DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment brat and ncl-1

DIOPT Version :9

Sequence 1:	NP_001188842.2	Gene:	brat / 35197	FlyBaseID:	FBgn0010300	Length:	1061	Species:	Drosophila melanogaster
Sequence 2:	NP_001299853.1	Gene:	ncl-1 / 176084	WormBaseID:	WBGene00003559	Length:	982	Species:	Caenorhabditis elegans

Alignment Length:	999	Identity:	385/999 - (38%)
Similarity:	507/999 - (50%)	Gaps:	239/999 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    64 VTVINGH-HP------------ISASVSSSSSASSSSCSSSSSSSSSSSSSSSSTSGLSGCGSTS 115
            :.|.|.| ||            :.....|.||.:|||...|:|.:......:..|:.:.|
Worm   176 IPVPNAHLHPSMVAGSDPSNPVVGFGFGSPSSTTSSSPPLSNSPTIEQQQHAQLTAMMQG----- 235

  Fly   116 SSVISANNVASSNGPGVIGSNLQSSNNGGNSGISSLVVGAGKGSNSSSNSSSSNTSANGSPPRCT 180
              ::|.||||.|||.||..:::.:.:                                     |:
Worm   236 --IMSNNNVAVSNGSGVQVASVPAVH-------------------------------------CS 261

  Fly   181 ACKSKCS--DAVAKCFECQSYLCANCVTAHEFMHCFNGHNVCLIKGFEASTTGTPLSVGSPSNNP 243
            .|||..:  .|.:.|.:|.:.||.||..||:|||||..|.|..:     :|.||    ||.|::.
Worm   262 GCKSNETKLQATSFCQDCNANLCDNCTMAHKFMHCFADHRVVSL-----TTPGT----GSSSSST 317

  Fly   244 ASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQREDSIYG 308
            :|                                              ::.|:|....:..|:.|
Worm   318 SS----------------------------------------------SSSASSTSSHQVPSLGG 336

  Fly   309 SLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQSPGMTT 373
            ...|  ........:|.:.|.:|:...|.|.|.:|.:.:|::|.|.:|.:|.|:.|.:
Worm   337 KQSP--DSMMLGSGKRSVLCLQHRASELVFFCVSCNLAICRDCTVSDHPSGTHQYELI------- 392

  Fly   374 STGSTANESAL--QTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELNETHQFFASMLDE 436
              ...|::..|  :.|:||.|.|..:::.:....|.....:......||.:|.||.....:::.:
Worm   393 --ADVADKQMLKMEQLIADARSKHADMLDMFKQVDNKQQVLTASLHNAHAQLEETVSNLINVIQD 455

  Fly   437 RKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVER-----SPAPPSSLLTEALLLRK 496
            :|..|.|:::..:.||........:|.:.:.||...|.|...|     |||       |.::.::
Worm   456 QKKTLAKDIDNAFAAKQIQLTMVDKRIQSMADKLSQTIEFSRRLMSFASPA-------EVMVFKQ 513

  Fly   497 SLEQQLQ---------TGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSN 552
            .|:.:||         :|:  :..|.|||::.     .||:.|       ||:.   |...|..:
Worm   514 LLDTRLQLFLGFNPDTSGV--LMTPCEIEYLG-----AAGLFN-------NSAS---TVSQLLGS 561

  Fly   553 GHGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLS 617
            .||..|....|  :|::.....||....|..|.         ......|...|:|..|.:...|
Worm   562 VHGGSPINNAP--AANDFLMPQAGLAPIGRAQS---------RIIPIEQNQLARSPPHHIAGSL- 614

  Fly   618 GASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHY 682
                         .:.|:|:..||...:|         ||......|.....|::....:|....
Worm   615 -------------PMNAYSDSNLLRPNKD---------FGGSSQSLGPFNPLGASVPGAAADPFS 657

  Fly   683 NPYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKL 747
            :.|:|||            .||..|            |||.:      .|..|..|..
Worm   658 SQYDKWS------------LGVEPS------------VGGLL------EGGNVDEEKF------- 685

  Fly   748 LSATIYPP-KSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEG 811
              .|::|| :|||||||||||||||||||||||||||||||||.|.||:||||||||||:|||||
Worm   686 --QTLFPPSRSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNGQGDIVVADTNNHRIQVFDKEG 748

  Fly   812 RFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPTHQIQIYNQYGQFVRKFGATILQHPRG 876
            |||||||||||||.||||||||||.|.:||.:|||||||||||:|||||||:|||||.|||||||
Worm   749 RFKFQFGECGKRDGQLLYPNRVAVNRTTGDFVVTERSPTHQIQVYNQYGQFLRKFGANILQHPRG 813

  Fly   877 VTVDNKGRIIVVECKVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVVNDKQEIFISDNRAHCVKVF 941
            |.||:||||||||||||||||||..||:|.||.||::|||||||..|||.||.||||||||:|||
Worm   814 VCVDSKGRIIVVECKVMRVIIFDMFGNILQKFSCSRYLEFPNGVCTNDKNEILISDNRAHCIKVF 878

  Fly   942 NYEGQYLRQIGGEGITNYPIGVGINSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFD 1006
            :|||||||||||||:|||||||||||.||:::||||||||||:|:|||.:|.||||:||||||||
Worm   879 SYEGQYLRQIGGEGVTNYPIGVGINSLGEVVVADNHNNFNLTVFSQDGTMIGALESRVKHAQCFD 943

  Fly  1007 VALMDDGSVVLASKDYRLYIYRYV 1030
            |||:|||||||||||||||:||::
Worm   944 VALVDDGSVVLASKDYRLYLYRFL 967

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
brat	NP_001188842.2	BBOX	176..222	CDD:197662	20/47 (43%)
		zf-B_box	328..366	CDD:279037	13/37 (35%)
		NHL_brat_like	760..1030	CDD:271329	228/269 (85%)
		NHL repeat	783..819	CDD:271329	31/35 (89%)
		NHL repeat	830..871	CDD:271329	32/40 (80%)
		NHL repeat	874..910	CDD:271329	29/35 (83%)
		NHL repeat	914..954	CDD:271329	33/39 (85%)
		NHL repeat	959..997	CDD:271329	28/37 (76%)
		NHL repeat	1002..1027	CDD:271329	23/24 (96%)
ncl-1	NP_001299853.1	RING	87..131	CDD:238093
		BBOX	256..305	CDD:197662	20/85 (24%)
		BBOX	349..392	CDD:197662	14/42 (33%)
		BBC	399..525	CDD:128778	30/132 (23%)
		NHL_brat_like	697..967	CDD:271329	228/269 (85%)
		NHL repeat	720..756	CDD:271329	31/35 (89%)
		NHL repeat	767..808	CDD:271329	32/40 (80%)
		NHL repeat	811..847	CDD:271329	29/35 (83%)
		NHL repeat	851..891	CDD:271329	33/39 (85%)
		NHL repeat	896..934	CDD:271329	28/37 (76%)
		NHL repeat	939..964	CDD:271329	23/24 (96%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160166668
Domainoid	1	1.000	80	1.000	Domainoid score	I5558
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	573	1.000	Inparanoid score	I570
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45494
OrthoDB	1	1.010	-	-		D133242at33208
OrthoFinder	1	1.000	-	-		FOG0016649
OrthoInspector	1	1.000	-	-		oto19850
orthoMCL	1	0.900	-	-		OOG6_113938
Panther	1	1.100	-	-	LDO	PTHR24104
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2677
SonicParanoid	1	1.000	-	-		X13516
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	12	12.030