Protein Alignment mib2 and mib1

DIOPT Version :10

• Sequence 1: NP_609933.1
• Gene: mib2 / 35170
• FlyBaseID: FBgn0086442
• Length: 1049
• Species: Drosophila melanogaster
• Sequence 2: NP_001123407.1
• Gene: mib1 / 100170182
• XenbaseID: XB-GENE-1001395
• Length: 1010
• Species: Xenopus tropicalis
• Alignment Length: 1096
• Identity: 382/1096 - (34%)
• Similarity: 548/1096 - (50%)
• Gaps: 158/1096 - (14%)

  Fly     8 GIRVVRGPNWIWSNQDDGEGHVGTVCEIGRCGSTHSPENTVVVNWDSGHRTNYRVGYQNQYDLII 72
            |.||.|||:|.|..||.||||||||      .|..|||..||| ||:|...|||.  ...|||.|
 Frog    15 GARVARGPDWKWGKQDGGEGHVGTV------RSFESPEEVVVV-WDNGTAANYRC--SGAYDLRI 70

  Fly    73 VDNAQVGVRHSNVVCDGCSKAGIAGIVFKCAQCPNYHLCAYCYAEDLHDIEHPFIRYTTPNSLGV 137
            :|:|..|::|...:||.|.:..|.||.:|||:|.||.||..||..|.|.:.|.|.|.|||.|..|
 Frog    71 LDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERV 135

  Fly   138 RLPMRKGAKRIQLRGIFVGSKVVRGPDWEWNEQDGGEGRTGRVMEIRGWDNESCRSVANVAWVTG 202
            .|..|:.:|:|..||||.|::||||.||:|.:||||.||.|:|.||:.|...|..|.|.|.|..|
 Frog   136 LLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNG 200

  Fly   203 STNVYRLGHKGNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDA 267
            :.|:||:|.:|..|||.:....||.:|:||.||||:....:....::     :||.|.:.||::.
 Frog   201 AKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLL-----IGDLVNIDLELEI 260

  Fly   268 LMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVK--VV------- 323
            :..||.|||||...|.|.|:..|||..|.:..||.|||.: .|||||:||.|.|  ||
 Frog   261 VQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPS-GNRWTFNPAVLTKANVVRSGDAAQ 324

  Fly   324 -------SFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDG 381
                   .|:|||||.|..|..:::.||:|||||.|.|...|||:.:|.::|||.||:::..  |
 Frog   325 GAEGGTSPFQVGDLVQICYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDNDLKVEVC--G 387

  Fly   382 FEWTLNPKCVKLERSPLATAAERSNSMMDLSHRRADHVMTPLSGLSGS-SVADKLVREAAQGHLD 445
            ..||.||..|       :..|...:::.:.:..|...::..|.....| .:.::||:.||.|.:.
 Frog   388 TSWTYNPAAV-------SRVASVGSAISNATGERLSQLLKKLFETQESGDLNEELVKAAANGDVA 445

  Fly   446 FVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQP 510
            .|...|......|:....|...:|.||..|:||::..|:....:|.|.||:||.|:|:||||::.
 Frog   446 KVEDLLKRQDVDVNGQCAGHTAMQAASQNGHVDILKLLLKHSVDVEAEDKDGDRAVHHAAFGDEG 510

  Fly   511 ATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKEN 575
            ..:.||.:.||::|..|....:.|||..:|.....|::||....:.::|||.|||.|||||.|:.
 Frog   511 TVVEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKKLLDFGCHPSLQDSEGDTPLHDAISKKR 575

  Fly   576 TEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSR----QLVNVRKDDGFAALH 636
            .:::.:|..| ..|.|:.||.|||.||||||:||..|.|  :|||:    .:|:.:||||:.|||
 Frog   576 DDILAVLLEA-GADVTITNNNGFNALHHAALRGNPSAMR--VLLSKLPRPWIVDEKKDDGYTALH 637

  Fly   637 LAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNA 701
            |||||.|.:|.|.||.:|.|.|||:|..|||...|||.:.|..::..|||....::.:|:|||..
 Frog   638 LAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDIQDKDGDTP 702

  Fly   702 MH-------LCVIKK-SNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCR 758
            :|       |..::: .::|...:......|..:...:.|  .:...|.:...||..:|:..|..
 Frog   703 LHEALRHHTLSQLRQLQDMQDVGKVDTTWEPSKNTLIMGL--GTQGAEKKSAASIACFLAANGAD 765

