DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top3alpha and TOP3A

DIOPT Version :9

Sequence 1:	NP_523602.2	Gene:	Top3alpha / 35236	FlyBaseID:	FBgn0040268	Length:	1250	Species:	Drosophila melanogaster
Sequence 2:	NP_201197.1	Gene:	TOP3A / 836513	AraportID:	AT5G63920	Length:	926	Species:	Arabidopsis thaliana

Alignment Length:	982	Identity:	366/982 - (37%)
Similarity:	498/982 - (50%)	Gaps:	172/982 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    23 GQAMKYLNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQNAKMVMTSVSGHMMQ 87
            |..:..||||||...||::||:||.|..:.|||.|.|||:|:|:..:.||..:|:||||.||:|:
plant     6 GGPVTVLNVAEKPSVAKSVAGILSRGTFRTREGRSRYNKIFEFDYAINGQPCRMLMTSVIGHLME 70

  Fly    88 LAFQVSYKNWRTVDPRSLFDAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEI 152
            |.|...|:.|.:.||..|:.|||.|.|..|.:.||:|||.|.|....|::|.|||||||||.:|:
plant    71 LEFADRYRKWHSCDPADLYQAPVMKHVPEDKKDIKKTLEEEARKSDWLVLWLDCDREGENIAFEV 135

  Fly   153 IDVCRAIKPNISVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMR 217
            :|||||:|.|:.:.||.||.:....:..|:|.|..|::..::|||.|.|:|||.||:.||||||.
plant   136 VDVCRAVKHNLFIRRAHFSALIDRDIHEAVQNLRDPNQLFAEAVDARQEIDLRIGASFTRFQTML 200

  Fly   218 LQRLF------PEKIADKLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKVLHTIDDLTVEFN 276
            |:..|      .|:  .::||||.||.|||||:.|||.||:|...|.||.|...|..::....||
plant   201 LRDRFAIDSTGEER--SRVISYGPCQFPTLGFIVERYWEIQAHEPEEFWTINCSHQSEEGLATFN 263

  Fly   277 WARNRLFDKEACENYLLLCLAEPDPRALVESVT-VKPKHKWRPTPLDTVEMEKLGSRKLKLSAKE 340
            |.|..|||..:......:|:.|  |.|.|.:|. .:.:.|:.|.||:|:|:||..||..:||::.
plant   264 WMRGHLFDYASAVILYEMCVEE--PTATVMNVPHPRERFKYPPYPLNTIELEKRASRYFRLSSEH 326

  Fly   341 TMTIAEKLYTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIE-----WGPNPRNG 400
            ||.:||:||..||||||||||:.||....|..:||.||.|..||::|||::|     | .||.||
plant   327 TMKVAEELYQAGFISYPRTETDSFSSRTDLRAMVEEQTRHPAWGSYAQRLLEPEGGLW-RNPANG 390

  Fly   401 NKSDQAHPPIHPTKLA---ENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANG 462
            ...|:|||||||||.:   .|...:...||||||||:|||||:.||.:||.|.||||||:|:|:|
plant   391 GHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVRHYLACVSQPAVAAETTVEIDIAGERFSASG 455

  Fly   463 LVIHERNYLDVYVYDKWSAKQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGT 527
            ..|..:|||:||.::.|....|..||.||:|.||.::|....|..||||.||||::.|:|.||||
plant   456 RAILAKNYLEVYRFESWGGSVIPVYEKGQQFIPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGT 520

  Fly   528 DATHAEHINTIKERGYIGVLDKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQ 592
            |||..:||..:.:|||..........|..:|..|..|||.|...|.||.|||..|.|:..:..|:
plant   521 DATMHDHIKKLLDRGYATKDANTRFSPTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGR 585

  Fly   593 KDPKVVLTEQIAKYKQAYQQITDKITAMDAKIS------------ARINETPAANSAVQEGADGS 645
            |....||...:.:.|..:         :||::.            .|.|.|..:.|....
plant   586 KTKAEVLETCLQQMKACF---------LDARVKKSKLLEAMTIFFERSNNTDESESQTAG----- 636

  Fly   646 APSHGIIQSIFQCPKCNEAPLALKPKKNQQG-WYIGCNNFPDCKNAVWLPTECKDASVLDECCPT 709
                   :.:.:|..|||:.:||  :||:.| :.:||.|:|.|:||||||....:|||....|.:
plant   637 -------EVVRRCNLCNESDMAL--RKNRDGNFMVGCMNYPQCRNAVWLPGPTLEASVTTNVCQS 692

  Fly   710 CGDG--YRML-KFRLSTPYYRGVFGTPSGW---YKTCL-PCDNLFRTTFNI----NLDSVKKVGG 763
            ||.|  |::| |||        ..|.|.|:   :..|: .||::.:...:|    :....::..|
plant   693 CGPGPVYKILFKFR--------QIGIPPGFDVNHLGCVGGCDDILKQLIDICGTGSRSQARRTPG 749

  Fly   764 IV--GEVRGGG------------------GGPGPGPGGGGS------------------------ 784
            ..  ..::|..                  ..|...|....|
plant   750 TAPSNNIQGSNTRQSNVCIHCQQRGHASTNCPSRVPASRNSRPTATNPRNDESTVSCNTCGSQCV 814

  Fly   785 ----------GR--------GAGSGGW------SSGPGGGGSGGGGGSGGWGS-GTGGGGSGGWG 824
                      ||        |.....|      |||....|| ..||||..|| |.|.||.||..
plant   815 LRTANTEANRGRQFFSCPTQGCSFFAWEDSINNSSGNATTGS-NSGGSGRRGSRGRGRGGRGGQS 878

  Fly   825 SGTGGGGLGGGKGKKPGGESKKSATKKPPNEPKPKKTKEPKAAPNKKTSSKSSGSIRSFFTSAAP 889
            ||          |::..|.|..|||.:|.:..:.....:|                 |.|.:|.|
plant   879 SG----------GRRGSGTSFVSATGEPVSGIRCFSCGDP-----------------SHFANACP 916

  Fly   890 TNSASNG 896
            ..:.|||
plant   917 NRNNSNG 923

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TopA	27..618	CDD:223624	277/605 (46%)
		TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	77/157 (49%)
		TOP1Ac	194..608	CDD:238110	197/428 (46%)
		zf-C4_Topoisom	657..694	CDD:279704	18/37 (49%)
		YrdD	658..>744	CDD:223625	36/93 (39%)
		zf-GRF	1033..1076	CDD:284302
		zf-GRF	1150..1193	CDD:284302
TOP3A	NP_201197.1	PRK07219	10..702	CDD:235971	311/719 (43%)
TOP3A	NP_201197.1	zf-GRF	804..844	CDD:284302	4/39 (10%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	348	1.000	Domainoid score	I225
eggNOG	1	0.900	-	-		E1_COG0550
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H3394
Inparanoid	1	1.050	617	1.000	Inparanoid score	I213
OMA	1	1.010	-	-		QHG62408
OrthoDB	1	1.010	-	-		D373433at2759
OrthoFinder	1	1.000	-	-		FOG0000966
OrthoInspector	1	1.000	-	-		oto3224
orthoMCL	1	0.900	-	-		OOG6_101027
Panther	1	1.100	-	-	LDO	PTHR11390
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3214
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.840