DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top3alpha and Top3a

DIOPT Version :9

Sequence 1:	NP_523602.2	Gene:	Top3alpha / 35236	FlyBaseID:	FBgn0040268	Length:	1250	Species:	Drosophila melanogaster
Sequence 2:	XP_038943191.1	Gene:	Top3a / 303194	RGDID:	1309320	Length:	1003	Species:	Rattus norvegicus

Alignment Length:	1240	Identity:	511/1240 - (41%)
Similarity:	646/1240 - (52%)	Gaps:	295/1240 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 KYLNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQNAKMVMTSVSGHMMQLAFQ 91
            |.|.|||||||||.||.|||||..:|:||.|.:||:::|:..:.|||..|:|||||||::...||
  Rat    35 KVLCVAEKNDAAKGIADLLSNGRMRRKEGLSKFNKIYEFDYHLYGQNVTMIMTSVSGHLLAHDFQ 99

  Fly    92 VSYKNWRTVDPRSLFDAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEIIDVC 156
            :.::.|::.:|..||:|.::|....::..||:|||||.:.||.|:|||||||||||||:|||.||
  Rat   100 MQFRKWQSCNPLVLFEAEIEKYCPENFIDIKKTLERETQHCQALVIWTDCDREGENIGFEIIHVC 164

  Fly   157 RAIKPNISVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMRLQRL 221
            :|:|||:.|.||.|||||..|||.|.:.|.:||:|.|||||||.|||||.|||.|||||:||||:
  Rat   165 KAVKPNLQVLRARFSEITHHAVRAACEHLTEPDQRVSDAVDVRQELDLRIGAAFTRFQTLRLQRI 229

  Fly   222 FPEKIADKLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKVLHTIDDLTVEFNWARNRLFDKE 286
            |||.:|::||||||||.||||||.||:|.|:|||.|.|.:|||.|...|.||||||.|.|||:..
  Rat   230 FPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEVFHRIKVTHDHKDGTVEFNWKRYRLFNHT 294

  Fly   287 ACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKETMTIAEKLYTK 351
            ||.....||:  .||.|.|..|..|||.||||..|||||:|||.||||:::|||||.|||||||:
  Rat   295 ACLVLYQLCM--EDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAEKLYTQ 357

  Fly   352 GFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIE-WGPNPRNGNKSDQAHPPIHPTKL 415
            |:|||||||||.|.|:..|..|||.||....||||||.::| .||.||||||||||||||||||.
  Rat   358 GYISYPRTETNIFPKDLNLVTLVEQQTQDPHWGAFAQNILERGGPTPRNGNKSDQAHPPIHPTKY 422

  Fly   416 AENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANGLVIHERNYLDVYVYDKWS 480
            ..:|||::.|:||.:|||||||.|:||.|.||.|.||||.|:|.|:||:|..|||||||.||.||
  Rat   423 TSSLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLIILARNYLDVYPYDHWS 487

  Fly   481 AKQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATHAEHINTIKERGYIG 545
            .|.:..||.|.||:|:.|.:.:|.|:.|.||||||||||||||||||||||||||.|||.|.|:|
  Rat   488 DKLLPVYEQGFRFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVG 552

  Fly   546 VL-DKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKDPKVVLTEQIAKYKQA 609
            :. ||.|| ||.:||||.||||:|...::||.||||.|.||||||:|:||...||.:|:.||||.
  Rat   553 LTSDKRFL-PGHLGMGLVEGYDSMGYEMSKPNLRAELEADLKLICEGKKDKFQVLRQQVQKYKQV 616

  Fly   610 YQQITDKITAMDAKISARINETPAANSAVQEGADGSAPSHGIIQSIFQCPKCNEAPLALKPKKNQ 674
            :.:...|...:|..:|..:.|  ....|.||....:.|     :.:.:||:||: .:.||.||:
  Rat   617 FIEAVAKAKKLDEALSQYLGE--RTEVAQQEEIYPAMP-----EPVRKCPQCNK-DMVLKTKKS- 672

  Fly   675 QGWYIGCNNFPDCKNAVWLPTECKDASVLDECCPTCGDG--YRM-LKF-RLSTP-----YYRGVF 730
            .|:|:.|..||:|::|||.|....:||.....|..|...  ||: ||| |.|.|     .:.|..
  Rat   673 GGFYLSCTGFPECRSAVWFPDSVLEASRDSSVCSVCQPAPVYRLKLKFKRGSLPPSMPLEFVGCI 737