  Fly   759 VELNNANA-SIFEWITDRHIRQLIFGQQGEAESLPRNLQALEVSAGSADGEESSASAGAESNGAG 822
            :.:.|... |..:...|                 |...:||    .....|:||...|:.|
 Frog   766 LTIRNKKGQSPLDLCPD-----------------PNLCKAL----AKCHKEKSSGQVGSHS---- 805

  Fly   823 PGSVAAPPPPQRQTFELMPKPNDI-------PAIGVGASPSTPSASPGVKKLNSDATPQNVSPQV 880
            |..::    ...:|.|.....:|:       |.   |...:....||.|||.      .....||
 Frog   806 PSMIS----NDSETLEECMVCSDLKRDTLFGPC---GHIATCSLCSPRVKKC------LICKEQV 857

  Fly   881 APRKKAPKPPVTTSSSSTALEAGAAGPSTSPVIVPGPHECIVCNEILPMVRFEPCQHQIACEECG 945
            ..|.|.                               .||:||::....|.|:||.|..|||.|.
 Frog   858 QSRTKI-------------------------------EECVVCSDKKAAVLFQPCGHMCACENCA 891

  Fly   946 IRMKKCLRCAVAIERRL-----------------TVSGRVVALPTSTSSPSDPTRLPSGDLLRYL 993
            ..||||::|...:|||:                 ...|.:.||    ....|.|.: :.|:.: |
 Frog   892 SLMKKCVQCRAVVERRVPFVMCCSGKGTEDVTDDIAGGNIQAL----QKDKDNTNV-NADVQK-L 950

  Fly   994 ENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY 1049
            :.::.:.:|...|.:|::|.:::.|:||||.|..|.:.:..|.:|||.|.::|.||
 Frog   951 QQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY 1006

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mib2	NP_609933.1	MIB_HERC2	8..74	CDD:461991	35/65 (54%)
		ZZ_Mind_bomb	85..129	CDD:239079	20/43 (47%)
		MIB_HERC2	156..220	CDD:461991	33/63 (52%)
		SH3_15	253..319	CDD:465720	31/65 (48%)
		SH3_15	325..391	CDD:465720	31/65 (48%)
		ANKYR	398..664	CDD:440430	103/270 (38%)
		ANK repeat	464..494	CDD:293786	10/29 (34%)
		ANK repeat	496..527	CDD:293786	13/30 (43%)
		ANK repeat	529..560	CDD:293786	7/30 (23%)
		ANK repeat	596..628	CDD:293786	17/35 (49%)
		ANK repeat	630..662	CDD:293786	20/31 (65%)
		Ank_5	650..705	CDD:433530	23/61 (38%)
		ANK repeat	664..695	CDD:293786	10/30 (33%)
		PRK14959	<791..>919	CDD:184923	23/134 (17%)
		RING-HC_MIB2_rpt1	918..955	CDD:438386	17/36 (47%)
		RING-HC_MIBs-like	1004..1042	CDD:438183	14/37 (38%)
mib1	NP_001123407.1	MIB_HERC2	15..72	CDD:461991	35/65 (54%)
		ZZ_Mind_bomb	83..127	CDD:239079	20/43 (47%)
		MIB_HERC2	154..218	CDD:461991	33/63 (52%)
		SH3_15	248..311	CDD:465720	31/63 (49%)
		SH3_15	333..397	CDD:465720	31/65 (48%)
		ANKYR	444..720	CDD:440430	110/278 (40%)
		ANK repeat	464..527	CDD:293786	25/62 (40%)
		ANK repeat	529..560	CDD:293786	7/30 (23%)
		ANK repeat	562..593	CDD:293786	13/31 (42%)
		ANK repeat	595..629	CDD:293786	17/35 (49%)
		ANK repeat	631..663	CDD:293786	20/31 (65%)
		ANK repeat	665..696	CDD:293786	10/30 (33%)
		RING-HC_MIB1_rpt1	817..854	CDD:438384	9/45 (20%)
		RING-HC_MIB1_rpt2	864..901	CDD:438385	17/36 (47%)
		RING-HC_MIB1_rpt3	961..1006	CDD:438387	16/44 (36%)