DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top3alpha and Top3a

DIOPT Version :9

Sequence 1:	NP_523602.2	Gene:	Top3alpha / 35236	FlyBaseID:	FBgn0040268	Length:	1250	Species:	Drosophila melanogaster
Sequence 2:	NP_033436.1	Gene:	Top3a / 21975	MGIID:	1197527	Length:	1003	Species:	Mus musculus

Alignment Length:	1265	Identity:	522/1265 - (41%)
Similarity:	664/1265 - (52%)	Gaps:	311/1265 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly    15 HRRYCANPG------QAM--------KYLNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDF 65
            |||    ||      .||        |.|.|||||||||.||.|||||..:|:||.|.:||:::|
Mouse    13 HRR----PGGRALSRAAMEVAFRGVRKVLCVAEKNDAAKGIADLLSNGRMRRKEGLSKFNKIYEF 73

  Fly    66 EAPVRGQNAKMVMTSVSGHMMQLAFQVSYKNWRTVDPRSLFDAPVKKGVGSDYEPIKRTLEREVR 130
            :..:.|||..|:|||||||::...||:.::.|::.:|..||:|.::|....::..||:|||||..
Mouse    74 DYHLYGQNVTMIMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFIDIKKTLERETH 138

  Fly   131 GCQGLIIWTDCDREGENIGYEIIDVCRAIKPNISVYRATFSEITTVAVRRALQQLGQPDKRQSDA 195
            .||.|:|||||||||||||:|||.||:|:|||:.|.||.|||||..|||.|.:.|.:||:|.|||
Mouse   139 HCQALVIWTDCDREGENIGFEIIHVCKAVKPNLRVLRARFSEITPHAVRTACENLTEPDQRVSDA 203

  Fly   196 VDVRTELDLRTGAAITRFQTMRLQRLFPEKIADKLISYGSCQIPTLGFVAERYKEIEAFVSEPFW 260
            ||||.|||||.|||.|||||:||||:|||.:|::||||||||.||||||.||:|.|:|||.|.|.
Mouse   204 VDVRQELDLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEVFH 268

  Fly   261 KIKVLHTIDDLTVEFNWARNRLFDKEACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVE 325
            ||||.|...|.||||||.|.|||:..||.....||:  .||.|.|..|..|||.||||..|||||
Mouse   269 KIKVTHDHKDGTVEFNWKRYRLFNHTACLVLYQLCM--EDPMATVVEVRSKPKSKWRPQALDTVE 331

  Fly   326 MEKLGSRKLKLSAKETMTIAEKLYTKGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRV 390
            :|||.||||:::|||||.|||||||:|:|||||||||.|.|:..|..|||.||....||||||.:
Mouse   332 LEKLASRKLRINAKETMRIAEKLYTQGYISYPRTETNIFPKDLNLVALVEQQTVDPHWGAFAQTI 396

  Fly   391 IE-WGPNPRNGNKSDQAHPPIHPTKLAENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIA 454
            :| .||.||||:|||||||||||||....|||::.|:||.:|||||||.|:||.|.||.|.||||
Mouse   397 LERGGPTPRNGSKSDQAHPPIHPTKYTSGLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIA 461

  Fly   455 GEKFTANGLVIHERNYLDVYVYDKWSAKQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIAL 519
            .|:|.|:||:|..|||||||.||.||.|.:..||.|..|:|:.|.:.:|.|:.|.||||||||||
Mouse   462 QERFVAHGLIILARNYLDVYPYDHWSDKLLPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIAL 526

  Fly   520 MEKHGIGTDATHAEHINTIKERGYIGVL-DKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFEL 583
            ||||||||||||||||.|||.|.|:|:. ||.|| ||.:||||.||||:|...::||.||||.|.
Mouse   527 MEKHGIGTDATHAEHIETIKARMYVGLTSDKRFL-PGHLGMGLVEGYDSMGYEMSKPDLRAELEA 590

  Fly   584 DLKLICQGQKDPKVVLTEQIAKYKQAYQQITDKITAMDAKISARINETPAANSAVQEGADGSAPS 648
            ||||||:|:||...||.:|:.||||.:.:...|...:|..:|..:.|  ....|.||....:.|
Mouse   591 DLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEALSQYLGE--RTEMAQQEEIYPAMP- 652

  Fly   649 HGIIQSIFQCPKCNEAPLALKPKKNQQGWYIGCNNFPDCKNAVWLPTECKDASVLDECCPTCGDG 713
                :.:.:||:||: .:.||.||: .|:|:.|..||:|::|||.|....:||..:..|..|...
Mouse   653 ----EPVRKCPQCNK-DMVLKTKKS-GGFYLSCMGFPECRSAVWFPDSVLEASRDNSVCSVCQPP 711

