DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nak and Gak

DIOPT Version :9

Sequence 1:	NP_477165.1	Gene:	Nak / 35154	FlyBaseID:	FBgn0015772	Length:	1488	Species:	Drosophila melanogaster
Sequence 2:	NP_705797.1	Gene:	Gak / 231580	MGIID:	2442153	Length:	1305	Species:	Mus musculus

Alignment Length:	1579	Identity:	334/1579 - (21%)
Similarity:	531/1579 - (33%)	Gaps:	534/1579 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly    20 PNSSSSSSSNEKNSFVGKVFTVGRVTVTVEDVLAEGGFAMVFLARGNGGGSSSATKYALKRMYVN 84
            |.|...::..:::.|||:...:|.:.:.|..||||||||.|:.|:..|.|    .:|||||:..|
Mouse    14 PGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSG----REYALKRLLSN 74

  Fly    85 NEHDLNVAKREIQIASNLSGHKNIIGYVDSSI---TPTGNGVCEVLLLMPYCKHHMLAMMNARLH 146
            .|.......:|:.....||||.||:.:..::.   ..:..|..|.|||...||..::..:.   .
Mouse    75 EEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLK---R 136

  Fly   147 VGFTEP----EVLNIFCDIAEAVSRLHYCQTPIIHRDLKVENILQTDAGNFVLCDFGSATAKTLN 207
            |....|    .:|.||.....||..:|..:.|||||||||||:|.::.|...||||||||..:..
Mouse   137 VECKGPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHY 201

  Fly   208 P------QQHGVTVVQEEIQKYTTLSYRAPEMIDLYGGKSITTKADIWALGCMLYKLCFFSLPFG 266
            |      |:.  .:|:|||.:.||..||.||::|||....|..|.|||||||:||.|||...||.
Mouse   202 PDYSWSAQKR--AMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFE 264

  Fly   267 E-STLAIQNGQFSIP-DSSKYSKGMHQLIKYMLEPDMEKRPNIWQVCEVAFRLAGKDNPVQNLHK 329
            : :.|.|.||::||| :.::|:. .|.||:.||:.:.|:|.:|.:|..
Mouse   265 DGAKLRIVNGKYSIPVNDTRYTV-FHDLIRAMLKVNPEERLSIAEVVR----------------- 311

  Fly   330 TPIPNFDLLVVPPFESEAKRISAAASKAAKAQNVSVVEA----GTSVAPRSRPKGSSSVHGGNPL 390
                            :.:.|:||.:...||....::|.    |.|...|::|....:|:....|
Mouse   312 ----------------QLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVL 360

  Fly   391 GLGLPPSPSPRQNITSPQPQP--------QPVVEQFQANFPAMPPAVPPPPQAVTPAAPAASKLF 447
            .|             :...||        :...|:...|.                 ...:||:.
Mouse   361 AL-------------AEYDQPYGGFLDILRGGTERLFTNL-----------------KDTSSKVI 395

  Fly   448 ES-SGYPDPFNEPAAAVIPTEFLPAAEEPNKEEVPQDLNVIAGTPTKSMLTPPKPSGGGGHRRNV 511
            :| :.|                           ...||::...|...::::.|........:.|:
Mouse   396 QSVANY---------------------------AKGDLDISYITSRIAVMSFPAEGVESAIKNNI 433

  Fly   512 SDTSAFNKTFANETSQFLAPFNNNLAAMGDGQQTLASAASNPGIYASSTANSAAVSISELMKPGQ 576
            .|...|                  |.|...|...:.:.  :|.||.:|..::........::...
Mouse   434 EDVRMF------------------LDAKHPGHYAVYNL--SPRIYRASKFHNRVTECGWAVRRAP 478

  Fly   577 -----TAVEKRVEAWNPFEEEQPFSQMTEDHIFEAEFDKIRQRGSQGSITAKSASTTST---LTP 633
                 ..:.:.:.||           :.|||         |.......:..::||..:.   |..
Mouse   479 HLHSLYTLCRSMHAW-----------LREDH---------RNVCVVHCMDGRAASAVAVCAFLCF 523

  Fly   634 TEQIPSAVVAPVTAAPGAVPP---PPIAAAVSPYPSQVSEDPFGSAPFSLPPGLREKASSLRKTG 695
            .....:|..|....:....||   |.....:......|:|:|.  .|.|.|..::..
Mouse   524 CRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPI--TPHSKPMLVKSV-------- 578

  Fly   696 AKFIGVSSGGTGGGVATPIGLVAGGANTATSSNSSKWMLSPTLEV-SSEEKTSLIS----KLK-- 753
                          |.||:.|.:...|..          .|..|| ..||:.:..|    ::|
Mouse   579 --------------VMTPVPLFSKQRNGC----------RPFCEVYVGEERVTTTSQEYDRMKEF 619

