DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top2 and Top2a

DIOPT Version :9

Sequence 1:	NP_001260580.1	Gene:	Top2 / 35225	FlyBaseID:	FBgn0284220	Length:	1447	Species:	Drosophila melanogaster
Sequence 2:	NP_001380697.1	Gene:	Top2a / 360243	RGDID:	62048	Length:	1528	Species:	Rattus norvegicus

Alignment Length:	1515	Identity:	810/1515 - (53%)
Similarity:	1041/1515 - (68%)	Gaps:	136/1515 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 ENGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIF 66
            |:|.|.||:|::||||:||||||||||:||||||...:.|||||.... :..:|::|||||||||
  Rat    21 EDGKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIF 84

  Fly    67 DEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSN 131
            ||||||||||||||..|:.|::.||.|.|::|:||||:||||..||.:|||||.:|||.||||||
  Rat    85 DEILVNAADNKQRDPKMSCIRVTIDAENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSN 149

  Fly   132 YNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYT 196
            |:|||||||||||||||||||||||.||||||:|||||.|||||.:|||:|.|:::|.|:|.|||
  Rat   150 YDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYT 214

  Fly   197 RITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDD 261
            .|||.|||:||||..||:||||||.|||||:|.|:|.|.||||||:|.|:.|:.|:|:::|:..|
  Rat   215 CITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNRLPVKGFRSYVDMYLKDKVD 279

  Fly   262 DSGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKN 326
            ::|..:|:|||..|.||||....|::||||:||||||||.|||||||:|.|.::.:|::|:||||
  Rat   280 ETGNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKN 344

  Fly   327 KGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVES 391
            |||:.:|..||:||:|:|||.||||||||||||||||||.|.|||.|.||||||......|||||
  Rat   345 KGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVES 409

  Fly   392 VLAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGV 455
            :|.|.|||||..: .|....|.::|||||||:|||:||.:||.:||||||||||||:||||||||
  Rat   410 ILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSAECTLILTEGDSAKTLAVSGLGV 474

  Fly   456 IGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMT 520
            :|||.|||||||||:||||||:.||:.|||||||:.||:||||||.|..||.||||||||:||||
  Rat   475 VGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMT 539

  Fly   521 DQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANH 585
            ||||||||||||||||||.|||.|||..|||||||||||.:|..:|::|||||||||||:...||
  Rat   540 DQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSANPNH 604

  Fly   586 HTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMD 650
            ..:.:||||||||||||||||||.:|.||||.|||.|..||.:|.:|||||.::.||.||||.|:
  Rat   605 KKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFME 669

  Fly   651 EVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFK 715
            :.::||.|||||.|||.:.|..:||.||||.||:||||:||||||||:|||||||||||:|||||
  Rat   670 DRRQRKLLGLPEDYLYGQTTMYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFK 734

  Fly   716 RNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDC 780
            ||||||||||||:|||||||:|||||:||.|||:||||||||:||:|||:|.|||||||.||||.
  Rat   735 RNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDS 799

  Fly   781 ASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKIS 845
            ||.|||||::|||.||::...||..|.:..||.|::||.||:|||||||:|||||||||||.||.
  Rat   800 ASPRYIFTMLSPLARLLFPSKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIP 864

  Fly   846 NYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGV 910
            |::.||::.|:|::::|:||..|.|.|||:.|.:|.::..:|:..|.:.||:...:||:|||:..
  Rat   865 NFDVREVVNNIRRLLDGEEPLPMLPSYKNYKGTIEELASNQYVINGEVAILNSTTIEITELPIRT 929

  Fly   911 WTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTT 975
            |||.|||.||||:.|||||...:|::||||||||||:|||...  |.:...||..|.::||||.|
  Rat   930 WTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMT--EEKLAEAERVGLHKVFKLQT 992

  Fly   976 TLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEK 1040
            :|:.|.|..||...||:::.|.:|||:::::||.:||..|:::|:|.|.|::.:|::||||||||
  Rat   993 SLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEK 1057

