DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Top3alpha and top3b

DIOPT Version :9

Sequence 1:	NP_523602.2	Gene:	Top3alpha / 35236	FlyBaseID:	FBgn0040268	Length:	1250	Species:	Drosophila melanogaster
Sequence 2:	XP_003198651.1	Gene:	top3b / 793414	ZFINID:	ZDB-GENE-070705-37	Length:	861	Species:	Danio rerio

Alignment Length:	864	Identity:	279/864 - (32%)
Similarity:	414/864 - (47%)	Gaps:	131/864 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    29 LNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQNAKMVMTSVSGHMMQLAFQVS 93
            |.||||...|::||.:||.|:...|:|.:....|.::.....|||.:..||||.||:|.|.|...
Zfish     5 LMVAEKPSLAQSIAKILSKGSCSTRKGLNGACSVHEYMGSFTGQNVRFKMTSVCGHVMSLDFIGK 69

  Fly    94 YKNWRTVDPRSLFD-APVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEIIDVCR 157
            |.||..|||..||. ||.:|...:....:.:.|:.|.:||..:::|.|||:|||||.:|::|..:
Zfish    70 YNNWDKVDPAELFSKAPTEKKEANPKLNMVKFLQVEAKGCDYVVLWLDCDKEGENICFEVLDAIQ 134

  Fly   158 AI--KP---NISVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMR 217
            .:  ||   ..:||||.||.||...:..|:.:||:|:|.::.:||.|.|||||.|.|.|||||..
Zfish   135 PVMNKPYGNERTVYRAKFSSITDTDICNAMNRLGEPNKNEALSVDARQELDLRIGCAFTRFQTKY 199

  Fly   218 LQRLFPEKIADKLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKI--KVLHTIDD-LTVEFNWAR 279
            .|..: ..:...|||:|.||.|||||..||:.:|::|..|.:|.:  ||....|. ||::  |.|
Zfish   200 FQGKY-GNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPEAYWVVQAKVFKGKDSPLTLD--WDR 261

  Fly   280 NRLFDKEACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKETMTI 344
            .|:||::.  ..:.:.:|:....|.|:|||.|.|.|.||..|:||||.::.|..|.:..:.||.|
Zfish   262 VRVFDRDI--GQMFVNMAKTAKEARVDSVTKKEKAKQRPLALNTVEMLRVASSALGMGPQHTMQI 324

  Fly   345 AEKLYTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIEWGPN-PRNGNKSDQAHP 408
            ||:|||:|:||||||||..:.:.|.|...::.|.....||...:.::..|.| ||.|..:.. ||
Zfish   325 AERLYTQGYISYPRTETTHYPENFDLKGTLKQQANSTFWGETVKALLSEGINRPRKGADAGD-HP 388

  Fly   409 PIHPTKLA-ENLQGNEA-RVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANGLVIHERNYL 471
            ||.|.:.| ||..|::. |:|:.:.|||:|.||.|....:|.:...||.|.||.:|..:....|.
Zfish   389 PITPMRAASENELGSDGWRLYDYITRHFIATVSPDCKYLQTTISFSIATEGFTCSGKTLISAGYT 453

  Fly   472 DVYVYDKWSAKQ-IHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATHAEHI 535
            :|..:...:.:: :...|.|:.|...|:.|.|..|..|..||||:||.|||||||||||:...||
Zfish   454 EVMPWQGIAQEEALPVCERGEMFTVDEIKLLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHI 518

  Fly   536 NTIKERGYIGVLDKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKDPKVVLT 600
            |.|.:|.|:.|.....|.|..:|:.|..||..::..|..|.:|:..|..|.||..|:.:.:.||.
Zfish   519 NNICQRNYVTVESGRKLKPTNLGIVLVHGYYKIDADLVLPTIRSAVEKQLTLIALGKANFQQVLQ 583

  Fly   601 EQIAKYKQAYQQITDKITAMDAKISARINETPAANSAVQEGADG--------------------- 644
            ..:..:|:.:....|.|..||..:  .::.:|.|.:.......|
Zfish   584 HTLDIFKRKFHYFVDSIAGMDELM--EVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHC 646

  Fly   645 ----SAPSHGII------------------------QSIFQCPKCNEAPLALKPKKNQQGWYIGC 681
                |.|.:|.|                        :|...||.|...|.....||.     :||
Zfish   647 DETYSLPQNGAIKLYKELKCPLDEFELVLWTSGSRGKSYPVCPYCFSNPPFRDMKKG-----MGC 706

  Fly   682 N--NFPDCKNA-----------------VWLPTE-------CKDASVL-------------DECC 707
            |  ..|.|:::                 |:.||.       |...:|:             .:.|
Zfish   707 NECTHPSCQHSLNSLGIGQCVECETGVLVFDPTSGPKWRMACNKCNVVVHFFEQAHKVQVSQDSC 771

  Fly   708 PTCGDGYRMLKF-RLSTPYYRGVFGTPSG--WYKTCLPCDNLFRTTFNINLDSVK----KVGGIV 765
            .:|.....::.| :..:|       .|.|  .:..|:.||:.|:....:...:::    :.|
Zfish   772 DSCEASLVIVDFNKTRSP-------LPDGETQHTGCVFCDSAFQDLVELKHATMRHPMHRAG--- 826

  Fly   766 GEVRGGGGGPGPGPGGGGS 784
            |..||.|.|.|..|.|.|:
Zfish   827 GARRGRGKGRGRRPMGRGN 845

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TopA	27..618	CDD:223624	232/601 (39%)
		TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	63/161 (39%)
		TOP1Ac	194..608	CDD:238110	163/420 (39%)
		zf-C4_Topoisom	657..694	CDD:279704	12/55 (22%)
		YrdD	658..>744	CDD:223625	23/127 (18%)
		zf-GRF	1033..1076	CDD:284302
		zf-GRF	1150..1193	CDD:284302
top3b	XP_003198651.1	TOP1Bc	3..709	CDD:332419	252/716 (35%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG62408
OrthoDB	1	1.010	-	-		D104272at33208
OrthoFinder	1	1.000	-	-		FOG0000966
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.930