DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top2 and Top2a

DIOPT Version :9

Sequence 1:	NP_001260580.1	Gene:	Top2 / 35225	FlyBaseID:	FBgn0284220	Length:	1447	Species:	Drosophila melanogaster
Sequence 2:	NP_035753.2	Gene:	Top2a / 21973	MGIID:	98790	Length:	1528	Species:	Mus musculus

Alignment Length:	1567	Identity:	826/1567 - (52%)
Similarity:	1046/1567 - (66%)	Gaps:	182/1567 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 ENGNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIF 66
            |:|.|.||||::||||:||||||||||:||||||...:.|||||.... :..:|::|||||||||
Mouse    21 EDGKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIF 84

  Fly    67 DEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSN 131
            ||||||||||||||..|:.|::.||||.|::|:||||:||||..||.:|:|||.:|||.||||||
Mouse    85 DEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSN 149

  Fly   132 YNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYT 196
            |:|||||||||||||||||||||||.||||||:|||||.|||||.:|||:|.|:::|.|:|.|||
Mouse   150 YDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYT 214

  Fly   197 RITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDD 261
            .|||.|||:||||..||:||||||.|||||:|.|:|.|.||||||.|.|:.|:.|:||::|:..|
Mouse   215 CITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVD 279

  Fly   262 DSGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKN 326
            ::|..:|::||..|.||||....|:|||||:||||||||.|||||||:|.|.::.:|::|:||||
Mouse   280 ETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKN 344

  Fly   327 KGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVES 391
            |||:.:|..||:||:|:|||.||||||||||||||||||.|.|||.|.||||||......|||||
Mouse   345 KGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVES 409

  Fly   392 VLAWAKFKAQNDI-AKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGV 455
            :|.|.|||||..: .|....|.:||||||||:|||:||.:||.:||||||||||||:||||||||
Mouse   410 ILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGV 474

  Fly   456 IGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMT 520
            :|||.|||||||||:||||||:.||:.|||||||:.||:||||||.|..||.||||||||:||||
Mouse   475 VGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMT 539

  Fly   521 DQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANH 585
            ||||||||||||||||||.|||.|||..|||||||||||.:|..:|::|||||||||||:.|.||
Mouse   540 DQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNH 604

  Fly   586 HTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMD 650
            ..:.:||||||||||||||||||.||.||||.|||.|..||.:|.:|||||.::.||.||||.|:
Mouse   605 KKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFME 669

  Fly   651 EVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFK 715
            :.::||.|||||.|||.:.|..:||.||||.||:||||:||||||||:|||||||||||:|||||
Mouse   670 DRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFK 734

  Fly   716 RNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDC 780
            ||||||||||||:|||||||:|||||:||.|||:||||||||:||:|||:|.|||||||.||||.
Mouse   735 RNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDS 799

  Fly   781 ASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKIS 845
            ||.|||||::|||.||::.|.||..|.:..||.|::||.||:|||||||:|||||||||||.||.
Mouse   800 ASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIP 864

  Fly   846 NYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGV 910
            |::.||::.|:|::::|:||..|.|.||||.|.:|.::..:|:..|.:.||....:||||||:..
Mouse   865 NFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRT 929

  Fly   911 WTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTT 975
            |||.|||.||||:.|||||...:|::||||||||||:|||...  |.:...||..|.::||||.:
Mouse   930 WTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMT--EEKLAEAERVGLHKVFKLQS 992

  Fly   976 TLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEK 1040
            :|:.|.|..||...||:::.|.:|||:::::||.:||..|:::|:|.|.|::.:|::||||||||
Mouse   993 SLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEK 1057

  Fly  1041 CEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQ--ADEEDEEEEEAAPSVSSKAK 1103
            .:.|:|:|||.:|.:...|::|||..||||.|      ::|:|  .|||:.||.:...|.|..|
Mouse  1058 IDGKIVIENKPKKELIKVLIQRGYDSDPVKAW------KEAQQKVPDEEENEESDTETSTSDSA- 1115

  Fly  1104 KEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDD 1168
              .|..|           .|:|||.|.:|.||:|||:||.|||:.|..||.:|::|:|..||.:|
Mouse  1116 --AEAGP-----------TFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKED 1167

