DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top2 and top2b

DIOPT Version :9

Sequence 1:	NP_001260580.1	Gene:	Top2 / 35225	FlyBaseID:	FBgn0284220	Length:	1447	Species:	Drosophila melanogaster
Sequence 2:	XP_002932456.3	Gene:	top2b / 779457	XenbaseID:	XB-GENE-940716	Length:	1699	Species:	Xenopus tropicalis

Alignment Length:	1536	Identity:	831/1536 - (54%)
Similarity:	1064/1536 - (69%)	Gaps:	163/1536 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 NGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFD 67
            :.:|.:|:|::||||:||||||||||:||||||...:.|||||.... |..::|::|||||||||
Frog    43 DSSKKMSVERVYQKKTQLEHILLRPDTYIGSVETVTQSMWVYDEDVG-MNCRDITYVPGLYKIFD 106

  Fly    68 EILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNY 132
            ||||||||||||||:||.|||.|||:.|::|:||||:||||..||.:|||||.:|||.|||||||
Frog   107 EILVNAADNKQRDKNMNCIKISIDPDSNIISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNY 171

  Fly   133 NDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTR 197
            :|:|||||||||||||||||||||.||||||.:|||.||||||.|||.|.::.:||.|:|.|||.
Frog   172 DDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWTNNMLKTTEPKIKHFDGDDYTC 236

  Fly   198 ITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDD 262
            |||.||||||:|::||:|.|||::|||||:|.|.|||.|.|||.||.|..|:.|:||::|:..|:
Frog   237 ITFQPDLAKFQMEKLDKDTVALLTRRAYDIAGSCKGVKVLLNGKKLPVNGFRSYVDLYVKDRLDE 301

  Fly   263 SGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNK 327
            :|..:|::||||||||:|....|::||||:||||||||.|||||||:|||.::.:|:||:|||||
Frog   302 TGVALKVIHEVANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQIVSKLIEVVKKKNK 366

  Fly   328 GGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESV 392
            .|:::|||||:||:||||||||||||||||||||||||.|||||||.|||||....|..|||||:
Frog   367 AGVSVKPFQVKNHVWVFVNCLIENPTFDSQTKENMTLQPKGFGSKCQLSEKFSKAASNCGIVESI 431

  Fly   393 LAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVI 456
            |.|.|||||..: .|....|.|||||||||:|||:||||:|:.||||||||||||||||||||||
Frog   432 LNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVI 496

  Fly   457 GRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTD 521
            |||.|||||||||:||||||:.||:.|||||||:.||:||||||.|...:.||||||||:|||||
Frog   497 GRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTD 561

  Fly   522 QDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHH 586
            |||||||||||||||.|.|||.||:..||||||||||||||..:||||||:|||||||..|.|..
Frog   562 QDQDGSHIKGLLINFFHHNWPSLLKHSFLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQK 626

  Fly   587 TYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDE 651
            .:.|||||||||||||||||||.|||||||:|:|.|..||.:|.:|||||.::.||.||||.|::
Frog   627 LWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMED 691

  Fly   652 VKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKR 716
            .::|:..||||::||...||.:||.||||.||:||||:||||||||||||||||||||:||||||
Frog   692 RRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKR 756

  Fly   717 NDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCA 781
            |||||||||||:|||||||||||||.:|.||||||||||||:||::||:|.|||||||.||||.|
Frog   757 NDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAA 821

  Fly   782 SARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISN 846
            |.|||||::|||.||:|..:||.||.:..||.|::||.||:||||:|||||||||||||:.|:.|
Frog   822 SPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQRVEPEWYIPIIPLVLVNGAEGIGTGWACKLPN 886

  Fly   847 YNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVW 911
            |:.|||:.|:::|::|.:|..:.|.||||.|.::.:...:|..:|.|.:|..|.:||:||||..|
Frog   887 YDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQYAMSGEIYVLDRNTIEITELPVRTW 951

