Protein Alignment Top3alpha and top3

DIOPT Version :9

• Sequence 1: NP_523602.2
• Gene: Top3alpha / 35236
• FlyBaseID: FBgn0040268
• Length: 1250
• Species: Drosophila melanogaster
• Sequence 2: NP_596761.1
• Gene: top3 / 2539881
• PomBaseID: SPBC16G5.12c
• Length: 622
• Species: Schizosaccharomyces pombe
• Alignment Length: 621
• Identity: 250/621 - (40%)
• Similarity: 377/621 - (60%)
• Gaps: 27/621 - (4%)

  Fly    26 MKYLNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQ--NAKMVMTSVSGHMMQL 88
            |:.|.|||||..||::|.:|..|..:||:..|.|.|.:||.....|.  ::.:.|||||||:.:.
pombe     1 MRVLCVAEKNSIAKSVASILGGGHVRRRDTRSKYVKNYDFSFNFGGNVGSSDVTMTSVSGHLTEA 65

  Fly    89 AFQVSYKNWRTVDPRSLFDAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEII 153
            :|...|.:|.:|....||||.:...|..:.|.:...:::|.|..|.|.||||||||||:||.||.
pombe    66 SFPSEYSSWSSVPQDVLFDAQIITSVSKNAEVLADNIKKEARNAQYLYIWTDCDREGEHIGVEIS 130

  Fly   154 DVCRAIKPNISVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMRL 218
            :|.||..|:|.|.||.|:.:....:..|.::.....|..:||||.|.|||.|.||..||.||::|
pombe   131 NVARASNPSIQVIRADFNNLERSHIISAAKRPRDVSKNAADAVDARIELDFRLGAIFTRLQTIQL 195

  Fly   219 QRLFPEKIADKLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKVLHTIDDLTVEFNWARNRLF 283
            |:.| :.:.:|:||||.||.||||||.:|::.:|.||.|.:|.::.:......|::|||.|.::|
pombe   196 QKSF-DILQNKIISYGPCQFPTLGFVVDRWQRVEDFVPETYWHLRFVDKRQGKTIQFNWERAKVF 259

  Fly   284 DKEACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKETMTIAEKL 348
            |:......|..||  ....|.|.::|.|||.|::|.||.|||:.|||.:.|::|||:|:.:||.|
pombe   260 DRLTTMIILENCL--ECKTAKVVNITQKPKTKYKPLPLSTVELTKLGPKHLRISAKKTLELAENL 322

  Fly   349 YTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVI--EWGPNPRNGNKSDQAHPPIH 411
            ||.||:|||||||:||.....|..:::..||.::|.::|:.::  ::.| ||.|..:|:||||||
pombe   323 YTNGFVSYPRTETDQFDSSMNLHAIIQKLTGAQEWDSYAEGLLAGDYRP-PRKGKHNDRAHPPIH 386

  Fly   412 PTKLAENL---QGNEARVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANGLVIHERNYLDV 473
            |.::....   ..:..:||||:.|.||||.|.:|.|:||||.:.:..|.|:..||::.|:|||:|
pombe   387 PVQMVHRSALPSQDHWKVYELITRRFLACCSDNAKGAETLVQVKMEEELFSKKGLLVTEKNYLEV 451

  Fly   474 YVYDKW-SAKQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATHAEHINT 537
            |.|:|| |:.|:..|...:.|:|..:.:.:.:|::|..:||.:|||||:.:|||||||.||||..
pombe   452 YPYEKWESSDQLPEYRLHEEFQPHILDMMDSSTSSPSYITEPELIALMDANGIGTDATMAEHIEK 516

  Fly   538 IKERGYI--------GVLDKGFLVPGVIGMGLYEGYD--AMELALAKPQLRAEFELDLKLICQGQ 592
            ::||.|:        ||.:   .||..:|:.|.:|||  .:|.:|.||.||.|.|:.||.|..||
pombe   517 VQEREYVIKRKKRGQGVTE---FVPSSLGVALAKGYDEIGLEWSLTKPFLRKEMEVQLKNIENGQ 578

  Fly   593 KDPKVVLTEQIAKYKQAYQQITDKITAMDAKISARI 628
            .:..|::...:.:::..:.....:...:  |.|.|:
pombe   579 LNRNVLVHMILTQFRDVFHLTKQRFDCL--KNSCRV 612

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TopA	27..618	CDD:223624	246/608 (40%)
		TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	64/159 (40%)
		TOP1Ac	194..608	CDD:238110	181/429 (42%)
		zf-C4_Topoisom	657..694	CDD:279704
		YrdD	658..>744	CDD:223625
		zf-GRF	1033..1076	CDD:284302
		zf-GRF	1150..1193	CDD:284302
top3	NP_596761.1	TOPRIM_TopoIA_TopoIII	2..162	CDD:173782	64/159 (40%)
		TopA	3..615	CDD:223624	249/619 (40%)
		TOP1Ac	168..596	CDD:238110	181/434 (42%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	337	1.000	Domainoid score	I195
eggNOG	1	0.900	-	-		E1_COG0550
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	325	1.000	Inparanoid score	I539
OMA	1	1.010	-	-		QHG62408
OrthoFinder	1	1.000	-	-		FOG0000966
OrthoInspector	1	1.000	-	-		oto101144
orthoMCL	1	0.900	-	-		OOG6_101027
Panther	1	1.100	-	-	LDO	PTHR11390
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1537
SonicParanoid	1	1.000	-	-		X3214
TreeFam	1	0.960	-	-
	13	12.860