  Fly  1041 CEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKE 1105
            .:.|:|:|||.:|.:...|::|||..||||.|      ::|:|...|:||.||...|.|..:...
  Rat  1058 IDGKIVIENKPKKELIKVLIQRGYDSDPVKAW------KEAQQKVPEEEENEENEESESESSSPA 1116

  Fly  1106 KEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLD 1170
            .|..|           .|:|||.|.:|.||:|||:||.||||.|..||.:|:||||..||.:||.
  Rat  1117 AESGP-----------TFNYLLDMPLWYLTKEKKDELCKQRDEKEQELNTLKKKTPSDLWKEDLA 1170

  Fly  1171 ALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVEFK 1235
            |...:|..||.||:.:||               ....|:.||:.|..|...:|.|.|.|:    :
  Rat  1171 AFVEELEVVEAKEKQDEQ---------------VGLPGKGVKAKGKKAQISEVLPSPVGK----R 1216

  Fly  1236 ITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGGSK 1300
            :..::..:|.|.|                |.|::::.|.:.::..|...|.|.......:|...|
  Rat  1217 VIPQVTMEMRAEA----------------EKKIRRKIKSENVEGTPAEDGAEPGGLRQRLEKRQK 1265

  Fly  1301 TSP--KAKKAV------VKKEPGEKK--------------------PRQKKENGGGLK------- 1330
            ..|  :|||..      :||  |:||                    ||:|:......|
  Rat  1266 REPGTRAKKQTTLPFKPIKK--GKKKNPWSDSESDMSSNESNFDVPPREKEPRIAATKAKFTADL 1328

  Fly  1331 QSKIDFSKAKAKKSDDD---VEEVTPRAERPGRR-QASKKIDYSSLFSDEEEDG-GNVGSDDDGN 1390
            .|..|||....|..|:|   :::..|:.:.|.:. :.:.|...||...|.|.|| .:|.:....:
  Rat  1329 DSDDDFSGLDEKDEDEDFFPLDDTPPKTKMPPKNTKKALKPQKSSTSVDLESDGKDSVPASPGAS 1393

  Fly  1391 ASD---DDSPKRPAK-----------RGREDESSGGAKKKAPPK--------------------- 1420
            |:|   :..|.:|:.           :|:...|:.|.||:|.||
  Rat  1394 AADVPAETEPSKPSSKQTVGVKRTITKGQSLTSTAGTKKRAVPKETKSDSALNAHVSKKPAPAKA 1458

  Fly  1421 ---KRRAVIESDDDDIEIDE 1437
               ::|....||..|.|.::
  Rat  1459 KNSRKRKPSSSDSSDSEFEK 1478

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_001260580.1	PTZ00108	5..1378	CDD:240271	787/1412 (56%)
		HATPase_c	61..>155	CDD:280651	72/93 (77%)
		TopoIIA_Trans_ScTopoIIA	246..398	CDD:239563	91/151 (60%)
		TOPRIM_TopoIIA	435..555	CDD:173785	99/119 (83%)
		TOPRIM_C	553..691	CDD:293503	87/137 (64%)
		TOP4c	691..1172	CDD:238111	265/480 (55%)
Top2a	NP_001380697.1	PTZ00108	24..1443	CDD:240271	803/1475 (54%)
		Interaction with DNA. /evidence=ECO:0000250\|UniProtKB:P11388	341..343		1/1 (100%)
		Interaction with DNA. /evidence=ECO:0000250\|UniProtKB:P11388	989..998		4/8 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1088..1121		11/38 (29%)
		Interaction with PLSCR1. /evidence=ECO:0000250\|UniProtKB:P11388	1431..1437		3/5 (60%)
		DTHCT	1433..1519	CDD:400422	8/46 (17%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	537	1.000	Domainoid score	I264
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H830
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53902
OrthoDB	1	1.010	-	-		D117851at2759
OrthoFinder	1	1.000	-	-		FOG0002100
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100284
Panther	1	1.100	-	-	LDO	PTHR10169
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X836
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	11	10.930