DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment brat and Trim32

DIOPT Version :9

Sequence 1:	NP_001188842.2	Gene:	brat / 35197	FlyBaseID:	FBgn0010300	Length:	1061	Species:	Drosophila melanogaster
Sequence 2:	NP_001012103.1	Gene:	Trim32 / 313264	RGDID:	1305238	Length:	655	Species:	Rattus norvegicus

Alignment Length:	895	Identity:	160/895 - (17%)
Similarity:	278/895 - (31%)	Gaps:	313/895 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly   188 DAVAKCFECQSYLCANCVTAHE----FMHCFNGHNV---CLIKGFEASTTGTPLSVGSPSNNPAS 245
            ||:.:..||.  :|....|..:    .:||  ||.:   ||.|...:|..|    |..|
  Rat    13 DALREVLECP--ICMESFTEEQLRPKLLHC--GHTICRQCLEKLLASSING----VRCP------ 63

  Fly   246 NEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYGSL 310
                :.|.:|.:....|..|:              ::.|..|..|..::|..
  Rat    64 ----FCSKITRITSLTQLTDN--------------LTVLKIIDTAGLSEAVG------------- 97

  Fly   311 HPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTTST 375
                          .|.|....:.|.:..||:|.:::|:.|...:|          |.||..|..
  Rat    98 --------------LLMCRACGRRLPRQFCRSCGLVLCEPCREADH----------QPPGHCTLP 138

  Fly   376 GSTANE----------SALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQY----QKAHNELNET 426
            ...|.|          :.|:.|..:::.:...:.|::.:.......|..:|    ::...||..:
  Rat   139 VKEAAEERRRDFGEKLTRLRELTGELQRRKAALEGVSRDLQARYKAVLQEYGHEERRVQEELARS 203

  Fly   427 HQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLTEA 491
            .:||...|                |:|..:||                :.||
  Rat   204 RKFFTGSL----------------AEVEKSNS----------------QVVE------------- 223

  Fly   492 LLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNGHGK 556
                                  |..::.|...:||..|..:...:...:|     ::|.
  Rat   224 ----------------------EQSYLLNIAEVQAVSRCDYFLAKIKQAD-----VALL------ 255

  Fly   557 QPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSGASL 621
                                                  .:||..::.:..:|| ...|.|....|
  Rat   256 --------------------------------------EETADEEEPELTASL-PRELTLQDVEL 281

  Fly   622 LDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYNPYE 686
            |        .||                     |.|.|           ..|.|.......|..:
  Rat   282 L--------KVG---------------------HVGPL-----------QIGQAVKKPRTVNMED 306

  Fly   687 KWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVV-SGAGAGGSTVSSESLLDLTNKLLSA 750
            .|          :|..|.:.|::.:..|..:......|| |...:.....|||:...:
  Rat   307 SW----------AVEEGAASSASASVTFREMDMSPEEVVPSPRASPAKQRSSEAASSI------- 354

  Fly   751 TIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEGRFKF 815
                       |:.::..|.|..|...|.|..|..:.|.:|::::|||..|:|||:|:::|..|.
  Rat   355 -----------QQCLFLKKMGAKGSTPGMFNLPVSLYVTSQSEVLVADRGNYRIQVFNRKGFLKE 408

  Fly   816 QFGECGKRDSQLL------YPN--RVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQ 872
            ........||.:|      .||  .::|..|...:|....|..:.:::|...|..|....:. |.
  Rat   409 IRRSPSGIDSFVLSFLGADLPNLTPLSVAMNCHGLIGVTDSYDNSLKVYTLDGHCVACHRSQ-LS 472

  Fly   873 HPRGVTVDNKGRIIVVECKVMRVIIF--DQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFIS---- 931
            .|.|:|....|:.:|.:.:..::..|  |:...|: |:.|......|..|..:.:..::.:
  Rat   473 KPWGITALPSGQFVVTDVEGGKLWCFTVDRGAGVV-KYSCLCSAVRPKFVTCDAEGTVYFTQGLG 536

  Fly   932 ------DNRAHCVKVFNY-----EGQYLRQIGGEGITNYPI----GVGINSNGEILIADNHNNFN 981
                  .|..|....|:.     :||..|||......|...    |:.:::.|::::||:.....
  Rat   537 LNVENRQNEHHLEGGFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEI 601

  Fly   982 LTIFTQDGQLISALESKVKHAQC-FDVALMDDGS-VVLASKDYRLYIYRY 1029
            |......|..:...|.    ..| ..:||...|. :||...|:.:.||.|
  Rat   602 LHFPKGGGYSVLIREG----LTCPVGIALTPKGQLLVLDCWDHCVKIYSY 647

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
brat	NP_001188842.2	BBOX	176..222	CDD:197662	11/40 (28%)
		zf-B_box	328..366	CDD:279037	8/37 (22%)
		NHL_brat_like	760..1030	CDD:271329	70/301 (23%)
		NHL repeat	783..819	CDD:271329	13/35 (37%)
		NHL repeat	830..871	CDD:271329	9/42 (21%)
		NHL repeat	874..910	CDD:271329	9/37 (24%)
		NHL repeat	914..954	CDD:271329	10/54 (19%)
		NHL repeat	959..997	CDD:271329	6/41 (15%)
		NHL repeat	1002..1027	CDD:271329	7/26 (27%)
Trim32	NP_001012103.1	RING-HC_TRIM32_C-VII	20..66	CDD:319501	15/63 (24%)
		Bbox1_TRIM32_C-VII	99..139	CDD:380864	13/49 (27%)
		NHL_TRIM32_like	364..646	CDD:271331	66/287 (23%)
		NHL repeat	376..416	CDD:271331	13/39 (33%)
		NHL repeat	433..472	CDD:271331	7/39 (18%)
		NHL repeat	474..514	CDD:271331	10/40 (25%)
		NHL repeat	515..566	CDD:271331	7/50 (14%)
		NHL repeat	579..611	CDD:271331	6/31 (19%)
		NHL repeat	619..645	CDD:271331	7/25 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D489543at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.920