Protein Alignment mib2 and Sqstm1

DIOPT Version :10

• Sequence 1: NP_609933.1
• Gene: mib2 / 35170
• FlyBaseID: FBgn0086442
• Length: 1049
• Species: Drosophila melanogaster
• Sequence 2: NP_787037.2
• Gene: Sqstm1 / 113894
• RGDID: 69287
• Length: 439
• Species: Rattus norvegicus
• Alignment Length: 337
• Identity: 75/337 - (22%)
• Similarity: 115/337 - (34%)
• Gaps: 129/337 - (38%)

  Fly    82 HSNVVCDGCSKAGIAGIVFKCAQCPNYHLCAYCYAEDLHDIEHPFIRYTTPNSLGVRLPMRKGAK 146
            |.||:||||: ..:.|..:||:.||:|.||:.|..:.||. ||..:.:  ||..|
  Rat   120 HPNVICDGCN-GPVVGTRYKCSVCPDYDLCSVCEGKGLHR-EHSKLIF--PNPFG---------- 170

  Fly   147 RIQLRGIFVGSKVVR--------GPDWE------WNEQD--GGEGRTGRVMEIRGWDNESCRSVA 195
              .|...|..|:.:|        .|.||      |:.:.  .|:||.....|.....:|.    .
  Rat   171 --HLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPSED----P 229

  Fly   196 NVAWVTG-----STNVYRLGHKGNVDLKY------ITAT-------------------CGGHYYK 230
            ||.::..     :..:..||.:.::|:::      :|.|                   |.....|
  Rat   230 NVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTSAESSSTGTEDKSGTQPSSCSSEVSK 294

  Fly   231 ----DHMPVLGQTEEL---------QPVAPMVKPSFSVGDRVKVCL---EVD------------- 266
                ...|....||::         ||...|...:.|.||.....|   |||
  Rat   295 PDGAGEGPAQSLTEQMKKIALESVGQPEELMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPE 359

  Fly   267 -----ALMKLQQGHGGW-------------NPRMVEHLSKL---------GTVHRI--TDKGDI- 301
                 :|...|:|..|.             :||::|.||::         |.:.|:  |...||
  Rat   360 SEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIG 424

  Fly   302 ----RVQYENCP 309
                .:||...|
  Rat   425 AALDTIQYSKHP 436

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mib2	NP_609933.1	MIB_HERC2	8..74	CDD:461991
		ZZ_Mind_bomb	85..129	CDD:239079	18/43 (42%)
		MIB_HERC2	156..220	CDD:461991	16/90 (18%)
		SH3_15	253..319	CDD:465720	24/107 (22%)
		SH3_15	325..391	CDD:465720
		ANKYR	398..664	CDD:440430
		ANK repeat	464..494	CDD:293786
		ANK repeat	496..527	CDD:293786
		ANK repeat	529..560	CDD:293786
		ANK repeat	596..628	CDD:293786
		ANK repeat	630..662	CDD:293786
		Ank_5	650..705	CDD:433530
		ANK repeat	664..695	CDD:293786
		PRK14959	<791..>919	CDD:184923
		RING-HC_MIB2_rpt1	918..955	CDD:438386
		RING-HC_MIBs-like	1004..1042	CDD:438183
Sqstm1	NP_787037.2	Interaction with LCK. /evidence=ECO:0000250|UniProtKB:Q13501	2..48
		PB1_p62	4..100	CDD:99723
		Interaction with PRKCZ and dimerization. /evidence=ECO:0000269|PubMed:10477520, ECO:0000269|PubMed:12431995	41..105
		Interaction with PAWR. /evidence=ECO:0000250|UniProtKB:Q13501	48..78
		Interaction with GABRR3. /evidence=ECO:0000269|PubMed:12431995	119..221		34/116 (29%)
		ZZ_NBR1_like	123..163	CDD:239080	18/41 (44%)
		LIM protein-binding. /evidence=ECO:0000250|UniProtKB:Q13501	167..217		13/61 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	201..231		6/33 (18%)
		TRAF6-binding. /evidence=ECO:0000250	225..230		1/8 (13%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	259..389		21/129 (16%)
		Interaction with NTRK1. /evidence=ECO:0000269|PubMed:11244088	266..439		34/171 (20%)
		MAP1LC3B-binding. /evidence=ECO:0000250|UniProtKB:Q13501	320..341		6/20 (30%)
		LIR	335..340		0/4 (0%)
		Interaction with KEAP1. /evidence=ECO:0000250|UniProtKB:Q64337	346..351		1/4 (25%)
		UBA_5	378..439	CDD:318725	13/59 (22%)