Protein Alignment Top2 and gyrB

DIOPT Version :10

• Sequence 1: NP_476760.1
• Gene: Top2 / 35225
• FlyBaseID: FBgn0284220
• Length: 1447
• Species: Drosophila melanogaster
• Sequence 2: YP_026241.1
• Gene: gyrB / 948211
• ECOCYCID: EG10424
• Length: 804
• Species: Escherichia coli
• Alignment Length: 870
• Identity: 194/870 - (22%)
• Similarity: 344/870 - (39%)
• Gaps: 205/870 - (23%)

  Fly     1 MENGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKI 65
            |.|...:.||:.:    ..|:.:..||..|||..:         |.:             ||:.:
E. coli     1 MSNSYDSSSIKVL----KGLDAVRKRPGMYIGDTD---------DGT-------------GLHHM 39

  Fly    66 FDEILVNAADNK---QRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLL 127
            ..|::.||.|..   ...:.:.||..|     |.|||.::|:|||..:|.|:.:....:|...|.
E. coli    40 VFEVVDNAIDEALAGHCKEIIVTIHAD-----NSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLH 99

  Fly   128 TSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNG 192
            ....::|:..||:||.:|.|..:.|..|..  :|...:...|..:|.:.:.:.:|......:...
E. coli   100 AGGKFDDNSYKVSGGLHGVGVSVVNALSQK--LELVIQREGKIHRQIYEHGVPQAPLAVTGETEK 162

  Fly   193 TDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNK----------LGVRN 247
            |. |.:.|.|.|..|  ..:.|....::::|..:::..:.|||:.|...:          .|::.
E. coli   163 TG-TMVRFWPSLETF--TNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKA 224

  Fly   248 FKDYID-----LHIK----NTDDDSGPPIKIVHEVANERWEVACCPSDRGFQQ--VSFVNSIATY 301
            |.:|::     :|..    :|:.|.     |..|||.: |       :.|||:  ..|.|:|...
E. coli   225 FVEYLNKNKTPIHPNIFYFSTEKDG-----IGVEVALQ-W-------NDGFQENIYCFTNNIPQR 276

  Fly   302 KGGRHVDHVVDNLIKQLLEVLKKK---NKGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENM- 362
            .||.|:......:.:.|...:.|:   .|..::......|..|...|:..:.:|.|.||||:.:
E. coli   277 DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLV 341

  Fly   363 ------TLQQKGFGSKCTLSEKFINNMSKSGIVESVL---AWAKFKAQNDIAKTGGRKSSKIKGI 418
                  .::|:   ....|:|..:.|.:.:.||...:   |.|:..|:.....|..:.:..:.|:
E. coli   342 SSEVKSAVEQQ---MNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGL 403

  Fly   419 P-KLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLS 482
            | ||.|..|   ::.....|.|.|||||...|..|.   .|....:.||:||:|||.:|.|.::.
E. coli   404 PGKLADCQE---RDPALSELYLVEGDSAGGSAKQGR---NRKNQAILPLKGKILNVEKARFDKML 462

  Fly   483 ENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRL 547
            .:.|:..|...:|....:.....|   .|||..::||||.|.|||||:.||:.|.:...||::..
E. coli   463 SSQEVATLITALGCGIGRDEYNPD---KLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVER 524

  Fly   548 PFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQD-- 610
            ..:.....|:.|..|..:|                                       :|.:|
E. coli   525 GHVYIAQPPLYKVKKGKQE---------------------------------------QYIKDDE 550

  Fly   611 -MDRHRILFKYDG----------SVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERY 664
             ||:::|....||          ::..|::....|:.:...:.:   |.|:   ||....:.:..
E. coli   551 AMDQYQISIALDGATLHTNASAPALAGEALEKLVSEYNATQKMI---NRME---RRYPKAMLKEL 609

  Fly   665 LYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSG 729
            :|   ..::|.||..:.:.|       .|.:.:||..|              |||.:      .|
E. coli   610 IY---QPTLTEADLSDEQTV-------TRWVNALVSEL--------------NDKEQ------HG 644

  Fly   730 SVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLT 794
            |..:...:.:.|.:|...||.:..:  |.:....|:  .:|   ::||:    .|.|.|:...|.
E. coli   645 SQWKFDVHTNAEQNLFEPIVRVRTH--GVDTDYPLD--HEF---ITGGE----YRRICTLGEKLR 698

  Fly   795 RLIYHPLDDPLLDYQVDDGQKIEPL 819
            .|:..       |..::.|::.:|:
E. coli   699 GLLEE-------DAFIERGERRQPV 716

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_476760.1	PTZ00108	5..1378	CDD:240271	192/866 (22%)
gyrB	YP_026241.1	gyrB	1..804	CDD:237860	194/870 (22%)