  Fly   754 --TDSECGIPGAVGGAGEGVSGYVKLPVEDRNKYEKLRSKDQPTSDDSDSEASEFFQQDSDEGGG 816
              .|.:..||     .|..|.|.|.:.:                           :...:..||
Mouse   620 KIEDGKAVIP-----LGVTVQGDVLIII---------------------------YHARATLGG- 651

  Fly   817 GIFRQMQMVTSNLPETIHKIQQVAYHKVDKTAHLPIVKKLRDKTKKPTTAAHQAAQQLNLMAAAV 881
                ::|...:::     |:.|:.:|    |..:|         :..||
Mouse   652 ----RLQAKMASM-----KMFQIQFH----TGFVP---------RNATT---------------- 678

  Fly   882 AAQAEEAAKAAESDGDSIGSASDLRAEDDDLFDEN------PRQTQAKQRRPLP-TEMDGISESV 939
                   .|.|:.|.|    |.|::.:..|||..|      ||...:::..|.. |.:.|::..:
Mouse   679 -------VKFAKYDLD----ACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWENTSLRGLNPKI 732

  Fly   940 ----------------------KTCSSSAYHAEC---ESVTTHEDDVRS---------------- 963
                                  :..|::.|.||.   |:..|..|..:|
Mouse   733 LFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQE 797

  Fly   964 ----RVVVKVRMRKKDRALVTATGD---------PSAPSEELSPTSSDLLHKFGDRPLLL----D 1011
                ...:.....|:.::::.|.||         .|.||||..|.:.:      |.|.|.    .
Mouse   798 EKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGE------DTPRLAAGTKQ 856

  Fly  1012 DELDYGSGESKSSTESPVAPILPTNPDVQESEEDVFALAPFKLPVGVPLKRKTKPKAKPKPSEPE 1076
            .:|.:..| ..::.:.||.|....:.....||.|:...||.: ..|||...........:||:
Mouse   857 QDLIFDVG-MLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQ-ACGVPSSNTDLLSCLLEPSD-- 917

  Fly  1077 MWTSTPVKSAAVGDPSTNFASFPAQL--TPTNNVGNSAQLDEFNEPIFNPFQENAQNTQSGETNQ 1139
                    :|.||.|..........|  :|.:.:|....|........:||.:...::.| :|..
Mouse   918 --------AAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNS-DTQP 973

  Fly  1140 C---DLFGLEPFPQVIR--TIEQNPQHHQIHQFPSHNNNNNVIKVPASVSVA------LPASVPV 1193
            |   ||||     :.:.  ::..:......|..|           |.|.|.|      |||. |.
Mouse   974 CSKPDLFG-----EFLNSDSVASSTAFPSTHSAP-----------PPSCSTAFLHLGDLPAE-PS 1021

  Fly  1194 SVSVNSSAPQL-----------------VTINHSIIINKT--PEPPMNYNNNNNNQTAKSSGTSS 1239
            .|..:||.|.|                 :.:....:.:..  |.||    ..|.:|| ||.
Mouse  1022 KVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPP----GPNPSQT-KSQ---- 1077

  Fly  1240 VYVNVPSFSSMSTSSCSINQPQSSPAAVIP----VQAPLPVP---VPAPVSVPAVP--------V 1289
               |:..|:.:|..|.|:   |..||. :|    |.||.|..   .|...:.|..|        .
Mouse  1078 ---NLDPFADLSDLSSSL---QGLPAG-LPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAK 1135

  Fly  1290 PIPAVVTHLRPLLPIAD---------NAYVQ---ISQDRCSDFTPD------DEEEPVVL----- 1331
            |.|.....||....:..         .::.|   :|::...|..|:      |::.|..:
Mouse  1136 PAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRK 1200

  Fly  1332 ----RDAD--------------------------VVADG---------IGIPTPAKPKKEKSNLA 1357
                ||.|                          |:.||         ..:.||.:.||:.....
Mouse  1201 QELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAV 1265

  Fly  1358 VRVLPGKLTQKVKGHSYKK 1376
            :.|.|.|.|    |..|::
Mouse  1266 LVVHPDKAT----GQPYEQ 1280

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nak	NP_477165.1	STKc_NAK_like	42..319	CDD:270939	112/291 (38%)
Nak	NP_477165.1	S_TKc	47..312	CDD:214567	111/279 (40%)
Gak	NP_705797.1	STKc_GAK	39..320	CDD:270938	113/323 (35%)
		S_TKc	41..311	CDD:214567	111/279 (40%)
		PTEN_C2	568..706	CDD:287393	42/251 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	707..732		5/24 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	747..854		21/112 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1037..1139		25/117 (21%)
		DnaJ	<1253..1298	CDD:99751	10/32 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1989
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D296562at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.820