DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ham and PRDM16

DIOPT Version :9

Sequence 1:	NP_724130.4	Gene:	ham / 35135	FlyBaseID:	FBgn0045852	Length:	1108	Species:	Drosophila melanogaster
Sequence 2:	NP_071397.3	Gene:	PRDM16 / 63976	HGNCID:	14000	Length:	1276	Species:	Homo sapiens

Alignment Length:	1234	Identity:	358/1234 - (29%)
Similarity:	496/1234 - (40%)	Gaps:	354/1234 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    69 PRRDHLDIQDQELLHHPNHFHQQNQRQQP----PPPTLPLSVESSSAAAASVDGMDAFFKDRAQA 129
            |.||       .|..|......::....|    ||...|.|.:               |..:..:
Human    24 PNRD-------LLASHSAEDEAEDSAMSPIPVGPPSPFPTSED---------------FTPKEGS 66

  Fly   130 EHILQEWVRRREPV-CELDIRDSG------GVYAKTPLQRGTRYGPFPMKLSHQPNDPQLAWKAH 187
            .:....::....|: .:.::|:|.      ||:||..::.|.|.||..:.       |:.|.|..
Human    67 PYEAPVYIPEDIPIPADFELRESSIPGAGLGVWAKRKMEAGERLGPCVVV-------PRAAAKET 124

  Fly   188 SRHYNGWLEPTEDV------------------------------STWLKKIRSVQDDCIGEANLQ 222
            .   .||.:...||                              .:|||.|| |...| .:.||.
Human   125 D---FGWEQILTDVEVSPQEGCITKISEDLGSEKFCVDANQAGAGSWLKYIR-VACSC-DDQNLT 184

  Fly   223 SYINAGYLWYETNRYVNAGSEMVV-------------DGRPKSPV----------QLNEDFMNGG 264
            ....:..::|:..:.:..|.|::|             .|..:.|.          |...|.....
Human   185 MCQISEQIYYKVIKDIEPGEELLVHVKEGVYPLGTVPPGLDEEPTFRCDECDELFQSKLDLRRHK 249

  Fly   265 KVLAAAAAAAAAVVAASSSGAKSGGGGSSAPSDDRSDRDNGSLYSGDEFSKDKKNSSLIREGDID 329
            |....:..||.....|.....:..||||....:
Human   250 KYTCGSVGAALYEGLAEELKPEGLGGGSGQAHE-------------------------------- 282

  Fly   330 FTDDENGFDIRCEVCDKVYPDLDLLDDHLIGAHHFKQDEFPCKQCALRFCHRPLLIKHEAISHNN 394
                       |:.|::::|:...|:.|::  .|.::.|:.|.||...|..:..||:|: :||::
Human   283 -----------CKDCERMFPNKYSLEQHMV--IHTEEREYKCDQCPKAFNWKSNLIRHQ-MSHDS 333

  Fly   395 IRKYSCENCSKVFCDPSNLQRHIRAYHVGARCHPCPECGKTFGTSSGLKQHQHIHSSVKPFACEV 459
            .:::.||||.|||.||||||||||:.|||||.|.||:|||||.||||||||:||||:||||.|||
Human   334 GKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEV 398

  Fly   460 CSKAYTQFSNLCRHKRMHATCRMQIKCDKCNQSFSTLTSLTKHKKFCDSTGPGPYRNQHVNRHHQ 524
            |.|:|||||||||||||||.||.||||..|.|.|||.:||.||::||:            .::|.
Human   399 CHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCE------------GKNHY 451

  Fly   525 HPHQHPLPHQPHLAATATSTCPAPPRESSESSSSAAAAAVA---AMSTPPNP-FLMFRTAPSFFP 585
            .|.....|..|        ..|:|..:.::.|.|...|::.   ...:.|:| .|.|.|||..||
Human   452 TPGGIFAPGLP--------LTPSPMMDKAKPSPSLNHASLGFNEYFPSRPHPGSLPFSTAPPTFP 508

