DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top2 and TOP2

DIOPT Version :9

Sequence 1:	NP_001260580.1	Gene:	Top2 / 35225	FlyBaseID:	FBgn0284220	Length:	1447	Species:	Drosophila melanogaster
Sequence 2:	NP_014311.3	Gene:	TOP2 / 855636	SGDID:	S000005032	Length:	1428	Species:	Saccharomyces cerevisiae

Alignment Length:	1506	Identity:	648/1506 - (43%)
Similarity:	903/1506 - (59%)	Gaps:	153/1506 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     8 LSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVN 72
            :|....|||.|||||||.|||:||||||..::|.|:||...:.|::|.::.||||:|||||||||
Yeast     6 VSASDKYQKISQLEHILKRPDTYIGSVETQEQLQWIYDEETDCMIEKNVTIVPGLFKIFDEILVN 70

  Fly    73 AADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEK 137
            |||||.||.||..|.::|..|.:.:.|.|:|:|||:.:|.::.:|:|.||||||||||||:||||
Yeast    71 AADNKVRDPSMKRIDVNIHAEEHTIEVKNDGKGIPIEIHNKENIYIPEMIFGHLLTSSNYDDDEK 135

  Fly   138 KVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDF-NGTDYTRITFS 201
            |||||||||||||||||||.|.:|||.....:.:.|.|.|||......:|..: .|..||::||.
Yeast   136 KVTGGRNGYGAKLCNIFSTEFILETADLNVGQKYVQKWENNMSICHPPKITSYKKGPSYTKVTFK 200

  Fly   202 PDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDD----D 262
            |||.:|.|..||.||:.:|.||.||:..|.:.::|:|||..|.:||||:|::|::|:.:.    |
Yeast   201 PDLTRFGMKELDNDILGVMRRRVYDINGSVRDINVYLNGKSLKIRNFKNYVELYLKSLEKKRQLD 265

  Fly   263 SGP--------PIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLL 319
            :|.        | .|::|..|.|||||...||..|||:||||||||..||.||:::.|.::|::.
Yeast   266 NGEDGAAKSDIP-TILYERINNRWEVAFAVSDISFQQISFVNSIATTMGGTHVNYITDQIVKKIS 329

  Fly   320 EVLKKKNKGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMS 384
            |:||||.|.  ::|.||::|::::|:|||||||.|.|||||.:|.:.|.|||:|.:..::||.:.
Yeast   330 EILKKKKKK--SVKSFQIKNNMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCEIPLEYINKIM 392

  Fly   385 KSGIVESVLAWAKFKAQNDIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLA 449
            |:.:...:...|....:|.:.|:.|.:.|:|...|||||||:||.|...||||:|||||||.|||
Yeast   393 KTDLATRMFEIADANEENALKKSDGTRKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLA 457

  Fly   450 VSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYG 514
            |:||.|:|||.||.:|||||:||||||:..|:.:||||..:.||:|||::|||   :|.|:||||
Yeast   458 VAGLAVVGRDYYGCYPLRGKMLNVREASADQILKNAEIQAIKKIMGLQHRKKY---EDTKSLRYG 519

  Fly   515 KVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLP-FLEEFITPIVKA--TKKNEE-LSFYSLPEF 575
            .:|||||||.||||||||:|||:.:::|.||.:. ||.||||||:|.  ||..:. ::||::|::
Yeast   520 HLMIMTDQDHDGSHIKGLIINFLESSFPGLLDIQGFLLEFITPIIKVSITKPTKNTIAFYNMPDY 584

  Fly   576 EEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIES 640
            |:|:.:.::..|:..|||||||||.::|.:|||.::|||..:|......|.:.|.:|||||..:.
Yeast   585 EKWREEESHKFTWKQKYYKGLGTSLAQEVREYFSNLDRHLKIFHSLQGNDKDYIDLAFSKKKADD 649

  Fly   641 RKVWLTNHMDEVKRRKELGLPERYLYTKGT------KSITYADFINLELVLFSNADNERSIPSLV 699
            ||.||..                  |..||      |.|..:||||.||:|||.|||.||||:::
Yeast   650 RKEWLRQ------------------YEPGTVLDPTLKEIPISDFINKELILFSLADNIRSIPNVL 696

  Fly   700 DGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLL 764
            ||.|||||||::.|||:|.|.|:|||||:..|:|.:||||||.||..||:.|||||||:|||.||
Yeast   697 DGFKPGQRKVLYGCFKKNLKSELKVAQLAPYVSECTAYHHGEQSLAQTIIGLAQNFVGSNNIYLL 761

  Fly   765 EPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVL 829
            .|.|.||||.:||||.|:||||:|.::.|||.|:||.||||..|..:|.:.:||.|||||:||:|
Yeast   762 LPNGAFGTRATGGKDAAAARYIYTELNKLTRKIFHPADDPLYKYIQEDEKTVEPEWYLPILPMIL 826