  Fly   714 --YRM-LKF-RLSTP-----YYRGVFGTPSGWYKTCLPCDNLFRTTFNINLDSVKKVGGIVGEVR 769
              ||: ||| |.|.|     .:.|..|          .||           :::|::.|:
Mouse   712 PVYRLKLKFKRGSLPPAMPLEFVGCIG----------GCD-----------ETLKEIFGL----- 750

  Fly   770 GGGGGPGPGPGGGGSGRGAGSGGWSSGPGGGGSGGGGGSGGWGSGTGGGGSGGWGSGTGGGGLGG 834

Mouse   751 ----------------------------------------------------------------- 750

  Fly   835 GKGKKPGGESKKSATKKPPNEPKPKKTKEPKAAPNKKTSSKSSGSIRSFFTSAAPTNSASNGLDE 899
                                       :.|:|.|.   :|:.||.:::        :.|.|.:|
Mouse   751 ---------------------------RFPRALPR---ASQPSGHLQA--------SQALNRMD- 776

  Fly   900 FFDSNDGFEDAMLAAAESVESSSQPKTISMVPLDDDIAAAFAADDDAEFEALVNGGTMPTESNGD 964
                            .|..:.|||                          |||..|.|::
Mouse   777 ----------------SSQHNLSQP--------------------------LVNRHTRPSK---- 795

  Fly   965 QQLDKSLSEWIKEQDKADERPMLWGTRERASLGTAAPTPPPKPAAKRPRWDSVERDSTPPSSVPE 1029
                                                               :|.:...||::..|
Mouse   796 ---------------------------------------------------TVAQALLPPTTAGE 809

  Fly  1030 SETVLCTGCQQPARQNTVRKNGPNLGRLYYKCPKPDECNFFQWADEPPSSAKSKNSTGSAPQSTT 1094
            |.:|.| .|.:.|...||||.|||.||.:|||...| ||||.|||       |.:|||....::.
Mouse   810 SNSVTC-NCGREAVLLTVRKQGPNQGRHFYKCSNGD-CNFFLWAD-------SSHSTGGGTPTSA 865

  Fly  1095 SWGSNRVVTLPSIQQSNSQRGQSSMRSNSSSTVTITQTKTKQQERNTATPGDGEEVMCNCGQLAS 1159
            |......|..||...|:.. |..|:.|:|..                .||       |.|||.|.
Mouse   866 SGPPGSSVGCPSSVGSHMD-GFGSLGSDSDG----------------GTP-------CLCGQPAV 906

  Fly  1160 QLTVRKDGPNQGRPFYAC-PTREKSCGFFKWGDEDQNQGA-SSTSW----GSANRNPPGRSQPTA 1218
            ..||:|||||:||.|:.| ..||:.||||:|.||:...|: ::.:|    |.|.|......:|.|
Mouse   907 TRTVQKDGPNKGRQFHTCAKPREQQCGFFQWVDENVAPGSFAAPAWPGGRGKAQRPEAASKRPRA 971

  Fly  1219 ITSDG----PKTRRCGLCRKEGHTRNKCPR 1244
            .:||.    .|.|:|.||.:.||||..||:
Mouse   972 GSSDAGSTVKKPRKCSLCHQPGHTRTFCPQ 1001

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TopA	27..618	CDD:223624	365/592 (62%)
		TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	88/157 (56%)
		TOP1Ac	194..608	CDD:238110	269/415 (65%)
		zf-C4_Topoisom	657..694	CDD:279704	17/36 (47%)
		YrdD	658..>744	CDD:223625	32/94 (34%)
		zf-GRF	1033..1076	CDD:284302	24/42 (57%)
		zf-GRF	1150..1193	CDD:284302	24/43 (56%)
Top3a	NP_033436.1	TopA	35..635	CDD:223624	367/602 (61%)
		TOPRIM_TopoIA_TopoIII	35..193	CDD:173782	88/157 (56%)
		TOP1Ac	202..617	CDD:238110	271/417 (65%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	400..426		19/25 (76%)
		YrdD	<652..722	CDD:223625	28/76 (37%)
		zf-C4_Topoisom	655..694	CDD:279704	18/40 (45%)
		zf-GRF	813..854	CDD:284302	24/49 (49%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	856..888		9/32 (28%)
		zf-GRF	897..941	CDD:284302	26/50 (52%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	946..991		13/44 (30%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167835906
Domainoid	1	1.000	521	1.000	Domainoid score	I303
eggNOG	1	0.900	-	-		E1_COG0550
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H3394
Inparanoid	1	1.050	886	1.000	Inparanoid score	I397
Isobase	1	0.950	-	0.919607	Normalized mean entropy	S1204
OMA	1	1.010	-	-		QHG62408
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000966
OrthoInspector	1	1.000	-	-		oto93316
orthoMCL	1	0.900	-	-		OOG6_101027
Panther	1	1.100	-	-	LDO	PTHR11390
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1537
SonicParanoid	1	1.000	-	-		X3214
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	16	15.740