  Fly   586 G--------FPP--YGFPPFLP-----QNPLHPTNIPMFFSKNPMDLGCGGPEITSPVSAFDQKL 635
            .        |||  |..||.||     ::||:.|.    .:|.|..||.....:.|.||...|..
Human   509 ALTPGFPGIFPPSLYPRPPLLPPTSLLKSPLNHTQ----DAKLPSPLGNPALPLVSAVSNSSQGT 569

  Fly   636 PFGFLKGENSESQAYDKVTEKELVFKAEEKLKKEPLVQAFEGEEDESRSSLDIKGKLEDTRNDSK 700
            .......|..||:..|...||       .|.:...:....:.|:..:.:..|:     ||...:.
Human   570 TAAAGPEEKFESRLEDSCVEK-------LKTRSSDMSDGSDFEDVNTTTGTDL-----DTTTGTG 622

  Fly   701 SEEQDDMKQEPERVSTPDQ---QQAEDDRKSIDIMSTP-------------------PPAD---- 739
            |:...|:..:|::    |:   :.||...|....::.|                   ||.|
Human   623 SDLDSDVDSDPDK----DKGKGKSAEGQPKFGGGLAPPGAPNSVAEVPVFYSQHSFFPPPDEQLL 683

  Fly   740 --TPSGGDGPLDLSICRKRSAGSFFTAPAEDNL------------MLHRFMPRLHEF-------- 782
              |.:.||....::...::..|..|....|..|            .|..|...|:.|
Human   684 TATGAAGDSIKAIASIAEKYFGPGFMGMQEKKLGSLPYHSAFPFQFLPNFPHSLYPFTDRALAHN 748

  Fly   783 ---EAERGQPL-KMRKSHSSAES----STSQKSHKGSSPTPTPTASPGLTPSPSPPTSAGGELSS 839
               :||...|. .::....|||.    :|..|..|...|.|...::|..........|.|....:
Human   749 LLVKAEPKSPRDALKVGGPSAECPFDLTTKPKDVKPILPMPKGPSAPASGEEQPLDLSIGSRARA 813

  Fly   840 TSEGGAVPTMAAAAFAADHSALASGPTLPQT-------HPTFH----------PL---------- 877
            :..||.........:.  ...|.:|..|||.       .|..|          |:
Human   814 SQNGGGREPRKNHVYG--ERKLGAGEGLPQVCPARMPQQPPLHYAKPSPFFMDPIYSRVEKRKVT 876

  Fly   878 ----LLEEIYRSGFPFLCQPGGRRGIEALLAGAASAAAPK-------RPLP--PVKF-----SAG 924
                .|:|.|....|.|..| ....||.:.....|.||.|       :|||  |..|     :..
Human   877 DPVGALKEKYLRPSPLLFHP-QMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLS 940

  Fly   925 SVVGLKTKDRYSCKFCGKVFPRSANLTRHLRTHTGEQPYPCKYCDRAFSISSNLQRHVRNIHNKE 989
            ..:..|.|:||:|::|||:||||||||||||||||||||.||||||:||||||||||||||||||
Human   941 DPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKE 1005

  Fly   990 RPFRCELCDRSFGQQTNLDRHVKKHESEG--------------NNFRDSPSSSG-----IAEREE 1035
            :||:|.||:|.||||||||||:||||.|.              .....||:|..     :.|:|:
Human  1006 KPFKCHLCNRCFGQQTNLDRHLKKHEHENAPVSQHPGVLTNHLGTSASSPTSESDNHALLDEKED 1070

  Fly  1036 -YFDDIRKF-----MNRVYT-----------------PNSLAGNEGDTEEYPNSDDQSVNLEKD 1076
             ||.:||.|     ||:..|                 |...:..:.|.||..:.|     ||:|
Human  1071 SYFSEIRNFIANSEMNQASTRTEKRADMQIVDGSAQCPGLASEKQEDVEEEDDDD-----LEED 1129