  Fly   830 VNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQ 894
            |||||||||||||.|..:||.||:||:|.::|.:|...||||::.:.|.:|.:...||...|.|:
Yeast   827 VNGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQMHPWFRGWTGTIEEIEPLRYRMYGRIE 891

  Fly   895 ILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFER 959
            .:..|.|||:|||...||...||.:|..|| |.:|:|..|.:..|.| |..::|:|:.:|.|..:
Yeast   892 QIGDNVLEITELPARTWTSTIKEYLLLGLS-GNDKIKPWIKDMEEQH-DDNIKFIITLSPEEMAK 954

  Fly   960 IHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLT 1024
              ..:.|||..|||.:.:|...|.|||.:..::::.:..:||.|:|.:|.|||.:|:|.:..:|.
Yeast   955 --TRKIGFYERFKLISPISLMNMVAFDPHGKIKKYNSVNEILSEFYYVRLEYYQKRKDHMSERLQ 1017

  Fly  1025 AQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRG----------YRPDPVKEWQRRIKME 1079
            .:.::.|.|.:||....||:|.|.||.|.|:..||...|          |...|..|...:|...
Yeast  1018 WEVEKYSFQVKFIKMIIEKELTVTNKPRNAIIQELENLGFPRFNKEGKPYYGSPNDEIAEQINDV 1082

  Fly  1080 DAEQADEEDEEEEEAAPSVSSKAKKEKEVD-PEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELL 1143
            ....:||||||        ||....|..:: ||:.:      ..::|||||.:|.||:|:..:||
Yeast  1083 KGATSDEEDEE--------SSHEDTENVINGPEELY------GTYEYLLGMRIWSLTKERYQKLL 1133

  Fly  1144 KQRDTKLSELESLRKKTPEMLWLDDLDALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKG 1208
            ||:..|.:|||:|.|.:.:.:|..||.|.|....|..::: ||.:|.|:..            ||
Yeast  1134 KQKQEKETELENLLKLSAKDIWNTDLKAFEVGYQEFLQRD-AEARGGNVPN------------KG 1185

  Fly  1209 RKVKSMGGGAGAGDVFPDPDGEP------------VEFKITEEIIKKMAAAAKVAQAAKEPKKPK 1261
            .|.|    |.|...:..|.|.:|            .:.|:.::..:::....|:...:|.|.|.|
Yeast  1186 SKTK----GKGKRKLVDDEDYDPSKKNKKSTARKGKKIKLEDKNFERILLEQKLVTKSKAPTKIK 1246

  Fly  1262 EPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGG-----SKTSPKAKK------------AV 1309
            :.|.|.|.:....::..|....|...:  ||...|..     ||.|.|.||            |.
Yeast  1247 KEKTPSVSETKTEEEENAPSSTSSSSI--FDIKKEDKDEGELSKISNKFKKISTIFDKMGSTSAT 1309

  Fly  1310 VKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAKKSDDDVEEVTPR-AERPGRRQASKKIDYSSLF 1373
            .|:...|:.....|:|            :..|||:     .|.|: |::|.|:|  :|:...|..
Yeast  1310 SKENTPEQDDVATKKN------------QTTAKKT-----AVKPKLAKKPVRKQ--QKVVELSGE 1355

  Fly  1374 SDEEEDGGNVGSDDDGNASDDDSPKRPAKRGREDESSGGAKKKAPPKKRRAVIE-SDDDDIEIDE 1437
            ||.|........:|.....||..|    :|.|...||..|  ..|.|.....:| |||..||.||
Yeast  1356 SDLEILDSYTDREDSNKDEDDAIP----QRSRRQRSSRAA--SVPKKSYVETLELSDDSFIEDDE 1414

  Fly  1438 DDDDDSD--FN 1446
            :::..||  ||
Yeast  1415 EENQGSDVSFN 1425

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_001260580.1	PTZ00108	5..1378	CDD:240271	624/1433 (44%)
		HATPase_c	61..>155	CDD:280651	64/93 (69%)
		TopoIIA_Trans_ScTopoIIA	246..398	CDD:239563	71/163 (44%)
		TOPRIM_TopoIIA	435..555	CDD:173785	77/120 (64%)
		TOPRIM_C	553..691	CDD:293503	57/146 (39%)
		TOP4c	691..1172	CDD:238111	219/491 (45%)
TOP2	NP_014311.3	PTZ00108	10..1428	CDD:240271	647/1502 (43%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157344786
Domainoid	1	1.000	396	1.000	Domainoid score	I90
eggNOG	1	0.900	-	-		E1_COG0187
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1110	1.000	Inparanoid score	I20
Isobase	1	0.950	-	0	Normalized mean entropy	S640
OMA	1	1.010	-	-		QHG53902
OrthoFinder	1	1.000	-	-		FOG0002100
OrthoInspector	1	1.000	-	-		oto99458
orthoMCL	1	0.900	-	-		OOG6_100284
Panther	1	1.100	-	-	LDO	PTHR10169
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1536
SonicParanoid	1	1.000	-	-		X836
TreeFam	1	0.960	-	-
	15	14.740