DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment mib2 and Sqstm1

DIOPT Version :9

Sequence 1:	NP_001260554.1	Gene:	mib2 / 35170	FlyBaseID:	FBgn0086442	Length:	1049	Species:	Drosophila melanogaster
Sequence 2:	NP_787037.2	Gene:	Sqstm1 / 113894	RGDID:	69287	Length:	439	Species:	Rattus norvegicus

Alignment Length:	337	Identity:	75/337 - (22%)
Similarity:	115/337 - (34%)	Gaps:	129/337 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly    82 HSNVVCDGCSKAGIAGIVFKCAQCPNYHLCAYCYAEDLHDIEHPFIRYTTPNSLGVRLPMRKGAK 146
            |.||:||||: ..:.|..:||:.||:|.||:.|..:.||. ||..:.:  ||..|
  Rat   120 HPNVICDGCN-GPVVGTRYKCSVCPDYDLCSVCEGKGLHR-EHSKLIF--PNPFG---------- 170

  Fly   147 RIQLRGIFVGSKVVR--------GPDWE------WNEQD--GGEGRTGRVMEIRGWDNESCRSVA 195
              .|...|..|:.:|        .|.||      |:.:.  .|:||.....|.....:|.    .
  Rat   171 --HLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPSED----P 229

  Fly   196 NVAWVTG-----STNVYRLGHKGNVDLKY------ITAT-------------------CGGHYYK 230
            ||.::..     :..:..||.:.::|:::      :|.|                   |.....|
  Rat   230 NVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTSAESSSTGTEDKSGTQPSSCSSEVSK 294

  Fly   231 ----DHMPVLGQTEEL---------QPVAPMVKPSFSVGDRVKVCL---EVD------------- 266
                ...|....||::         ||...|...:.|.||.....|   |||
  Rat   295 PDGAGEGPAQSLTEQMKKIALESVGQPEELMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPE 359

  Fly   267 -----ALMKLQQGHGGW-------------NPRMVEHLSKL---------GTVHRI--TDKGDI- 301
                 :|...|:|..|.             :||::|.||::         |.:.|:  |...||
  Rat   360 SEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIG 424

  Fly   302 ----RVQYENCP 309
                .:||...|
  Rat   425 AALDTIQYSKHP 436

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mib2	NP_001260554.1	MIB_HERC2	8..73	CDD:284184
		ZZ_Mind_bomb	85..129	CDD:239079	18/43 (42%)
		MIB_HERC2	156..220	CDD:284184	16/90 (18%)
		ANK	464..582	CDD:238125
		ANK repeat	464..494	CDD:293786
		Ank_2	468..560	CDD:289560
		ANK repeat	496..527	CDD:293786
		ANK repeat	529..560	CDD:293786
		Ank_5	549..604	CDD:290568
		ANK	557..685	CDD:238125
		ANK repeat	596..628	CDD:293786
		Ank_2	601..695	CDD:289560
		ANK repeat	630..662	CDD:293786
		ANK repeat	664..695	CDD:293786
		zf-C3HC4_3	918..961	CDD:290631
		zf-C3HC4_3	1002..1044	CDD:290631
Sqstm1	NP_787037.2	Interaction with LCK. /evidence=ECO:0000250\|UniProtKB:Q13501	2..48
		PB1_p62	4..100	CDD:99723
		Interaction with PRKCZ and dimerization. /evidence=ECO:0000269\|PubMed:10477520, ECO:0000269\|PubMed:12431995	41..105
		Interaction with PAWR. /evidence=ECO:0000250\|UniProtKB:Q13501	48..78
		Interaction with GABRR3. /evidence=ECO:0000269\|PubMed:12431995	119..221		34/116 (29%)
		ZZ_NBR1_like	123..163	CDD:239080	18/41 (44%)
		LIM protein-binding. /evidence=ECO:0000250\|UniProtKB:Q13501	167..217		13/61 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	201..231		6/33 (18%)
		TRAF6-binding. /evidence=ECO:0000250	225..230		1/8 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	259..389		21/129 (16%)
		Interaction with NTRK1. /evidence=ECO:0000269\|PubMed:11244088	266..439		34/171 (20%)
		MAP1LC3B-binding. /evidence=ECO:0000250\|UniProtKB:Q13501	320..341		6/20 (30%)
		LIR	335..340		0/4 (0%)
		Interaction with KEAP1. /evidence=ECO:0000250\|UniProtKB:Q64337	346..351		1/4 (25%)
		UBA_5	378..439	CDD:318725	13/59 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4582
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900