DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment brat and trim32

DIOPT Version :9

Sequence 1:	NP_001188842.2	Gene:	brat / 35197	FlyBaseID:	FBgn0010300	Length:	1061	Species:	Drosophila melanogaster
Sequence 2:	NP_001096393.1	Gene:	trim32 / 100124994	XenbaseID:	XB-GENE-1011959	Length:	649	Species:	Xenopus tropicalis

Alignment Length:	890	Identity:	158/890 - (17%)
Similarity:	254/890 - (28%)	Gaps:	371/890 - (41%)

- Green bases have known domain annotations that are detailed below.

  Fly   188 DAVAKCFECQSYLCANCVTAH----EFMHCFNGHNVCL----------IKGFEASTTGTPLSVGS 238
            ||:.:..||.  :|....|..    :.:.|  ||..|.          |.|...........|.|
Frog    10 DALREVLECP--ICMEAYTEELLRPKLLQC--GHTTCTQCLEKLLASNINGVRCPFCSRVTRVTS 70

  Fly   239 PSNNPASNEFKYASSLTMMLQQQQQLDSQQQQQQQQLLPAQPMSQLSKIVLAAAAQANSQEQQRE 303
            .:|        .|.:||::                            ||:       |:.|..:
Frog    71 LAN--------LADNLTVL----------------------------KII-------NTSELGQ- 91

  Fly   304 DSIYGSLHPQQQQQQQQQQQRQLFCPRHKQELLKFSCRTCCILVCKECIVLEHSTGLHELENVQS 368
             ::.|                 |.|...|:.|.:|.|:.|.:.:|..|...:|....|.:.::|
Frog    92 -TVIG-----------------LMCKSCKRRLPRFFCKNCIVSLCDACKEADHVLLNHIVLSLQ- 137

  Fly   369 PGMTTSTGSTANE---------SALQTLLADMRGKIGEIVGIAGNSDQNLTKVKLQYQKAHNELN 424
                    ..|||         :.|:..:.|::.:...:.|:.       ::||.:|:
Frog   138 --------DAANERRKTLEERLAKLRQCMQDLQKRKSILEGLT-------SQVKCKYK------- 180

  Fly   425 ETHQFFASMLDERKTELLKELETLYTAKVNSNNSWQQRSRDLIDKGLATCEAVERSPAPPSSLLT 489
                   |:|.:...|..:..|.|            .|||.:....|:..|.:.      |.:|.
Frog   181 -------SILQDYSKEECRVQEEL------------ARSRKMFTASLSEVEKLN------SQMLD 220

  Fly   490 E-ALLLRKSLEQQLQTGIQEMQLPFEIEFMSNYQSIQAGVRNTFGYIRANSSDGGPTGMSLTSNG 553
            | |.||          .|.|:||....:::               .::...||......||...
Frog   221 EQAYLL----------NITEVQLVSRCDYI---------------LLKIKQSDTALLEDSLEEE- 259

  Fly   554 HGKQPPIARPTQSASNSSASSAGSGHHGHHQQSHHHGHHNHHQTAHHQQLQAQSSLHGLGLGLSG 618
                   |.|..:|..:|.
Frog   260 -------AEPELAADLTSE---------------------------------------------- 271

  Fly   619 ASLLDSSSSAGGAVGAFSNGGLLLGGRDRNALAVEQHFGELMPKRGGGGYTGSNGSATSAVAHYN 683
            .:|.|......|..|....|.::     :....|...  |.:|:           .:|.....|.
Frog   272 LTLQDPEFLKVGHAGPIQVGQVV-----KKPYIVNMQ--EPLPE-----------VSTFTTISYK 318

  Fly   684 PYEKWSNGGSDNLFSSVTSGVSGSSAVADAFASLSAVGGSVVSGAGAGGSTVSSESLLDLTNKLL 748
            ..:..|..     .|||.|..|         |.|:.:
Frog   319 EMDMQSEE-----ISSVQSCPS---------APLTKI---------------------------- 341

  Fly   749 SATIYPPKSQIKRQKMIYHCKFGEFGVMEGQFTEPSGVAVNAQNDIIVADTNNHRIQIFDKEG-- 811
                  |::....|:..:..|.|..|.|.|.|..|..:.|.|..:::|||..|.|||:|:::|
Frog   342 ------PEASCNLQQCQFLRKMGSKGNMPGMFNLPVSLHVTALGEVLVADRGNCRIQVFNRKGFL 400

  Fly   812 -----------RFKFQF------------------GECGKRDSQLLYPNRVAVVRNSGDIIVTER 847
                       .|...|                  |..|..|:   |.|.|.|....|..|...|
Frog   401 REIRRSPSGIDTFVLSFLGADLPNLIPLSVTMNCHGLIGVTDN---YDNSVKVYTMEGHCIACHR 462

  Fly   848 SPTHQIQIYNQYGQFVRKFGATILQHPRG-----VTVDNKGRIIVVE--CKVMR--VIIFDQNGN 903
            |     |:...:|......|..::....|     :|||....::...  |..:|  .:..||.|.
Frog   463 S-----QLSKPWGIAAMPSGQFVVTDVEGGKLWCLTVDRSLGVVKYSRLCNAVRPKFVTCDQEGT 522

  Fly   904 VLHKFGCSKHLE-----------FPNGVVVNDKQ---------------------------EIFI 930
            |....|...:||           |..|.|.:|.|                           ::.:
Frog   523 VYFTQGLGLNLENRQFEHHLEGGFSIGSVGSDGQLGRQISHFFSENEDFRCIAGMCVGARGDLIV 587

  Fly   931 SDNRAHCVKVFNYEGQYLRQIGGEGITNYPIGVGINSNGEILIAD 975
            :|:....:..|...|.|...| .||:| .|:||.:...|::|:.|
Frog   588 ADSGRKEILHFPKSGGYSILI-REGLT-CPVGVSVTPKGQLLVLD 630

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
brat	NP_001188842.2	BBOX	176..222	CDD:197662	10/47 (21%)
		zf-B_box	328..366	CDD:279037	10/37 (27%)
		NHL_brat_like	760..1030	CDD:271329	67/294 (23%)
		NHL repeat	783..819	CDD:271329	14/66 (21%)
		NHL repeat	830..871	CDD:271329	10/40 (25%)
		NHL repeat	874..910	CDD:271329	10/44 (23%)
		NHL repeat	914..954	CDD:271329	12/77 (16%)
		NHL repeat	959..997	CDD:271329	6/17 (35%)
		NHL repeat	1002..1027	CDD:271329
trim32	NP_001096393.1	RING-HC_TRIM32_C-VII	17..63	CDD:319501	10/49 (20%)
		RING-HC finger (C3HC4-type)	18..62	CDD:319501	9/47 (19%)
		Bbox_SF	96..136	CDD:381767	11/39 (28%)
		NHL_TRIM32_like	358..640	CDD:271331	65/283 (23%)
		NHL repeat	370..410	CDD:271331	12/39 (31%)
		NHL repeat	427..466	CDD:271331	12/46 (26%)
		NHL repeat	468..507	CDD:271331	6/38 (16%)
		NHL repeat	509..560	CDD:271331	13/50 (26%)
		NHL repeat	573..605	CDD:271331	3/31 (10%)
		NHL repeat	613..639	CDD:271331	7/19 (37%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D489543at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.920