Protein Alignment Top2 and TOP2A

DIOPT Version :10

• Sequence 1: NP_476760.1
• Gene: Top2 / 35225
• FlyBaseID: FBgn0284220
• Length: 1447
• Species: Drosophila melanogaster
• Sequence 2: NP_001058.2
• Gene: TOP2A / 7153
• HGNCID: 11989
• Length: 1531
• Species: Homo sapiens
• Alignment Length: 1557
• Identity: 817/1557 - (52%)
• Similarity: 1043/1557 - (66%)
• Gaps: 187/1557 - (12%)

  Fly     2 ENGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIF 66
            |:..|.||:|::||||:||||||||||:||||||...:.|||||.... :..:|::|||||||||
Human    22 EDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIF 85

  Fly    67 DEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSN 131
            ||||||||||||||..|:.|::.||||.|::|:||||:||||..||.:|||||.:|||.||||||
Human    86 DEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSN 150

  Fly   132 YNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYT 196
            |:|||||||||||||||||||||||.||||||:|||||.|||||.:|||:|.::::|.|||.|||
Human   151 YDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYT 215

  Fly   197 RITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDD 261
            .|||.|||:||||..||:||||||.|||||:|.|:|.|.||||||||.|:.|:.|:|:::|:..|
Human   216 CITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLD 280

  Fly   262 DSGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKN 326
            ::|..:|::||..|.||||....|::||||:||||||||.|||||||:|.|.::.:|::|:||||
Human   281 ETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKN 345

  Fly   327 KGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVES 391
            |||:.:|..||:||:|:|||.||||||||||||||||||.|.|||.|.||||||......|||||
Human   346 KGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVES 410

  Fly   392 VLAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGV 455
            :|.|.|||||..: .|....|.::|||||||:|||:|||:||.:||||||||||||:||||||||
Human   411 ILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGV 475

  Fly   456 IGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMT 520
            :|||.|||||||||:||||||:.||:.|||||||:.||:||||||.|..||.||||||||:||||
Human   476 VGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMT 540

  Fly   521 DQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANH 585
            ||||||||||||||||||.|||.|||..|||||||||||.:|..:|::|||||||||||:.|.||
Human   541 DQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNH 605

  Fly   586 HTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMD 650
            ..:.:||||||||||||||||||.||.||||.|||.|..||.:|.:|||||.|:.||.||||.|:
Human   606 KKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFME 670

  Fly   651 EVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFK 715
            :.::||.|||||.|||.:.|..:||.||||.||:||||:||||||||:|||||||||||:|||||
Human   671 DRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFK 735

  Fly   716 RNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDC 780
            ||||||||||||:|||||||:|||||:||.|||:||||||||:||:|||:|.|||||||.||||.
Human   736 RNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDS 800

  Fly   781 ASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKIS 845
            ||.|||||::|.|.||::.|.||..|.:..||.|::||.||:|||||||:|||||||||||.||.
Human   801 ASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIP 865

  Fly   846 NYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGV 910
            |::.|||:.|:|::::|:||..|.|.||||.|.:|.::..:|:.:|.:.||:...:|||||||..
Human   866 NFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRT 930

  Fly   911 WTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTT 975
            |||.|||.||||:.|||||...:|::||||||||||:||:...  |.:...||..|.::||||.|
Human   931 WTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMT--EEKLAEAERVGLHKVFKLQT 993

  Fly   976 TLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEK 1040
            :|:.|.|..||...||:::.|.:|||:::::||.:||..|:::|:|.|.|::.:|::||||||||
Human   994 SLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEK 1058

  Fly  1041 CEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKE 1105
            .:.|:::|||.:|.:...|::|||..||||.|      ::|:|...::||.||:          :
Human  1059 IDGKIIIENKPKKELIKVLIQRGYDSDPVKAW------KEAQQKVPDEEENEES----------D 1107

  Fly  1106 KEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLD 1170
            .|.:.||:.........|:|||.|.:|.||:|||:||.:.|:.|..||::|::|:|..||.:||.
Human  1108 NEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLA 1172

  Fly  1171 ALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFK 1235
            ....:|..||.||:.:||       ..|.| |...|||:|.:       ..:|.|.|.|:.|..:
Human  1173 TFIEELEAVEAKEKQDEQ-------VGLPG-KGGKAKGKKTQ-------MAEVLPSPRGQRVIPR 1222

  Fly  1236 ITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGGSK 1300
            ||.|:                    |...|.|.||:.|.:..:..|...|.|::.....:|
Human  1223 ITIEM--------------------KAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLE---- 1263

  Fly  1301 TSPKAKKAVVKKEPGEKKPRQ-----------KKENGGGLKQSKIDFSKAKAKKSDDDVE-EVTP 1353
                 ||.  |:|||.|..:|           ||.|         .:|.:::.:|.|:.. :|.|
Human  1264 -----KKQ--KREPGTKTKKQTTLAFKPIKKGKKRN---------PWSDSESDRSSDESNFDVPP 1312

  Fly  1354 RAERPGRRQASKKIDYSSLFSDEEEDGGNVGSDDDGNASDDDSP--------------------- 1397
            |...| ||.|:|......|.|||:....:..:||:.....|.||
Human  1313 RETEP-RRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVV 1376

  Fly  1398 ----------------------------------------KRPAKRGREDESSGGAKKKAPPK-- 1420
                                                    |:.|.:.:...|:.||||:|.||
Human  1377 SDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGT 1441

  Fly  1421 ----------------------KRRAVIESDDDDIEID--------------EDDDDDSDFN 1446
                                  ::|....|||.|...:              |.||...||:
Human  1442 KRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFD 1503

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_476760.1	PTZ00108	5..1378	CDD:240271	791/1385 (57%)
TOP2A	NP_001058.2	PTZ00108	25..1497	CDD:240271	812/1546 (53%)
		Interaction with DNA. /evidence=ECO:0000305|PubMed:23022727	342..344		1/1 (100%)
		Interaction with DNA. /evidence=ECO:0000269|PubMed:22841979	990..999		4/8 (50%)
		Nuclear export signal	1018..1028		4/9 (44%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1090..1123		10/48 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1184..1531		80/376 (21%)
		Interaction with PLSCR1. /evidence=ECO:0000269|PubMed:17567603	1433..1439		3/5 (60%)