  Fly   731 GTPSGWYKTCLPCDNLFRTTFNINLDSVKKVGGIVGEVRGGGGGPGPGPGGGGSGRGAGSGGWSS 795
            |          .||...:..|.:..
  Rat   738 G----------GCDETLKEIFGLRF---------------------------------------- 752

  Fly   796 GPGGGGSGGGGGSGGWGSGTGGGGSGGWGSGTGGGGLGGGKGKKPGGESKKSATKKPPNEPKPKK 860
                                                                 ::.||...:|
  Rat   753 -----------------------------------------------------SRAPPRASQP-- 762

  Fly   861 TKEPKAAPNKKTSSKSSGSIRSFFTSAAPTNSASNGLDEFFDSNDGFEDAMLAAAESVESSSQPK 925
                            ||.:::        :.|.|.:|                 .|..|.|||
  Rat   763 ----------------SGHLQA--------SQALNRMD-----------------SSQRSLSQP- 785

  Fly   926 TISMVPLDDDIAAAFAADDDAEFEALVNGGTMPTESNGDQQLDKSLSEWIKEQDKADERPMLWGT 990
                                     |||..|.|::
  Rat   786 -------------------------LVNRHTGPSK------------------------------ 795

  Fly   991 RERASLGTAAPTPPPKPAAKRPRWDSVERDSTPPSSVPESETVLCTGCQQPARQNTVRKNGPNLG 1055
                                     :|.:...||::..|..:|.| .|.:.|...||||.|||.|
  Rat   796 -------------------------AVAQTLLPPTAAAEGNSVTC-NCGREAVLLTVRKQGPNQG 834

  Fly  1056 RLYYKCPKPDECNFFQWADEPPSSAKSKNSTGSAPQSTTSWGSNRVVTLPSIQQSNSQRGQSSMR 1120
            |.:|||...| ||||.|||       |.:.||..|.::.|......|..||        |..|..
  Rat   835 RHFYKCSGGD-CNFFLWAD-------SSHPTGGGPPTSVSRPPGNSVGCPS--------GFGSHM 883

  Fly  1121 SNSSSTVTITQTKTKQQERNTATPGDGE-EVMCNCGQLASQLTVRKDGPNQGRPFYAC-PTREKS 1183
            ....|                 ..|||: ...|.|||.|...||:|||||:||.|:.| ..||:.
  Rat   884 DGFGS-----------------LGGDGDGGASCLCGQPAVTRTVQKDGPNKGRQFHTCAKPREQQ 931

  Fly  1184 CGFFKWGDEDQNQGA-SSTSW----GSANRNPPGRSQPTAITSDG----PKTRRCGLCRKEGHTR 1239
            ||||:|.||:...|: ::.:|    |.|.|......:..|.:.|.    .|.|:|.||.:.||||
  Rat   932 CGFFQWVDENMAPGSFAAPAWPGGRGKAQRPEAASKRQRAGSLDAGSTVKKPRKCSLCHQPGHTR 996

  Fly  1240 NKCPR 1244
            ..||:
  Rat   997 PFCPQ 1001

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TopA	27..618	CDD:223624	366/592 (62%)
		TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	88/157 (56%)
		TOP1Ac	194..608	CDD:238110	270/415 (65%)
		zf-C4_Topoisom	657..694	CDD:279704	17/36 (47%)
		YrdD	658..>744	CDD:223625	32/94 (34%)
		zf-GRF	1033..1076	CDD:284302	24/42 (57%)
		zf-GRF	1150..1193	CDD:284302	24/43 (56%)
Top3a	XP_038943191.1	TOPRIM_TopoIA_TopoIII	35..193	CDD:173782	88/157 (56%)
		TOP1Ac	202..617	CDD:238110	272/417 (65%)
		zf-C4_Topoisom	655..694	CDD:307520	18/40 (45%)
		zf-GRF	813..854	CDD:399670	24/49 (49%)
		zf-GRF	898..941	CDD:399670	24/42 (57%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166339543
Domainoid	1	1.000	451	1.000	Domainoid score	I489
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H3394
Inparanoid	1	1.050	807	1.000	Inparanoid score	I462
OMA	1	1.010	-	-		QHG62408
OrthoDB	1	1.010	-	-		D104272at33208
OrthoFinder	1	1.000	-	-		FOG0000966
OrthoInspector	1	1.000	-	-		oto96858
orthoMCL	1	0.900	-	-		OOG6_101027
Panther	1	1.100	-	-	LDO	PTHR11390
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X3214
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	14	13.910