DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Top2 and top-2

DIOPT Version :9

Sequence 1:	NP_001260580.1	Gene:	Top2 / 35225	FlyBaseID:	FBgn0284220	Length:	1447	Species:	Drosophila melanogaster
Sequence 2:	NP_496536.1	Gene:	top-2 / 174825	WormBaseID:	WBGene00010785	Length:	1520	Species:	Caenorhabditis elegans

Alignment Length:	1497	Identity:	790/1497 - (52%)
Similarity:	1039/1497 - (69%)	Gaps:	102/1497 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 GNKALSIEQMYQKKSQLEHILLRPDSYIGSVEFT-KELMWVYDNSQNRMVQKEISFVPGLYKIFD 67
            |:|.::||.:|||||||||||||||:||||||.| |..||||:..::::.|::||:|||||||:|
Worm    57 GSKQMAIEDIYQKKSQLEHILLRPDTYIGSVEHTEKTPMWVYNMEESKLEQRDISYVPGLYKIYD 121

  Fly    68 EILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNY 132
            |||||||||||||..||||||.|:.|:|.:||:|||:|||||.||.:|:|||.:|||.|||||||
Worm   122 EILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNY 186

  Fly   133 NDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTR 197
            ||||||||||||||||||||||||.||:||::|:||.:|||||..||.:..:.:|......|:|:
Worm   187 NDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVKSTDEDFTK 251

  Fly   198 ITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDD 262
            ||||||||||||..||:||..||:|||||||.|||||:|||||.::.::.|:||:.::....:::
Worm   252 ITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQFNNE 316

  Fly   263 SGPPIKIVHEVANERWEVACCPSDRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKK-N 326
             |.|:||.:|...:||:||...|::|||||||||||||.|||||||:|.|.::.:.::.:|:| .
Worm   317 -GEPLKIAYEQVGDRWQVALALSEKGFQQVSFVNSIATTKGGRHVDYVADQMVAKFIDSIKRKLT 380

  Fly   327 KGGINIKPFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVES 391
            |..:||||||::||:|||||||||||||||||||.||||||.|||.|.|||||....|..||.::
Worm   381 KTSMNIKPFQIKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDA 445

  Fly   392 VLAWAKFKAQNDIAKTGGR-KSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGV 455
            |::|.:||..:|:.|...: |:||:||||||||||:||.|||.:||||||||||||:||||||.|
Worm   446 VMSWVRFKQMDDLNKKCSKTKTSKLKGIPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSV 510

  Fly   456 IGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMT 520
            :|||.|||||||||||||||.|.||:::|||:|.:.||:||||||||.||||.|||||||:|:|.
Worm   511 VGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMA 575

  Fly   521 DQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANH 585
            ||||||||||||:|||||..||.|::..|:||||||||||||..||:|||||||:.||:.:|.|.
Worm   576 DQDQDGSHIKGLVINFIHHFWPSLIQRNFVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNW 640

  Fly   586 HTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMD 650
            .:|.|||||||||||||||||||.||.||||.|||:|:.||.::.||||||.||.||.||:..|.
Worm   641 KSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMR 705

  Fly   651 EVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFK 715
            |.|.||:.||.|.|||.|.|:.:|:.||:|.|||||||.|||||||.||||.|||||||:|.|||
Worm   706 EKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFK 770

  Fly   716 RNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDC 780
            |.||||||||||:|:|||:|||||||.||..|||||||::||:||||||.|.|||||||.||||.
Worm   771 RADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDS 835

  Fly   781 ASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKIS 845
            ||||||||.:||:||.::...||.:|.:..::.|:|||.||.|||||||||||:||||||||.|.
Worm   836 ASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGIGTGWSTNIP 900

  Fly   846 NYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGV 910
            ||||||::||::::|.|:....:.||||||.|::..:...|:...|.:.:|..|.:||:|||:..
Worm   901 NYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVAVLDDNTIEITELPIKQ 965

  Fly   911 WTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEG-GFYRVFKLT 974
            |||:|||.|||.|...::|...:|.:|:||||||||:||:..:||:...:  |.| ..::||||.
Worm   966 WTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLREL--ERGQDLHQVFKLQ 1028

  Fly   975 TTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILE 1039
            ..::|..|..||...|||.:.:...|.:|:|..|:|.|.:|:::|:|.|.||:.||::||||||.
Worm  1029 AVINTTCMVLFDAAGCLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILA 1093

  Fly  1040 KCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQA-----DEED-----EEEEEA 1094
            |...::|:|||::.|:.|.|:|..:..||||:|:...|:::..::     ||:|     .||:||
Worm  1094 KINNEIVLENKKKAAIVDVLIKMKFDADPVKKWKEEQKLKELRESGEIELDEDDLAAVAVEEDEA 1158

