Protein Alignment Top2 and Top2b

DIOPT Version :10

• Sequence 1: NP_476760.1
• Gene: Top2 / 35225
• FlyBaseID: FBgn0284220
• Length: 1447
• Species: Drosophila melanogaster
• Sequence 2: NP_001094328.2
• Gene: Top2b / 361100
• RGDID: 1586156
• Length: 1614
• Species: Rattus norvegicus
• Alignment Length: 1487
• Identity: 829/1487 - (55%)
• Similarity: 1044/1487 - (70%)
• Gaps: 109/1487 - (7%)

  Fly     3 NGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFD 67
            :.:|.||:|::||||:||||||||||:||||||...:||||||.... |..:|::||||||||||
  Rat    32 DSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVG-MNCREVTFVPGLYKIFD 95

  Fly    68 EILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNY 132
            ||||||||||||||:|..||:.||||.|::|:||||:||||..||.:|:|||.:|||.|||||||
  Rat    96 EILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNY 160

  Fly   133 NDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTR 197
            :|||||||||||||||||||||||.||||||.:|||.||||||.|||.|.|:.:||.|:|.|||.
  Rat   161 DDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTC 225

  Fly   198 ITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDD 262
            |||.||||||||::||:||||||:|||||:|.|.|||.|..||.||.|..|:.|:||::|:..|:
  Rat   226 ITFQPDLAKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDE 290

  Fly   263 SGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNK 327
            :|..:|::||:|||||:|....|::||||:||||||||.|||||||:|||.::.:|:||:|||||
  Rat   291 TGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNK 355

  Fly   328 GGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESV 392
            .|:::|||||:||:|||:||||||||||||||||||||.|.|||||.|||||....|..|||||:
  Rat   356 AGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESI 420

  Fly   393 LAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVI 456
            |.|.|||||..: .|....|.|||||||||:|||:||||:|::||||||||||||||||||||||
  Rat   421 LNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVI 485

  Fly   457 GRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTD 521
            |||.|||||||||:||||||:.||:.|||||||:.||:||||||.|...:.||||||||:|||||
  Rat   486 GRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTD 550

  Fly   522 QDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHH 586
            |||||||||||||||||.|||.||:..||||||||||||:|..:||||||:|||:|||....|..
  Rat   551 QDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQK 615

  Fly   587 TYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDE 651
            .:.|||||||||||:|||||||.||:||||||:|.|..||.:|.:|||||.|:.||.||||.|::
  Rat   616 AWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMED 680

  Fly   652 VKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKR 716
            .::|:..||||::||...||.:||.||||.||:||||:||||||||||||.|||||||:||||||
  Rat   681 RRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKR 745

  Fly   717 NDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCA 781
            |||||||||||:|||||||||||||.:|.||||||||||||:||||||:|.|||||||.||||.|
  Rat   746 NDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAA 810

  Fly   782 SARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISN 846
            |.|||||::|.|.||::..:||.||.:..||.|::||.||:|||||||:||||||||||:.|:.|
  Rat   811 SPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPN 875

  Fly   847 YNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVW 911
            |:.|||:.|:|:|:.|.:|..|.|.||||.|.::.:...:|..:|.|.::..|.:||:||||..|
  Rat   876 YDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTW 940

  Fly   912 TQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTT 976
            ||.|||.||||:.|||:|...:||:|:||||||||:||:...  |.:...||..|.::||||.||
  Rat   941 TQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMT--EEKLAQAEAAGLHKVFKLQTT 1003

  Fly   977 LSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKC 1041
            |:.|.|..||...||:::.|..|||||::.||..||..|:::|||.|.|::.:|::||||||||.
  Rat  1004 LTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKI 1068

  Fly  1042 EKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAA-PSVSSKAKKE 1105
            :.|:.:||:.:|.:...|::|||..||||.|         ::|.|:..|||:|. |...|.:...
  Rat  1069 QGKITIENRSKKDLIQMLVQRGYESDPVKAW---------KEAQEKAAEEEDAQNPHDDSSSDSG 1124

  Fly  1106 KEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLD 1170
            ....|:           |:|:|.||:|.||:||..||:||||||..|:..|::|:|..||.:||.
  Rat  1125 TPSGPD-----------FNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLA 1178

  Fly  1171 ALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFK 1235
            |...:|::||.:||  |..:...:.||:|| |...||.:|::       ..:..|.|.|..:..:
  Rat  1179 AFVEELDKVEAQER--EDILAGMSGKAVKG-KVGKAKVKKLQ-------LEETMPSPYGRRIVPE 1233

  Fly  1236 ITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGGSK 1300
            ||          |..|.|:::..|.|       |.:|....:|.|.|      :||......|:.
  Rat  1234 IT----------AMKADASRKLLKKK-------KGDPDTTVVKVEFD------EEFSGTPAEGTG 1275

  Fly  1301 TSPKAKKAVVKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAKK-------SDD------DVEE-- 1350
            .......|.|.|  |.|..|:|||.|..::::.....|...||       |||      |:||
  Rat  1276 EETLTPSAPVNK--GPKPKREKKEPGTRVRKTPASTGKPNTKKVKKRNPWSDDESKSESDLEEAE 1338

  Fly  1351 --VTPRAERPGRRQASKKIDYSSLFSDEEEDGGNVGSDDDGNASDDDSPK-RPAKRGREDE---S 1409
              |.|| :...||.|:::..|:..||:||||  :...|||.|..::...| .|.....|||   |
  Rat  1339 PVVIPR-DSLLRRAAAERPKYTFDFSEEEED--DADDDDDNNDLEELKVKASPITNDGEDEFVPS 1400

  Fly  1410 SG---------GAKKKAPPKKR----------------RAVIESDDDDIEIDEDDDD 1441
            .|         ..|.||.|:|.                ....:|:||..:.|.:::|
  Rat  1401 DGIDKDEYAFSPGKSKATPEKSSHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEED 1457

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_476760.1	PTZ00108	5..1378	CDD:240271	805/1391 (58%)
Top2b	NP_001094328.2	PTZ00108	34..1457	CDD:240271	828/1483 (56%)
		DTHCT	1503..1599	CDD:462355