVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQ 196

  Fly   215 TMRLQRLFPEKIAD---KLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKV-LHTIDDLTVEF 275
            |    :.|..|..|   .|||:|.||.|||||..||:.:|::|..|.:|.::. ::|..|.::..
Human   197 T----KYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLL 257

  Fly   276 NWARNRLFDKEACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKE 340
            :|.|.|:||:|..:.:|.:...|.:  |.||:.:.|.|.|.||..|:||||.::.|..|.:..:.
Human   258 DWDRVRVFDREIAQMFLNMTKLEKE--AQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQH 320

  Fly   341 TMTIAEKLYTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIEWGPN-PRNGNKSD 404
            .|..||:|||:|:||||||||..:.:.|.|...:..|..|..|....:|::..|.| ||.|:.:.
Human   321 AMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAG 385

  Fly   405 QAHPPIHPTKLA--ENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANGLVIHE 467
            . ||||.|.|.|  ..|.|:..|:||.:.|||:|.||.|....::.:...|..|.||.:|..:..
Human   386 D-HPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLS 449

  Fly   468 RNYLDVYVYDKWSA----KQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTD 528
            ..:.:|.   .|.:    :.:...:.|..|...||.:.|..|..|..||||:||.||||||||||
Human   450 PGFTEVM---PWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTD 511

  Fly   529 ATHAEHINTIKERGYIGVLDKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQK 593
            |:...|||.|.:|.|:.|.....|.|..:|:.|..||..::..|..|.:|:..|..|.||.||:.
Human   512 ASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKA 576

  Fly   594 DPKVVLTEQIAKYKQAYQQITDKITAMDAKISARINETPAANSAVQEGADG-------------- 644
            |.:.||...:..:|:.:....|.|..||..:  .::.:|.|.:.......|
Human   577 DYRQVLGHTLDVFKRKFHYFVDSIAGMDELM--EVSFSPLAATGKPLSRCGKCHRFMKYIQAKPS 639

  Fly   645 -----------SAPSHGII------------------------QSIFQCPKCNEAPLALKPKKNQ 674
                       :.|.:|.|                        :|...||.|...|.....||.
Human   640 RLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKG- 703

  Fly   675 QGWYIGCN--NFPDCKNAVWL-----------------PTE-------CKDASVLDEC------- 706
                :|||  ..|.|::::.:                 ||.       |...:|:..|
Human   704 ----MGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRV 764

  Fly   707 ------CPTCGDGYRMLKFRLSTPYYRGVFGTPSGWYKTCLPCDNLFRTTFNINLDSVKKVGGIV 765
                  |..|......:.|..:.....|    ....:..|:.||.:|:     .|..:|......
Human   765 RVSADTCSVCEAALLDVDFNKAKSPLPG----DETQHMGCVFCDPVFQ-----ELVELKHAASCH 820

  Fly   766 GEVRGGGGGPGPGPGGGGSGRGAGSGGWSSGPGGGGSGGGGGSGGWGSGTGGGGSGGWGSGTGGG 830
            ...|||        .|...|||.|
Human   821 PMHRGG--------PGRRQGRGRG----------------------------------------- 836

  Fly   831 GLGGGKGKKPGGESKKSATKKPPNEPKPKKTKEPKAA 867
                             ..::||.:|.|::.|:..:|
Human   837 -----------------RARRPPGKPNPRRPKDKMSA 856

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TopA	27..618	CDD:223624	227/608 (37%)
		TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	60/164 (37%)
		TOP1Ac	194..608	CDD:238110	161/424 (38%)
		zf-C4_Topoisom	657..694	CDD:279704	11/55 (20%)
		YrdD	658..>744	CDD:223625	21/124 (17%)
		zf-GRF	1033..1076	CDD:284302
		zf-GRF	1150..1193	CDD:284302
TOP3B	NP_001269041.1	TOPRIM_TopoIA_TopoIII	3..167	CDD:173782	60/164 (37%)
		TOP1Ac	172..593	CDD:238110	162/430 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	821..854		13/98 (13%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0550
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG62408
OrthoDB	1	1.010	-	-		D104272at33208
OrthoFinder	1	1.000	-	-		FOG0000966
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	5	4.830