  Fly   912 TQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTT 976
            ||.|||.||||:.|||||...:||:|:||||||||:||:...  |.:...||..|.::||||.|:
Frog   952 TQVYKEQVLEPMLNGTEKTPSLISDYKEYHTDTTVKFVVKMT--EEKLAQAEAAGLHKVFKLQTS 1014

  Fly   977 LSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKC 1041
            |:.|.|..||...||:::.|..|||||::.||..||..|:::|:|.|.|:|.||::||||:|||.
Frog  1015 LTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLNYYTLRKEWLIGMLGAEAARLNNQARFVLEKI 1079

  Fly  1042 EKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEK 1106
            :.|:.:|||.::.:...|:::|:..||||.|:     |..|:|.|||:.::   |:..:.:....
Frog  1080 QGKISIENKTKRDLIQMLVQKGFESDPVKAWK-----EAQEKATEEDDLQD---PNEDNSSDSGT 1136

  Fly  1107 EVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDA 1171
            ...|:           |:|:|.||:|.|::||.:||::|||.|..||..|::|:|..||.:||.|
Frog  1137 GSGPD-----------FNYILNMSLWSLSKEKVDELVRQRDIKSKELNDLKRKSPPDLWKEDLAA 1190

  Fly  1172 LESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFKI 1236
            ...:|:.||.:|:  |..:.....||:|| |....|.:|::       ..:..|...|..||.:|
Frog  1191 FVEELDRVEAQEK--EDVLAGFAGKAIKG-KIGKPKMKKLQ-------LEETLPSLYGRRVEPQI 1245

  Fly  1237 TEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGK----QIKAEPDASGDEVDEF-DAMVE 1296
                      :|..|.|:|           |:.|:.||:    .:|.|.|      :|| .|..|
Frog  1246 ----------SAMKADASK-----------KMLKKKKGEIDSIALKMEFD------EEFSSAPAE 1283

  Fly  1297 GGSK----TSP----KAKKAVVKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAK--------KSD 1345
            ||::    |:|    |.|:.  :||||   ||.||.    :...|....|.|.:        |||
Frog  1284 GGAEDSMNTTPVKPVKPKRE--RKEPG---PRAKKT----VLTPKSSTKKGKKRNPWSDNESKSD 1339

  Fly  1346 DDVEE------VTPRAERPGRRQASKKIDYSSLFSDEE----EDGGNVG-SDDDGNASDDDSPKR 1399
            .|:||      |.|| :...||.|:::..|:..||:||    :|.|.:. |:::.|.:|...|..
Frog  1340 SDLEEAEAEPVVIPR-DSLLRRAAAERPKYTFDFSEEEDADDDDEGVISRSNNNNNINDLKPPAS 1403

  Fly  1400 PA--KRGREDE-----------------SSGGAKKKAPPKKRR----------------AVIESD 1429
            ||  .||.:|:                 |:..|:|.:..:|::                |:..||
Frog  1404 PAANSRGEDDDVLSDSQEKDDYEFSPFKSTPAAQKPSETQKKKDYIFSSSSFSEKVEDSAIYNSD 1468

  Fly  1430 DDD--------------------------IEIDEDDDDDSD 1444
            ||:                          .:.|.|||||.:
Frog  1469 DDEPSPLYSSYSFKTSEKTTGKGSSKKESAKYDSDDDDDDE 1509

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_001260580.1	PTZ00108	5..1378	CDD:240271	805/1404 (57%)
		HATPase_c	61..>155	CDD:280651	75/93 (81%)
		TopoIIA_Trans_ScTopoIIA	246..398	CDD:239563	100/151 (66%)
		TOPRIM_TopoIIA	435..555	CDD:173785	97/119 (82%)
		TOPRIM_C	553..691	CDD:293503	90/137 (66%)
		TOP4c	691..1172	CDD:238111	258/480 (54%)
top2b	XP_002932456.3	PTZ00108	45..1464	CDD:240271	820/1487 (55%)
top2b	XP_002932456.3	DTHCT	1591..1686	CDD:400422
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	544	1.000	Domainoid score	I262
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D117851at2759
OrthoFinder	1	1.000	-	-		FOG0002100
OrthoInspector	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10169
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X836
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	5.110