DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Top3alpha and Top3beta

DIOPT Version :9

Sequence 1:	NP_523602.2	Gene:	Top3alpha / 35236	FlyBaseID:	FBgn0040268	Length:	1250	Species:	Drosophila melanogaster
Sequence 2:	NP_001284938.1	Gene:	Top3beta / 31565	FlyBaseID:	FBgn0026015	Length:	875	Species:	Drosophila melanogaster

Alignment Length:	932	Identity:	293/932 - (31%)
Similarity:	418/932 - (44%)	Gaps:	184/932 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    29 LNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQ-NAKMVMTSVSGHMMQLAFQV 92
            |.||||...|.::||:||||....:.|........::....|.: :....||||.||:|.|.|..
  Fly     5 LMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHVMSLDFNK 69

  Fly    93 SYKNWRTVDPRSLFDAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEIIDVCR 157
            .|..|..|||..||....:|...:..:.:::.|..|.|||..|::|.|||:|||||.:|::|..:
  Fly    70 KYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEGENICFEVMDAVK 134

  Fly   158 AIKPNI----SVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMRL 218
            .:..|:    ..|||.||.||...:::|::.||.|::.::.:||.|.|||||.|.|.|||||
  Fly   135 HVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQELDLRIGCAFTRFQT--- 196

  Fly   219 QRLFPEKIAD---KLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKVLHTIDDLTVEFNWARN 280
             :.|.::..|   .|||||.||.|||||..:|:.:|:.|..|.||.:::|....::|:|  |||.
  Fly   197 -KFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHLQLLAGQPEVTLE--WARG 258

  Fly   281 RLFDKEACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKETMTIA 345
            |:|.|:..  .:||...:...:|.||||..|..:|.:|..|:|||:.::.|..|.:...:.|.||
  Fly   259 RVFKKDIA--IMLLNRVKEHKKATVESVASKEAYKSKPQALNTVELMRICSSGLGIGPFQAMQIA 321

  Fly   346 EKLYTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIEWGPNPRNGNKSDQAHPPI 410
            |:|||:|:||||||||||:...|.|..::.:.....|:|..|:.::.....||.| |....||||
  Fly   322 ERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFGEEARSILGDIQTPRKG-KDAGDHPPI 385

  Fly   411 HPTKLAENLQGNEA-------RVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANGLVIHER 468
            .|.||     ||.:       ||||.:.|||:..||:|.....|...:.:..|.|:....|:.:.
  Fly   386 TPMKL-----GNRSDFDRDTWRVYEFICRHFMGTVSRDLKYRVTTAKLSVGMETFSCTASVLIDA 445

  Fly   469 NYLDVYVYDKWSA----KQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDA 529
            .:..|..   |||    :....:..|.:....:|.|.|..|..|..|||::||.|||:|||||||
  Fly   446 GFTKVMT---WSAFGKDEPQPPFVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDA 507

  Fly   530 THAEHINTIKERGYIGVLDKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKD 594
            :...|||.|.:|.|:.:.:...|:|..:|:.|..||..::..|..|.:|.|.|..|.||.||..:
  Fly   508 SIPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLIAQGSAN 572

  Fly   595 PKVVLTEQIAKYKQAYQQITDKITAMDA--KIS----------------------------ARI- 628
            .:.||...|..:|..:......|.:|||  ::|                            ||:
  Fly   573 FQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLH 637

  Fly   629 ----NET---PAANSAVQ-------EGADGSAPSHGII-QSIFQCPKC-NEAPLALKPKKNQQGW 677
                :||   |..|..|.       :..|..|.|.|:. :|...||.| |..|.:..|...
  Fly   638 CSHCDETYALPIGNVKVYREFKCPLDDFDLLAFSTGVKGRSYPFCPYCYNHPPFSDMPHLG---- 698

  Fly   678 YIGCN-----NFPDCKNAVWLPTECKDASVLDECCPTCGDGYRMLKFRLSTPYYRGVFGTPSGWY 737
              |||     |.|...|.:.:.:           |..|..|..:|...|:          |: |.
  Fly   699 --GCNTCTNANCPHSLNTLGISS-----------CVECPTGVLVLDCTLA----------PT-WK 739

  Fly   738 KTCLPCD---NLFRTTFNINLDSVK--------------------------KVGGIV-------- 765
            ..|..||   |.|:....|.::..|                          |.|.|.
  Fly   740 LGCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHL 804

  Fly   766 ---------GEVRGGGGGPGPGPGGGGSGRGAGSGGWSSGPGGGGSGGGGGSGGWGSGTGGGGSG 821
                     ..||.|||.     .||.:||                 ||||.||...|:||..:.
  Fly   805 VEKHRAVASRPVRSGGGF-----RGGKAGR-----------------GGGGMGGAAFGSGGAVTA 847

  Fly   822 GWGSGTGGGGLGGGKGKKPGGE 843
            |.|...|||..|....|...|:
  Fly   848 GGGPNAGGGVRGSRVAKDKMGQ 869

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TopA	27..618	CDD:223624	221/607 (36%)
		TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	57/160 (36%)
		TOP1Ac	194..608	CDD:238110	160/427 (37%)
		zf-C4_Topoisom	657..694	CDD:279704	12/42 (29%)
		YrdD	658..>744	CDD:223625	20/91 (22%)
		zf-GRF	1033..1076	CDD:284302
		zf-GRF	1150..1193	CDD:284302
Top3beta	NP_001284938.1	PRK07219	4..781	CDD:235971	264/820 (32%)
		TOPRIM_TopoIA_TopoIII	4..166	CDD:173782	57/160 (36%)
		TOP1Ac	171..588	CDD:238110	161/433 (37%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45457281
Domainoid	1	1.000	104	1.000	Domainoid score	I334
eggNOG	1	0.900	-	-		E1_COG0550
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	118	1.000	Inparanoid score	I267
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D104272at33208
OrthoFinder	1	1.000	-	-		FOG0000966
OrthoInspector	1	1.000	-	-		otm51404
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	P	PTHR11390
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	9	8.900