DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top3alpha and Top3b

DIOPT Version :9

Sequence 1:	NP_523602.2	Gene:	Top3alpha / 35236	FlyBaseID:	FBgn0040268	Length:	1250	Species:	Drosophila melanogaster
Sequence 2:	NP_001313505.1	Gene:	Top3b / 21976	MGIID:	1333803	Length:	862	Species:	Mus musculus

Alignment Length:	944	Identity:	278/944 - (29%)
Similarity:	405/944 - (42%)	Gaps:	197/944 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    29 LNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQNAKMVMTSVSGHMMQLAFQVS 93
            |.||||...|::||.:||.|.....:|.:....|..:.....||.....||||.||:|.|.|...
Mouse     5 LMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVMTLDFLGK 69

  Fly    94 YKNWRTVDPRSLF-DAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEIIDVCR 157
            |..|..|||..|| .||.:|...:....:.:.|:.|.|||..:::|.|||:|||||.:|::|...
Mouse    70 YNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEGENICFEVLDAVL 134

  Fly   158 AIKPNI-----SVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMR 217
            .:..|.     :|:||.||.||...:..|:.:|.:||..::.:||.|.|||||.|.|.|||||
Mouse   135 PVMNNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQELDLRIGCAFTRFQT-- 197

  Fly   218 LQRLFPEKIAD---KLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKV-LHTIDDLTVEFNWA 278
              :.|..|..|   .|||:|.||.|||||..||:.:|::|..|.:|.::. :||..:.::..:|.
Mouse   198 --KYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVHTDKEESLLLDWD 260

  Fly   279 RNRLFDKEACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKETMT 343
            |.|:||.|..:.:|.:...|.:  |.||:.:.|.|.|.||..|:||||.::.|..|.:..:..|.
Mouse   261 RVRVFDWEIAQMFLNMTKLEKE--AWVEATSRKEKAKQRPLALNTVEMLRVASSALGMGPQHAMQ 323

  Fly   344 IAEKLYTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIEWGPN-PRNGNKSDQAH 407
            |||:|||:|:||||||||..:.:.|.|...:..|..|..|....::::..|.| ||.|:.:.. |
Mouse   324 IAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADSVKQLLAEGINRPRKGHDAGD-H 387

  Fly   408 PPIHPTKLA--ENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIAGEKFTANGLVIHERNY 470
            |||.|.|.|  ..|.|:..|:||.:.|||:|.||.|....::.:...|..|.||..|..:....:
Mouse   388 PPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPEHFTCMGKTVISPGF 452

  Fly   471 LDVYVYDKWSA----KQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATH 531
            .::.   .|.:    :.:...:.|..|...||.:.|..|:.|..||||:||.|||||||||||:.
Mouse   453 TEIM---PWQSVPLEESLPTCQKGDTFTVGEVKMLEKQTSPPDYLTEAELITLMEKHGIGTDASI 514

  Fly   532 AEHINTIKERGYIGVLDKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKDPK 596
            ..|||.|.:|.|:.|.....|.|..:|:.|..||..::..|..|.:|:..|..|.||.||:.|..
Mouse   515 PVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYH 579

  Fly   597 VVLTEQIAKYKQAYQQITDKITAMDAKISARINETPAANSAVQEGADG----------------- 644
            .||...:..:|:.:....|.|..||..:  .::.:|.|.:.......|
Mouse   580 QVLGHTLDIFKRKFHYFVDSIAGMDELM--EVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLH 642

  Fly   645 --------SAPSHGII------------------------QSIFQCPKCNEAPLALKPKKNQQGW 677
                    :.|.:|.|                        :|...||.|...|.....||.
Mouse   643 CSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKG---- 703

  Fly   678 YIGCNNFP-----------------DCKNAVWL--PTE-------CKDASVLDEC---------- 706
             :|||...                 :|:|.|.:  ||.       |...:|:..|
Mouse   704 -MGCNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHRVRVS 767

  Fly   707 ---CPTCGDGYRMLKFRLSTPYYRGVFGTPSGWYKTCLPCDNLFRTTFNINLDSVKKVGGIVGEV 768
               |.||......:.|..:.....|.....:|    |:.||.:|:     .|..:|.........
Mouse   768 ADTCNTCEAALLDVDFNKAKSPLPGNETQHTG----CIFCDPVFQ-----ELVELKHAASCHPMH 823

  Fly   769 RGGGGGPGPGPGGGGSGRGAGSGGWSSGPGGGGSGGGGGSGGWGSGTGGGGSGGWGSGTGGGGLG 833
            |||        .|...|||.|.|
Mouse   824 RGG--------PGRRQGRGRGRG------------------------------------------ 838

  Fly   834 GGKGKKPGGESKKSATKKPPNEPKPKKTKEPKAA 867
                            ::||.:|.|::.|:..:|
Mouse   839 ----------------RRPPGKPNPRRPKDKMSA 856

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TopA	27..618	CDD:223624	224/605 (37%)
		TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	59/161 (37%)
		TOP1Ac	194..608	CDD:238110	160/424 (38%)
		zf-C4_Topoisom	657..694	CDD:279704	12/55 (22%)
		YrdD	658..>744	CDD:223625	24/124 (19%)
		zf-GRF	1033..1076	CDD:284302
		zf-GRF	1150..1193	CDD:284302
Top3b	NP_001313505.1	TOPRIM_TopoIA_TopoIII	3..167	CDD:173782	59/161 (37%)
		Topoisom_bac	169..582	CDD:395899	160/422 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	820..855		14/100 (14%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0550
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG62408
OrthoDB	1	1.010	-	-		D104272at33208
OrthoFinder	1	1.000	-	-		FOG0000966
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.830