Protein Alignment Top3alpha and Top3alpha

DIOPT Version :10

• Sequence 1: NP_523602.2
• Gene: Top3alpha / 35236
• FlyBaseID: FBgn0040268
• Length: 1250
• Species: Drosophila melanogaster
• Sequence 2: XP_061511099.1
• Gene: Top3alpha / 1279455
• VectorBaseID: AGAMI1_006611
• Length: 1008
• Species: Anopheles gambiae
• Alignment Length: 1244
• Identity: 556/1244 - (44%)
• Similarity: 704/1244 - (56%)
• Gaps: 290/1244 - (23%)

  Fly    26 MKYLNVAEKNDAAKTIAGLLSNGAAQRREGYSVYNKVFDFEAPVRGQNAKMVMTSVSGHMMQLAF 90
            |||||||||||||||||||||.|::|||||||.|||:::|...:.||:.:|:|||||||::...|
Mosquito    29 MKYLNVAEKNDAAKTIAGLLSRGSSQRREGYSPYNKIYEFSYNLHGQSIQMIMTSVSGHLLTHEF 93

  Fly    91 QVSYKNWRTVDPRSLFDAPVKKGVGSDYEPIKRTLEREVRGCQGLIIWTDCDREGENIGYEIIDV 155
            ..|::.|.:..|..||||||:|.....||.||:|||||||||..|||||||||||||||||||:|
Mosquito    94 LPSFRGWHSCSPEDLFDAPVRKNCPEQYEKIKKTLEREVRGCSALIIWTDCDREGENIGYEIIEV 158

  Fly   156 CRAIKPNISVYRATFSEITTVAVRRALQQLGQPDKRQSDAVDVRTELDLRTGAAITRFQTMRLQR 220
            |||:||.:.|:||.|||||..:::||::.|.|||.||:|||:||:|||||.|||.|||||:|||:
Mosquito   159 CRAVKPQLRVFRAKFSEITAPSIKRAIENLVQPDARQNDAVNVRSELDLRIGAAFTRFQTLRLQK 223

  Fly   221 LFPEKIADKLISYGSCQIPTLGFVAERYKEIEAFVSEPFWKIKVLHTIDDLTVEFNWARNRLFDK 285
            .||:.|::.|:||||||||||||||:||||||.|:.:.|||||:.||||:|||||:|:|||||||
Mosquito   224 SFPQDISNNLVSYGSCQIPTLGFVAQRYKEIENFIPQTFWKIKLTHTIDELTVEFHWSRNRLFDK 288

  Fly   286 EACENYLLLCLAEPDPRALVESVTVKPKHKWRPTPLDTVEMEKLGSRKLKLSAKETMTIAEKLYT 350
            :.||.||:||  :..|.|.|.:||.|||:||||||:||||:|||||||||::||:.|||||||||
Mosquito   289 QCCEAYLMLC--QTTPTAKVVNVTQKPKNKWRPTPMDTVELEKLGSRKLKMNAKQVMTIAEKLYT 351

  Fly   351 KGFISYPRTETNQFSKEFALAPLVEMQTGHRDWGAFAQRVIEWGPNPRNGNKSDQAHPPIHPTKL 415
            :|.|||||||||.|:.:..|||||:.|.....||:||::|::||.|||||.||||||||||||||
Mosquito   352 QGIISYPRTETNMFTGDMKLAPLVQAQVASDQWGSFAEKVLQWGVNPRNGKKSDQAHPPIHPTKL 416

  Fly   416 AENLQGNEARVYELVVRHFLACVSKDAVGSETLVHIDIA-GEKFTANGLVIHERNYLDVYVYDKW 479
            ..||.|:|.|||||:.||||||||:||.||||:|::.:| .|:|||:||.|||||||:||:||:|
Mosquito   417 PTNLFGDEWRVYELIARHFLACVSRDATGSETIVNVVVAEEEEFTASGLCIHERNYLEVYLYDRW 481

  Fly   480 SAKQIHHYENGQRFEPTEVSLHEGATTAPPLLTEADLIALMEKHGIGTDATHAEHINTIKERGYI 544
            :||:||.|:.|..|||||:.||||:||||.:||||||||||||||||||||||||||||||||||
Mosquito   482 NAKEIHSYQVGHTFEPTELGLHEGSTTAPNMLTEADLIALMEKHGIGTDATHAEHINTIKERGYI 546

  Fly   545 GVLDKGFLVPGVIGMGLYEGYDAMELALAKPQLRAEFELDLKLICQGQKDPKVVLTEQIAKYKQA 609
            |..|:||||||.:||||.|||:.|||.||.|:|||..|.||||:|:|:|:|..||.|||||||:.
Mosquito   547 GERDRGFLVPGTLGMGLVEGYEMMELRLAHPELRAGLEADLKLVCEGRKNPNDVLAEQIAKYKEV 611

