Protein Alignment Top2 and top2b

DIOPT Version :10

• Sequence 1: NP_476760.1
• Gene: Top2 / 35225
• FlyBaseID: FBgn0284220
• Length: 1447
• Species: Drosophila melanogaster
• Sequence 2: XP_002932456.3
• Gene: top2b / 779457
• XenbaseID: XB-GENE-940716
• Length: 1699
• Species: Xenopus tropicalis
• Alignment Length: 1536
• Identity: 831/1536 - (54%)
• Similarity: 1064/1536 - (69%)
• Gaps: 163/1536 - (10%)

  Fly     3 NGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFD 67
            :.:|.:|:|::||||:||||||||||:||||||...:.|||||.... |..::|::|||||||||
 Frog    43 DSSKKMSVERVYQKKTQLEHILLRPDTYIGSVETVTQSMWVYDEDVG-MNCRDITYVPGLYKIFD 106

  Fly    68 EILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNY 132
            ||||||||||||||:||.|||.|||:.|::|:||||:||||..||.:|||||.:|||.|||||||
 Frog   107 EILVNAADNKQRDKNMNCIKISIDPDSNIISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNY 171

  Fly   133 NDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTR 197
            :|:|||||||||||||||||||||.||||||.:|||.||||||.|||.|.::.:||.|:|.|||.
 Frog   172 DDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWTNNMLKTTEPKIKHFDGDDYTC 236

  Fly   198 ITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDD 262
            |||.||||||:|::||:|.|||::|||||:|.|.|||.|.|||.||.|..|:.|:||::|:..|:
 Frog   237 ITFQPDLAKFQMEKLDKDTVALLTRRAYDIAGSCKGVKVLLNGKKLPVNGFRSYVDLYVKDRLDE 301

  Fly   263 SGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNK 327
            :|..:|::||||||||:|....|::||||:||||||||.|||||||:|||.::.:|:||:|||||
 Frog   302 TGVALKVIHEVANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQIVSKLIEVVKKKNK 366

  Fly   328 GGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESV 392
            .|:::|||||:||:||||||||||||||||||||||||.|||||||.|||||....|..|||||:
 Frog   367 AGVSVKPFQVKNHVWVFVNCLIENPTFDSQTKENMTLQPKGFGSKCQLSEKFSKAASNCGIVESI 431

  Fly   393 LAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVI 456
            |.|.|||||..: .|....|.|||||||||:|||:||||:|:.||||||||||||||||||||||
 Frog   432 LNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVI 496

  Fly   457 GRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTD 521
            |||.|||||||||:||||||:.||:.|||||||:.||:||||||.|...:.||||||||:|||||
 Frog   497 GRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTD 561

  Fly   522 QDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHH 586
            |||||||||||||||.|.|||.||:..||||||||||||||..:||||||:|||||||..|.|..
 Frog   562 QDQDGSHIKGLLINFFHHNWPSLLKHSFLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQK 626

  Fly   587 TYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDE 651
            .:.|||||||||||||||||||.|||||||:|:|.|..||.:|.:|||||.::.||.||||.|::
 Frog   627 LWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMED 691

  Fly   652 VKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKR 716
            .::|:..||||::||...||.:||.||||.||:||||:||||||||||||||||||||:||||||
 Frog   692 RRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKR 756

  Fly   717 NDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCA 781
            |||||||||||:|||||||||||||.:|.||||||||||||:||::||:|.|||||||.||||.|
 Frog   757 NDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAA 821

  Fly   782 SARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISN 846
            |.|||||::|||.||:|..:||.||.:..||.|::||.||:||||:|||||||||||||:.|:.|
 Frog   822 SPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQRVEPEWYIPIIPLVLVNGAEGIGTGWACKLPN 886

  Fly   847 YNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVW 911
            |:.|||:.|:::|::|.:|..:.|.||||.|.::.:...:|..:|.|.:|..|.:||:||||..|
 Frog   887 YDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQYAMSGEIYVLDRNTIEITELPVRTW 951

  Fly   912 TQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTT 976
            ||.|||.||||:.|||||...:||:|:||||||||:||:...  |.:...||..|.::||||.|:
 Frog   952 TQVYKEQVLEPMLNGTEKTPSLISDYKEYHTDTTVKFVVKMT--EEKLAQAEAAGLHKVFKLQTS 1014

  Fly   977 LSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKC 1041
            |:.|.|..||...||:::.|..|||||::.||..||..|:::|:|.|.|:|.||::||||:|||.
 Frog  1015 LTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLNYYTLRKEWLIGMLGAEAARLNNQARFVLEKI 1079

  Fly  1042 EKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEK 1106
            :.|:.:|||.::.:...|:::|:..||||.|:     |..|:|.|||:.::   |:..:.:....
 Frog  1080 QGKISIENKTKRDLIQMLVQKGFESDPVKAWK-----EAQEKATEEDDLQD---PNEDNSSDSGT 1136

  Fly  1107 EVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDA 1171
            ...|:           |:|:|.||:|.|::||.:||::|||.|..||..|::|:|..||.:||.|
 Frog  1137 GSGPD-----------FNYILNMSLWSLSKEKVDELVRQRDIKSKELNDLKRKSPPDLWKEDLAA 1190

  Fly  1172 LESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFKI 1236
            ...:|:.||.:|:  |..:.....||:|| |....|.:|::       ..:..|...|..||.:|
 Frog  1191 FVEELDRVEAQEK--EDVLAGFAGKAIKG-KIGKPKMKKLQ-------LEETLPSLYGRRVEPQI 1245

  Fly  1237 TEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGK----QIKAEPDASGDEVDEF-DAMVE 1296
                      :|..|.|:|           |:.|:.||:    .:|.|.|      :|| .|..|
 Frog  1246 ----------SAMKADASK-----------KMLKKKKGEIDSIALKMEFD------EEFSSAPAE 1283

  Fly  1297 GGSK----TSP----KAKKAVVKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAK--------KSD 1345
            ||::    |:|    |.|:.  :||||   ||.||.    :...|....|.|.:        |||
 Frog  1284 GGAEDSMNTTPVKPVKPKRE--RKEPG---PRAKKT----VLTPKSSTKKGKKRNPWSDNESKSD 1339

  Fly  1346 DDVEE------VTPRAERPGRRQASKKIDYSSLFSDEE----EDGGNVG-SDDDGNASDDDSPKR 1399
            .|:||      |.|| :...||.|:::..|:..||:||    :|.|.:. |:::.|.:|...|..
 Frog  1340 SDLEEAEAEPVVIPR-DSLLRRAAAERPKYTFDFSEEEDADDDDEGVISRSNNNNNINDLKPPAS 1403

  Fly  1400 PA--KRGREDE-----------------SSGGAKKKAPPKKRR----------------AVIESD 1429
            ||  .||.:|:                 |:..|:|.:..:|::                |:..||
 Frog  1404 PAANSRGEDDDVLSDSQEKDDYEFSPFKSTPAAQKPSETQKKKDYIFSSSSFSEKVEDSAIYNSD 1468

  Fly  1430 DDD--------------------------IEIDEDDDDDSD 1444
            ||:                          .:.|.|||||.:
 Frog  1469 DDEPSPLYSSYSFKTSEKTTGKGSSKKESAKYDSDDDDDDE 1509

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_476760.1	PTZ00108	5..1378	CDD:240271	805/1404 (57%)
top2b	XP_002932456.3	PTZ00108	45..1464	CDD:240271	820/1487 (55%)
		DTHCT	1591..1687	CDD:462355