Protein Alignment Top2 and top2b

DIOPT Version :10

• Sequence 1: NP_476760.1
• Gene: Top2 / 35225
• FlyBaseID: FBgn0284220
• Length: 1447
• Species: Drosophila melanogaster
• Sequence 2: NP_001038656.1
• Gene: top2b / 569877
• ZFINID: ZDB-GENE-041008-136
• Length: 1618
• Species: Danio rerio
• Alignment Length: 1498
• Identity: 830/1498 - (55%)
• Similarity: 1049/1498 - (70%)
• Gaps: 131/1498 - (8%)

  Fly     2 ENGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIF 66
            :..:|.:|:|::||||:||||||||||:||||||...:.|||:| .:..|..:||||||||||||
Zfish    42 KESSKKMSVERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFD-EEIGMNLREISFVPGLYKIF 105

  Fly    67 DEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSN 131
            |||||||||||||||:|.||||.||||.|.:||||||:||||..||::|||||.:||||||||||
Zfish   106 DEILVNAADNKQRDKNMTTIKITIDPESNTISVWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSN 170

  Fly   132 YNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYT 196
            |:|:||||||||||||||||||||..||||||.:|||:||||||..||||:::.:||.|:|.|:|
Zfish   171 YDDNEKKVTGGRNGYGAKLCNIFSLKFTVETACKEYKRSFKQTWTENMGKSNEAKIKYFDGEDFT 235

  Fly   197 RITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDD 261
            .:||.||||||.|::||:|||||::|||||||.|.:||.|.|||.||.|.:|:.|:|.::|:..|
Zfish   236 CVTFQPDLAKFNMEKLDKDIVALLTRRAYDVAGSCRGVKVILNGKKLPVNSFRSYVDFYVKDKLD 300

  Fly   262 DSGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKN 326
            ::|..:|:|:|..||||||....|::||||:||||||||.|||||:|::||.::.:|:||:||||
Zfish   301 ETGVALKVVNETVNERWEVCLTMSEKGFQQISFVNSIATTKGGRHIDYIVDQIVGKLIEVVKKKN 365

  Fly   327 KGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVES 391
            |.|:::|||||:||||||||.||||||||||||||||||.|.|||||.||||||...:..|||||
Zfish   366 KAGVSVKPFQVKNHLWVFVNALIENPTFDSQTKENMTLQTKSFGSKCALSEKFIRAATNCGIVES 430

  Fly   392 VLAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGV 455
            :|.|.|||||..: .|....|.|||||||||:|||:||||:|.:|||||||||||||||||||||
Zfish   431 ILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGV 495

  Fly   456 IGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMT 520
            ||||.|||||||||:||||||..||:.|||||||:.||:||||||.|...:.||:||||::||||
Zfish   496 IGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKILGLQYKKSYDDPESLKSLRYGRIMIMT 560

  Fly   521 DQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANH 585
            ||||||||||||||||.|.|||.||:..||||||||||||:|..:|:||||:|||||||..|.::
Zfish   561 DQDQDGSHIKGLLINFFHHNWPSLLKHTFLEEFITPIVKASKNKQEVSFYSIPEFEEWKKHTESY 625

  Fly   586 HTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMD 650
            .|::|||||||||||||||||||.||:||||:|||:|:.||.:|.:|||||..:.||.||||.|:
Zfish   626 KTWHIKYYKGLGTSTSKEAKEYFSDMERHRIMFKYNGTEDDAAITLAFSKKKTDDRKEWLTNFME 690

  Fly   651 EVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFK 715
            :.::|:..||||:|||...|:.::|.||||.||:||||:||||||||||||||||||||:|||.|
Zfish   691 DRRQRRMHGLPEQYLYGTSTRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCVK 755

  Fly   716 RNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDC 780
            ||||||||||||:|||||||||||||.:|.||||||||||||:||:|:|:|.||||||::||||.
Zfish   756 RNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDA 820