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ham	NP_724130.4	PR-SET_ZFPM	131..253	CDD:380978	32/171 (19%)
		C2H2 Zn finger	341..363	CDD:275368	5/21 (24%)
		C2H2 Zn finger	371..392	CDD:275368	7/20 (35%)
		SFP1	<391..479	CDD:227516	67/87 (77%)
		C2H2 Zn finger	400..421	CDD:275368	17/20 (85%)
		SUF4-like	403..>445	CDD:411020	33/41 (80%)
		C2H2 Zn finger	403..421	CDD:411020	14/17 (82%)
		C2H2 Zn finger	429..449	CDD:275368	16/19 (84%)
		C2H2 Zn finger	457..477	CDD:275368	17/19 (89%)
		C2H2 Zn finger	486..504	CDD:275368	10/17 (59%)
		PTZ00121	<641..>728	CDD:173412	17/89 (19%)
		COG5048	935..>1028	CDD:227381	72/106 (68%)
		zf-C2H2	935..957	CDD:395048	17/21 (81%)
		C2H2 Zn finger	937..957	CDD:275368	16/19 (84%)
		zf-H2C2_2	949..973	CDD:404364	21/23 (91%)
		C2H2 Zn finger	965..986	CDD:275368	19/20 (95%)
		C2H2 Zn finger	994..1014	CDD:275368	15/19 (79%)
PRDM16	NP_071397.3	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..68		11/65 (17%)
		SET	84..209	CDD:214614	29/136 (21%)
		C2H2 Zn finger	283..303	CDD:275368	5/21 (24%)
		zf-H2C2_2	295..319	CDD:290200	7/25 (28%)
		C2H2 Zn finger	311..331	CDD:275368	7/20 (35%)
		zf-C2H2	337..358	CDD:278523	16/20 (80%)
		C2H2 Zn finger	339..357	CDD:275368	15/17 (88%)
		zf-C2H2	366..388	CDD:278523	17/21 (81%)
		C2H2 Zn finger	368..388	CDD:275368	16/19 (84%)
		zf-C2H2_8	369..450	CDD:292531	59/92 (64%)
		zf-C2H2	394..416	CDD:278523	18/21 (86%)
		C2H2 Zn finger	396..416	CDD:275368	17/19 (89%)
		C2H2 Zn finger	425..443	CDD:275368	10/17 (59%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	533..657		29/143 (20%)
		Interaction with CTBP1, CTBP2 and ZNF516. /evidence=ECO:0000250\|UniProtKB:A2A935	679..1038		126/361 (35%)
		Mediates interaction with SKI and regulation of TGF-beta signaling. /evidence=ECO:0000269\|PubMed:19049980	739..1276		136/399 (34%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	772..804		6/31 (19%)
		COG5048	931..>1083	CDD:227381	84/151 (56%)
		zf-C2H2	951..973	CDD:278523	17/21 (81%)
		C2H2 Zn finger	953..973	CDD:275368	16/19 (84%)
		zf-H2C2_2	965..989	CDD:290200	21/23 (91%)
		zf-C2H2	979..1002	CDD:278523	20/22 (91%)
		C2H2 Zn finger	981..1002	CDD:275368	19/20 (95%)
		zf-C2H2	1008..1030	CDD:278523	16/21 (76%)
		C2H2 Zn finger	1010..1030	CDD:275368	15/19 (79%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1033..1065		4/31 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1105..1163		8/30 (27%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	53	1.000	Domainoid score	I11359
eggNOG	1	0.900	-	-		E33208_3BAJ6
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	409	1.000	Inparanoid score	I1880
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D254483at33208
OrthoFinder	1	1.000	-	-		FOG0002720
OrthoInspector	1	1.000	-	-		otm41391
orthoMCL	1	0.900	-	-		OOG6_105951
Panther	1	1.100	-	-	LDO	PTHR24393
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4598
SonicParanoid	1	1.000	-	-		X2993
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.860