  Fly   610 YQQITDKITAMDAKISARINETP------AANSAVQEGADGSAPSHGIIQSIFQCPKCNEAPLAL 668
            |:.::.|..|:|..:..|:|:||      |..||..  |.||||    ::.:.:||||.. .:.|
Mosquito   612 YRIMSQKARALDRAMGQRLNQTPQEPPPDAGASAAT--ATGSAP----MREVCKCPKCGH-KMCL 669

  Fly   669 KPKKNQQGWYIGCNNFPDCKNAVWLPTECKDASVLDECCPTCGDGYRMLKFRLSTPYYRGVFGTP 733
            :.|::..|:|:||..||:|:|.:|.....::.:|||:.|..||.....:||| |..:|..:..|.
Mosquito   670 RTKRDSSGYYLGCVAFPECRNNIWFDDSMREINVLDDTCARCGSKKMTVKFR-SVRFYALLQSTD 733

  Fly   734 SGWYKTCLPCDNLFRTTFNINLDSVKKVGGIVGEVRGGGGGPGPGPGGGGSGRGAGSGGWSSGPG 798
            ...|:.|:.||:.||..||||..||:...|                       ...|...::.|
Mosquito   734 MDEYQFCIVCDDKFRNLFNINESSVRVAAG-----------------------ARSSSNTTTVP- 774

  Fly   799 GGGSGGGGGSGGWGSGTGGGGSGGWGSGTGGGGLGGGKGKKPGGESKKSATKKPPNEPKPKKTKE 863
                         .:......:..||:|.                         .:|.:|..|..
Mosquito   775 -------------VAQPARSSTSTWGTGN-------------------------QSERRPANTAH 801

  Fly   864 PKAAPNKKTSSKSSGSIRSFFTSAAPTNSASNGLDEFFDSNDGFEDAMLAAAESVESSSQPKTIS 928
            ...:.....|:.|:||..|:.|.    .|.||.......||.|
Mosquito   802 NTTSSGIGRSNSSAGSSSSWGTG----QSTSNNWGGSQSSNRG---------------------- 840

  Fly   929 MVPLDDDIAAAFAADDDAEFEALVNGGTMPTESNGDQQLDKSLSEWIKEQDKADERPMLWGTRER 993
                                     ||:..|                           .||
Mosquito   841 -------------------------GGSSTT---------------------------TWG---- 849

  Fly   994 ASLGTAAPTPPPKPAAKRPRWDSVERDSTPPSSVPESETVLCTGCQQPARQNTVRKNGPNLGRLY 1058
                        |||..|
Mosquito   850 ------------KPANNR----------------------------------------------- 855

  Fly  1059 YKCPKPDECNFFQWADEPPSSAKSKNSTGSAPQSTTSWGSNRVVTLPSIQQSNSQRGQSSMRSNS 1123
                          ||.|..........|.|.|:  ||.:      ||
Mosquito   856 --------------ADGPAKKGGGFGDAGGAKQN--SWHN------PS----------------- 881

  Fly  1124 SSTVTITQTKTKQQERNTATPGDGE---EVMCNCGQLASQLTVRKDGPNQGRPFYACPTREKSCG 1185
                            |::..|.||   ||||.||..||:.||:|||||:||||::||.:..|||
Mosquito   882 ----------------NSSRVGIGEEDGEVMCRCGTPASRFTVKKDGPNKGRPFFSCPNQASSCG 930

  Fly  1186 FFKWGDEDQ--------NQGASSTSWGSA----NRNPPGRSQPTAITSDGPKTRRCGLCRKEGHT 1238
            |||||||:.        ..|..|.||||.    :.:..|||...........||:|||||:||||
Mosquito   931 FFKWGDENMPPASSHNGGTGGGSVSWGSGGVSNSGSSWGRSSNDTTNKTSRTTRKCGLCRQEGHT 995

  Fly  1239 RNKCPRKDE 1247
            :||||::.|
Mosquito   996 KNKCPQRGE 1004

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top3alpha	NP_523602.2	TOPRIM_TopoIA_TopoIII	27..185	CDD:173782	101/157 (64%)
		TOP1Ac	194..608	CDD:238110	292/414 (71%)
		zf-C4_Topoisom	658..694	CDD:307520	15/35 (43%)
		zf-GRF	1033..1076	CDD:462017	2/42 (5%)
		zf-GRF	1150..1193	CDD:462017	29/42 (69%)
Top3alpha	XP_061511099.1	None