  Fly   781 ASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKIS 845
            ||.|||||::|||.:|::..:|..||.:..||.||:||.||:||||||||||||||||||:.||.
Zfish   821 ASPRYIFTMLSPLAKLLFPAVDSSLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIP 885

  Fly   846 NYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGV 910
            ||:||||:.|:.:|:|.|:|..|.|.||||.|.:..:...:|:.:|.:.:|..|.:|||||||..
Zfish   886 NYDPREIVNNINRMLNHQDPLPMLPSYKNFKGTIHELGQNQYLVSGEVSVLDKNTIEISELPVRT 950

  Fly   911 WTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTT 975
            |||.|||:||||:..||:|...:||:|:||||||||:||:...  |.:...||..|.::||||.:
Zfish   951 WTQAYKESVLEPMLQGTDKTPPLISDYKEYHTDTTVKFVVRMT--EEKLAQAEAAGLHKVFKLQS 1013

  Fly   976 TLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEK 1040
            :|:.|.|..||...|::|:.:..:||||:::||..||..|:|:|||.|.|::.:||:||||:|||
Zfish  1014 SLTCNSMVLFDHMGCMKRYESVQEILKEFFELRLHYYKLRKDWLVGSLGAESAKLSNQARFVLEK 1078

  Fly  1041 CEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKE 1105
            .|.||.:|||.::.:...|::|||..|||..|.:         |.|:..|||:...:.|     :
Zfish  1079 IEGKLSIENKSKRDLIRMLVQRGYESDPVAAWNK---------AQEKSLEEEDGGGNDS-----D 1129

  Fly  1106 KEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLD 1170
            ..|| ..:|..    ..|:|:|.|.:|.||:||..|||||||.|.:||..|:.||.|.||.:||.
Zfish  1130 SSVD-SGSFSG----PNFNYILNMPLWCLTKEKVEELLKQRDLKKAELNELQSKTSENLWREDLA 1189

  Fly  1171 ALESKLNEVEEKERAEEQGINL-KTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEF 1234
            ....:|:.|||:|:   :.:|| |..|.:||:....    |||.|    ...:..|.|.|..||.
Zfish  1190 VFIEELDRVEEQEK---ESVNLGKAVKLVKGKVGKP----KVKKM----QLEETLPSPFGRRVEP 1243

  Fly  1235 KITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGD-EVDEFDAMVEGG 1298
            :||         .|..|.|:|           |:.|:.||     |.||:.. |:|:..|:...|
Zfish  1244 QIT---------LAMKADASK-----------KLTKKKKG-----EIDAAVKLEIDDDSALGSNG 1283

  Fly  1299 SKTS-------------PKAKKAV-VKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAKK------ 1343
            .:.|             |||.:.. .|||||  .|||:|..|          :|..|||
Zfish  1284 GENSLISPSGLPNPGAKPKAPRVKREKKEPG--TPRQRKPAG----------AKGPAKKVKKRNP 1336

  Fly  1344 -SDDD--------------VEEVTPRAERPGRRQASKKIDYSSLFS--DEEEDGGNVGSDDDGNA 1391
             ||||              .|.|.|| :...||.::.|..|:..||  :|||:.|:...::|.:|
Zfish  1337 WSDDDEGGDDDRSDIELDECEPVIPR-DSTSRRASAVKAKYTFDFSEEEEEEEDGDEEQENDASA 1400

  Fly  1392 S---------------DDDSPKRPAKRGREDESSGGAKKKAP----PKKRRAVIESDDDDIEIDE 1437
            |               |||....|.|......:| .||||.|    |.|.....:..|||::.|.
Zfish  1401 SPVHSFKNTEQHDDEVDDDDDIFPPKTTFTLPTS-TAKKKNPEPVLPSKTTYDDKISDDDMKSDS 1464

  Fly  1438 DDD 1440
            |.|
Zfish  1465 DGD 1467

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_476760.1	PTZ00108	5..1378	CDD:240271	805/1412 (57%)
top2b	NP_001038656.1	PTZ00108	45..1376	CDD:240271	801/1401 (57%)
		DTHCT	1510..1605	CDD:462355