Protein Alignment Top2 and Top2b

DIOPT Version :10

• Sequence 1: NP_476760.1
• Gene: Top2 / 35225
• FlyBaseID: FBgn0284220
• Length: 1447
• Species: Drosophila melanogaster
• Sequence 2: NP_033435.2
• Gene: Top2b / 21974
• MGIID: 98791
• Length: 1612
• Species: Mus musculus
• Alignment Length: 1497
• Identity: 826/1497 - (55%)
• Similarity: 1039/1497 - (69%)
• Gaps: 129/1497 - (8%)

  Fly     3 NGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFD 67
            :..|.||:|::||||:||||||||||:||||||...:||||||.... |..:|::||||||||||
Mouse    32 DSTKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVG-MNCREVTFVPGLYKIFD 95

  Fly    68 EILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNY 132
            ||||||||||||||:|..||:.||||.|::|:||||:||||..||.:|:|||.:|||.|||||||
Mouse    96 EILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNY 160

  Fly   133 NDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTR 197
            :|||||||||||||||||||||||.||||||.:|||.||||||.|||.|.|:.:||.|:|.|||.
Mouse   161 DDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTC 225

  Fly   198 ITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDD 262
            |||.|||:||||::||:||||||:|||||:|.|.|||.|..||.||.|..|:.|:||::|:..|:
Mouse   226 ITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDE 290

  Fly   263 SGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNK 327
            :|..:|::||:|||||:|....|::||||:||||||||.|||||||:|||.::.:|:||:|||||
Mouse   291 TGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNK 355

  Fly   328 GGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESV 392
            .|:::|||||:||:|||:||||||||||||||||||||.|.|||||.|||||....|..|||||:
Mouse   356 AGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESI 420

  Fly   393 LAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVI 456
            |.|.|||||..: .|....|.|||||||||:|||:||||:|::||||||||||||||||||||||
Mouse   421 LNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVI 485

  Fly   457 GRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTD 521
            |||.|||||||||:||||||:.||:.|||||||:.||:||||||.|...:.||||||||:|||||
Mouse   486 GRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTD 550

  Fly   522 QDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHH 586
            |||||||||||||||||.|||.||:..||||||||||||:|..:||||||:|||:|||....|..
Mouse   551 QDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQK 615

  Fly   587 TYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDE 651
            .:.|||||||||||:|||||||.||:||||||:|.|..||.:|.:|||||.|:.||.||||.|::
Mouse   616 AWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMED 680

  Fly   652 VKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKR 716
            .::|:..||||::||...||.:||.||||.||:||||:||||||||||||.|||||||:||||||
Mouse   681 RRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKR 745

  Fly   717 NDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCA 781
            |||||||||||:|||||||||||||.:|.||||||||||||:||||||:|.|||||||.||||.|
Mouse   746 NDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAA 810

  Fly   782 SARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISN 846
            |.|||||::|.|.||::..:||.||.:..||.|::||.||:|||||||:||||||||||:.|:.|
Mouse   811 SPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPN 875

  Fly   847 YNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVW 911
            |:.|||:.|:|:|:.|.:|..|.|.||||.|.::.:...:|..:|.|.::..|.:||:||||..|
Mouse   876 YDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTW 940

  Fly   912 TQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTT 976
            ||.|||.||||:.|||:|...:||:|:||||||||:||:...  |.:...||..|.::||||.||
Mouse   941 TQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMT--EEKLAQAEAAGLHKVFKLQTT 1003

  Fly   977 LSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKC 1041
            |:.|.|..||...||:::.|..|||||::.||..||..|:::|||.|.|::.:|::||||||||.
Mouse  1004 LTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKI 1068

  Fly  1042 EKKLVVENKQRKAMCDELLKRGYRPDPVKEW---QRRIKMEDAEQADEEDEEEEEAAPSVSSKAK 1103
            :.|:.:||:.:|.:...|::|||..||||.|   |.:...|:..|...:|...:...||
Mouse  1069 QGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPS------ 1127

  Fly  1104 KEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDD 1168
                 .|:           |:|:|.||:|.||:||..||:||||||..|:..|::|:|..||.:|
Mouse  1128 -----GPD-----------FNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKED 1176

  Fly  1169 LDALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVE 1233
            |.|...:|::||.:||  |..:...:.||:|| |....|.:|::       ..:..|.|.|..:.
Mouse  1177 LAAFVEELDKVEAQER--EDILAGMSGKAIKG-KVGKPKVKKLQ-------LEETMPSPYGRRIV 1231

  Fly  1234 FKITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGG 1298
            .:||          |..|.|:::..|.|       |.:|....:|.|.|      :||......|
Mouse  1232 PEIT----------AMKADASRKLLKKK-------KGDPDTTVVKVEFD------EEFSGTPAEG 1273

  Fly  1299 SKTSPKAKKAVVKKEPGEKKPRQKKENGGGLKQSKIDFSKAKAKK-------SDD------DVEE 1350
            :........|.|.|  |.|..|:|||.|..::::.....|..|||       |||      |:||
Mouse  1274 TGEETLTPSAPVNK--GPKPKREKKEPGTRVRKTPTSTGKTNAKKVKKRNPWSDDESKSESDLEE 1336

  Fly  1351 ----VTPRAERPGRRQASKKIDYSSLFSDEEEDGGNVGSDDDGNASDDDS-------PKRPAKRG 1404
                |.|| :...||.|:::..|:..||:||        |||..|:||.:       ...|....
Mouse  1337 AEPVVIPR-DSLLRRAAAERPKYTFDFSEEE--------DDDAAAADDSNDLEELKVKASPITND 1392

  Fly  1405 REDE--------------SSGGAKKKAPPKKR----------------RAVIESDDDDIEIDEDD 1439
            .|||              |||  |.||.|:|.                ....:|:||..:.|.::
Mouse  1393 GEDEFVPSDGLDKDEYAFSSG--KSKATPEKSSNDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNE 1455

  Fly  1440 DD 1441
            :|
Mouse  1456 ED 1457

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_476760.1	PTZ00108	5..1378	CDD:240271	802/1393 (58%)
Top2b	NP_033435.2	PTZ00108	34..1457	CDD:240271	825/1493 (55%)
		Interaction with DNA. /evidence=ECO:0000250	351..353		1/1 (100%)
		Interaction with DNA. /evidence=ECO:0000250	999..1008		5/8 (63%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1098..1128		7/40 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1245..1586		64/239 (27%)
		Interaction with PLSCR1. /evidence=ECO:0000250|UniProtKB:Q02880	1493..1499
		DTHCT	1495..1597	CDD:462355