  Fly  1095 APSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKK 1159
               :||.||..:        .||:|   :|||:||::..|:||:||:|:|:.:.|::|:..:.||
Worm  1159 ---ISSAAKAVE--------TKLSD---YDYLVGMALIKLSEEEKNKLIKESEEKMAEVRVIEKK 1209

  Fly  1160 TPEMLWLDDLDALESKLNEVEEKERAEEQGINLKTAKALKGQKSASAK-GRKVKSMGGGAGAGDV 1223
            |.:.||.:|||...|:|::.|.:|:|::.......||.|    :|.|| ||..|.    ....:|
Worm  1210 TWQDLWHEDLDNFVSELDKQEAREKADQDASIKNAAKKL----AADAKTGRGPKK----NVCTEV 1266

  Fly  1224 FPDPDG---EPVEFKITEEIIKKMAAAAKVAQAAKEPKKPKEPKEPKVKKEPKGKQIK------- 1278
            .|..||   ||:....|:...:||:...| .:..||||:|||||  |||||  |:.||
Worm  1267 LPSKDGQRIEPMLDAATKAKYEKMSQPKK-ERVKKEPKEPKEPK--KVKKE--GQDIKKFMSPAA 1326

  Fly  1279 -----------------AEPDASGDEVDEFDAMVEG------GSKTSPKAKKAVVKKE------- 1313
                             .|.|...||..:||:..:|      .||..|:..|...|.|
Worm  1327 PKTAKKEKSDGFNSDMSEESDVEFDEGIDFDSDDDGVEREDVVSKPKPRTGKGAAKAEVIDLSDD 1391

  Fly  1314 ---PGEKKPRQKKENGGGLKQSKIDFSKAKAKKSDDDVEEVTPRAERPGRRQASKKI------DY 1369
               |.:|....||   ...|:.|.:||......||::.|:....:::|..::|:.|.      ..
Worm  1392 DEVPAKKPAPAKK---AAPKKKKSEFSDLSGGDSDEEAEKKPSTSKKPSPKKAAPKTAEPKSKAV 1453

  Fly  1370 SSLFSDEEEDGGN-VGSDDDGNASDDDSPKRPAKRGRED----ESSGGAKKKAPPKKRRAVIESD 1429
            :..|...:::|.. .||||:.:.|...:|:..:.|.|:.    :...|:....|.|||..|::||
Worm  1454 TDFFGASKKNGKKAAGSDDEDDESFVVAPREKSGRARKAPPTYDVDSGSDSDQPKKKRGRVVDSD 1518

  Fly  1430 DD 1431
            .|
Worm  1519 SD 1520

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Top2	NP_001260580.1	PTZ00108	5..1378	CDD:240271	771/1436 (54%)
		HATPase_c	61..>155	CDD:280651	75/93 (81%)
		TopoIIA_Trans_ScTopoIIA	246..398	CDD:239563	85/152 (56%)
		TOPRIM_TopoIIA	435..555	CDD:173785	91/119 (76%)
		TOPRIM_C	553..691	CDD:293503	91/137 (66%)
		TOP4c	691..1172	CDD:238111	248/491 (51%)
top-2	NP_496536.1	PTZ00108	58..1520	CDD:240271	788/1494 (53%)
		HATPase_c	115..>212	CDD:280651	78/96 (81%)
		TopoIIA_Trans_ScTopoIIA	300..452	CDD:239563	85/152 (56%)
		TOPRIM_TopoIIA	490..610	CDD:173785	91/119 (76%)
		TOPRIM_C	608..746	CDD:293503	91/137 (66%)
		TOP4c	746..1222	CDD:238111	248/491 (51%)
		DTHCT	1428..1513	CDD:285332	18/84 (21%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160162463
Domainoid	1	1.000	485	1.000	Domainoid score	I193
eggNOG	1	0.900	-	-		E1_COG0187
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H830
Inparanoid	1	1.050	1483	1.000	Inparanoid score	I43
Isobase	1	0.950	-	0	Normalized mean entropy	S640
OMA	1	1.010	-	-		QHG53902
OrthoDB	1	1.010	-	-		D117851at2759
OrthoFinder	1	1.000	-	-		FOG0002100
OrthoInspector	1	1.000	-	-		oto19744
orthoMCL	1	0.900	-	-		OOG6_100284
Panther	1	1.100	-	-	O	PTHR10169
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1536
SonicParanoid	1	1.000	-	-		X836
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	18	17.750