  Fly  1169 LDALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAKGRKVKSMGGGAGAGDVFPDPDGEPVE 1233
            |.....:|..||.||:.:||       ..|.| |:..|||:|.:.      ..||.|.|.|:.|.
Mouse  1168 LAVFIEELEVVEAKEKQDEQ-------VGLPG-KAGKAKGKKAQM------CADVLPSPRGKRVI 1218

  Fly  1234 FKITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIKAEPDASGDEVDEFDAMVEGG 1298
            .::|.|:                    |...|.|::|:.|.:.::..|...|.|.......:|..
Mouse  1219 PQVTVEM--------------------KAEAEKKIRKKIKSENVEGTPAEDGAEPGSLRQRIEKK 1263

  Fly  1299 SKTSPKAKKAV------VKKEPGEKK--------------------PRQKKENGGGLK------- 1330
            .|..|.|||..      |||  |.||                    ||||::.....|
Mouse  1264 QKKEPGAKKQTTLPFKPVKK--GRKKNPWSDSESDVSSNESNVDVPPRQKEQRSAAAKAKFTVDL 1326

  Fly  1331 QSKIDFSKAKAKKSDDD---VEEVTPRAERPGR--RQASKK-------IDYSSLFSDEEEDGGNV 1383
            .|..|||....|..|:|   ::...|:|:.|.:  ::|.|.       :|..|...|.......|
Mouse  1327 DSDEDFSGLDEKDEDEDFLPLDATPPKAKIPPKNTKKALKTQGSSMSVVDLESDVKDSVPASPGV 1391

  Fly  1384 GSDDDGNASDDDSP-------KRPAKRGREDESSGGAKKKAPPK--------------------- 1420
            .:.|....::...|       |:.|.:.:...|:.|.||:|.||
Mouse  1392 PAADFPAETEQSKPSKKTVGVKKTATKSQSSVSTAGTKKRAAPKGTKSDSALSARVSEKPAPAKA 1456

  Fly  1421 ----KRR-AVIESDDDDIE------------------------------------------IDED 1438
                ||: :..:|.|.|.|                                          ::|.
Mouse  1457 KNSRKRKPSSSDSSDSDFERAISKGATSKKAKGEEQDFPVDLEDTIAPRAKSDRARKPIKYLEES 1521

  Fly  1439 DDDDSDF 1445
            ||||..|
Mouse  1522 DDDDDLF 1528

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_001260580.1	PTZ00108	5..1378	CDD:240271	800/1420 (56%)
		HATPase_c	61..>155	CDD:280651	72/93 (77%)
		TopoIIA_Trans_ScTopoIIA	246..398	CDD:239563	92/151 (61%)
		TOPRIM_TopoIIA	435..555	CDD:173785	99/119 (83%)
		TOPRIM_C	553..691	CDD:293503	89/137 (65%)
		TOP4c	691..1172	CDD:238111	265/482 (55%)
Top2a	NP_035753.2	PTZ00108	24..1441	CDD:240271	812/1475 (55%)
		Interaction with DNA. /evidence=ECO:0000250\|UniProtKB:P11388	341..343		1/1 (100%)
		Interaction with DNA. /evidence=ECO:0000250\|UniProtKB:P11388	989..998		3/8 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1090..1118		10/30 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1183..1211		11/41 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1229..1528		62/300 (21%)
		Interaction with PLSCR1. /evidence=ECO:0000250\|UniProtKB:P11388	1429..1435		3/5 (60%)
		DTHCT	1431..1517	CDD:369680	9/85 (11%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	532	1.000	Domainoid score	I285
eggNOG	1	0.900	-	-		E1_COG0187
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H830
Inparanoid	1	1.050	1560	1.000	Inparanoid score	I93
Isobase	1	0.950	-	0	Normalized mean entropy	S640
OMA	1	1.010	-	-		QHG53902
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002100
OrthoInspector	1	1.000	-	-		otm43161
orthoMCL	1	0.900	-	-		OOG6_100284
Panther	1	1.100	-	-	LDO	PTHR10169
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1536
SonicParanoid	1	1.000	-	-		X836
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.810