DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top2 and TOP2B

DIOPT Version :9

Sequence 1:	NP_001260580.1	Gene:	Top2 / 35225	FlyBaseID:	FBgn0284220	Length:	1447	Species:	Drosophila melanogaster
Sequence 2:	NP_001317629.1	Gene:	TOP2B / 7155	HGNCID:	11990	Length:	1626	Species:	Homo sapiens

Alignment Length:	1492	Identity:	825/1492 - (55%)
Similarity:	1044/1492 - (69%)	Gaps:	114/1492 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 NGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFD 67
            :.:|.||:|::||||:||||||||||:||||||...:.|||||.... |..:|::||||||||||
Human    44 DSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFD 107

  Fly    68 EILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNY 132
            ||||||||||||||:|..||:.||||.|::|:||||:||||..||.:|:|||.:|||.|||||||
Human   108 EILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNY 172

  Fly   133 NDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTR 197
            :|||||||||||||||||||||||.||||||.:|||.||||||.|||.|.|:.:||.|:|.|||.
Human   173 DDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTC 237

  Fly   198 ITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDD 262
            |||.|||:||||::||:||||||:|||||:|.|.:||.|..||.||.|..|:.|:||::|:..|:
Human   238 ITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDE 302

  Fly   263 SGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNK 327
            :|..:|::||:|||||:|....|::||||:||||||||.|||||||:|||.::.:|:||:|||||
Human   303 TGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNK 367

  Fly   328 GGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESV 392
            .|:::|||||:||:|||:||||||||||||||||||||.|.|||||.|||||....|..|||||:
Human   368 AGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESI 432

  Fly   393 LAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVI 456
            |.|.|||||..: .|....|.|||||||||:|||:||||:|::||||||||||||||||||||||
Human   433 LNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVI 497

  Fly   457 GRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTD 521
            |||.|||||||||:||||||:.||:.|||||||:.||:||||||.|...:.||||||||:|||||
Human   498 GRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTD 562

  Fly   522 QDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHH 586
            |||||||||||||||||.|||.||:..||||||||||||:|..:||||||:|||:|||....|..
Human   563 QDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQK 627

  Fly   587 TYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDE 651
            .:.|||||||||||:|||||||.||:||||||:|.|..||.:|.:|||||.|:.||.||||.|::
Human   628 AWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMED 692

  Fly   652 VKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKR 716
            .::|:..||||::||...||.:||.||||.||:||||:||||||||||||.|||||||:||||||
Human   693 RRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKR 757

  Fly   717 NDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCA 781
            |||||||||||:|||||||||||||.:|.||||||||||||:||||||:|.|||||||.||||.|
Human   758 NDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAA 822

  Fly   782 SARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISN 846
            |.|||||::|.|.||::..:||.||.:..||.|::||.||:|||||||:||||||||||:.|:.|
Human   823 SPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPN 887

  Fly   847 YNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVW 911
            |:.|||:.|:|:|::|.:|..|.|.||||.|.::.:...:|..:|.|.::..|.:||:||||..|
Human   888 YDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTW 952

  Fly   912 TQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTT 976
            ||.|||.||||:.|||:|...:||:|:||||||||:||:...  |.:...||..|.::||||.||
Human   953 TQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMT--EEKLAQAEAAGLHKVFKLQTT 1015

  Fly   977 LSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKC 1041
            |:.|.|..||...||:::.|..|||||::.||..||..|:::|||.|.|::.:|::||||||||.
Human  1016 LTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKI 1080

  Fly  1042 EKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEK 1106
            :.|:.:||:.:|.:...|::|||..||||.|:     |..|:|.||||.:.:...|.|......
Human  1081 QGKITIENRSKKDLIQMLVQRGYESDPVKAWK-----EAQEKAAEEDETQNQHDDSSSDSGTPS- 1139

  Fly  1107 EVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDA 1171
              .|:           |:|:|.||:|.||:||..||:||||.|..|:..|::|:|..||.:||.|
Human  1140 --GPD-----------FNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAA 1191

  Fly  1172 LESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFKI 1236
            ...:|::||.:||  |..:...:.||:|| |....|.:|::       ..:..|.|.|.    :|
Human  1192 FVEELDKVESQER--EDVLAGMSGKAIKG-KVGKPKVKKLQ-------LEETMPSPYGR----RI 1242

  Fly  1237 TEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKG----KQIKAEPDASGDEVDEFD-AMVE 1296
            ..||....|.|:|                 |:.|:.||    ..:|.|.|      :||. |.||
Human  1243 IPEITAMKADASK-----------------KLLKKKKGDLDTAAVKVEFD------EEFSGAPVE 1284

  Fly  1297 GGSKTSPKAKKAVVKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAKK-------SDD------DV 1348
            |..:   :|....|....|.|..|:|||.|..::::.....|..|||       |||      |:
Human  1285 GAGE---EALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDL 1346

  Fly  1349 EE----VTPRAERPGRRQASKKIDYSSLFSDEEEDGGNVGSDDDGNASDDDSPKRPAKRGREDE- 1408
            ||    |.|| :...||.|:::..|:..||:||:|..:...||:.:..:......|.....|||
Human  1347 EETEPVVIPR-DSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEF 1410

  Fly  1409 --SSG---------GAKKKAPPKKR----------------RAVIESDDDDIEIDEDDDDDS 1443
              |.|         ..|.||.|:|.                ....:|:||..:.|.:::|.:
Human  1411 VPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSA 1472

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_001260580.1	PTZ00108	5..1378	CDD:240271	805/1395 (58%)
		HATPase_c	61..>155	CDD:280651	74/93 (80%)
		TopoIIA_Trans_ScTopoIIA	246..398	CDD:239563	97/151 (64%)
		TOPRIM_TopoIIA	435..555	CDD:173785	98/119 (82%)
		TOPRIM_C	553..691	CDD:293503	87/137 (64%)
		TOP4c	691..1172	CDD:238111	263/480 (55%)
TOP2B	NP_001317629.1	Interaction with DNA. /evidence=ECO:0000250	363..365		1/1 (100%)
		Interaction with DNA	1011..1020		5/8 (63%)
		Nuclear export signal	1034..1044		6/9 (67%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1110..1140		10/37 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1274..1604		56/209 (27%)
		Interaction with PLSCR1. /evidence=ECO:0000269\|PubMed:17567603	1506..1512
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	529	1.000	Domainoid score	I286
eggNOG	1	0.900	-	-		E1_COG0187
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1559	1.000	Inparanoid score	I91
Isobase	1	0.950	-	0	Normalized mean entropy	S640
OMA	1	1.010	-	-		QHG53902
OrthoDB	1	1.010	-	-		D117851at2759
OrthoFinder	1	1.000	-	-		FOG0002100
OrthoInspector	1	1.000	-	-		otm41091
orthoMCL	1	0.900	-	-		OOG6_100284
Panther	1	1.100	-	-	O	PTHR10169
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X836
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	14	13.790