Protein Alignment Top2 and parE

DIOPT Version :10

• Sequence 1: NP_476760.1
• Gene: Top2 / 35225
• FlyBaseID: FBgn0284220
• Length: 1447
• Species: Drosophila melanogaster
• Sequence 2: NP_417502.1
• Gene: parE / 947501
• ECOCYCID: EG10687
• Length: 630
• Species: Escherichia coli
• Alignment Length: 697
• Identity: 162/697 - (23%)
• Similarity: 267/697 - (38%)
• Gaps: 154/697 - (22%)

  Fly    20 LEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMN 84
            ||.:..||..|..:.........|.|||.:..       :.|..|..|.||       ..|:|:.
E. coli    16 LEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEA-------LAGHAKRVDVIL-------HADQSLE 66

  Fly    85 TIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAK 149
            .|              ::|:|:||.:|.|:.:....:|...|.....:::...:.:||.:|.|..
E. coli    67 VI--------------DDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGIS 117

  Fly   150 LCNIFSTSFTVETATRE----YKKSFKQTWGNNMGKASDVQI-----KDFNGTDYTRITFSPDLA 205
            :.|..|.  .||...|.    |..:|:     |..|..|:|:     |...||.   :.|.||..
E. coli   118 VVNALSK--RVEVNVRRDGQVYNIAFE-----NGEKVQDLQVVGTCGKRNTGTS---VHFWPDET 172

  Fly   206 KFKMDRLDEDIVALMSRRAYDVAASS---KGVSVFLNGNKLGVRNFKDYIDLHIKNTDD----DS 263
            .|...|..      :||..:.:.|.:   .||.:          .|||    .|.||:.    ..
E. coli   173 FFDSPRFS------VSRLTHVLKAKAVLCPGVEI----------TFKD----EINNTEQRWCYQD 217

  Fly   264 G---------------PPIKIVHEVANER----WEVACCPSDRGFQQVSFVNSIATYKGGRHVDH 309
            |               |....:...|.:.    |.:...|........|:||.|.|.:||.||:.
E. coli   218 GLNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNG 282

  Fly   310 VVDNLIKQLLEVLKKKN--KGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSK 372
            :...|:..:.|..:.:|  ..|:.:....:.:.....::..:::|.|..||||.::.:|
E. coli   283 LRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQ------ 341

  Fly   373 CTLSEKFINNMSKSGIV-ESVLAW-------AKFKAQNDIAKTGGRKSSKIKGIPKLEDANEAGG 429
            |.   .|:     ||:| ::.:.|       |:..|:..|:....|..:..|.:.|...:..|..
E. coli   342 CA---AFV-----SGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALP 398

  Fly   430 KNSIKCT--------LILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAE 486
            .....||        |.|.|||||...|....   .|:...:.||:||:||..|.:..::..:.|
E. coli   399 GKLADCTAQDLNRTELFLVEGDSAGGSAKQAR---DREYQAIMPLKGKILNTWEVSSDEVLASQE 460

  Fly   487 INNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLE 551
            ::::...||:.     ...|||..|||||:.|:.|.|.||.||..||......::..|::...:.
E. coli   461 VHDISVAIGID-----PDSDDLSQLRYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVY 520

  Fly   552 EFITPIVKATKKNEELSFYSLPE------FEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQD 610
            ..:.|:.:.....|  .:|:|.|      .|:.|......   |::.:||||.....:.:|...|
E. coli   521 VALPPLYRIDLGKE--VYYALTEEEKEGVLEQLKRKKGKP---NVQRFKGLGEMNPMQLRETTLD 580

  Fly   611 MDRHRILFKYDGSVDDES-------IVMAFSKKHIESRKVWLTNHMD 650
            .:..|::   ..::|||.       :.|..:||..|.|:.||....|
E. coli   581 PNTRRLV---QLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD 624

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_476760.1	PTZ00108	5..1378	CDD:240271	162/697 (23%)
parE	NP_417502.1	parE_Gneg	2..626	CDD:130127	162/697 (23%)