DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment kon and Cspg4

DIOPT Version :9

Sequence 1:	NP_001260539.1	Gene:	kon / 35104	FlyBaseID:	FBgn0032683	Length:	2381	Species:	Drosophila melanogaster
Sequence 2:	NP_112284.2	Gene:	Cspg4 / 81651	RGDID:	619942	Length:	2326	Species:	Rattus norvegicus

Alignment Length:	2559	Identity:	599/2559 - (23%)
Similarity:	1037/2559 - (40%)	Gaps:	437/2559 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    14 LAWLAILQLQTVAAMKVSLFGDGYVSMPLQEAKMSTNIRVKFRTRQENAFLFLAAGRTDYCLLRL 78
            ||.:..|.|...:....|.||:.::.:|:..|....::.::|.|.|..|.|.||||:||:.||:|
  Rat    14 LALILTLALFVRSTAPASFFGENHLEVPVPSALTRVDLLLQFSTSQPEALLLLAAGQTDHLLLQL 78

  Fly    79 ESGLISFSYKIERDVVQLRSPKKQKLNDLEWHDVAVQRFENNITLQVDGYIMRKQLPGDLAALNI 143
            :||.:.....:.::.:.|::|....|:|...|.|.:....:...|.|||.:....|....:.|.:
  Rat    79 QSGHLQVRLALGQNELSLQTPADTVLSDSTTHTVVLTVSNSWAVLSVDGVLNTSALIPKASHLKV 143

  Fly   144 HFGTFLGGVGDFTAEFLDDVI-GFRGCISDVFYNNINIIKRAKDRTSHTTSTGVAWTCSTEFEGS 207
            .:|.|:|..|.....:|..:. ..|||:.....|..|:::        ..:..|...|:.||  |
  Rat   144 PYGLFVGSSGSLDLPYLKGISRPLRGCLHSAILNGRNLLR--------PLTPDVHEGCAEEF--S 198

  Fly   208 IQDSISFMRNDSYSLMMKESYAMGE--TLSLQFRTMASAGVIFFNGG---YDFILLEIEDQHLKV 267
            ..|.:....:..:||....:::..|  ||.....|.:....:.|..|   .:||.::|.:.||:.
  Rat   199 AGDEVGLGFSGPHSLAAFPAWSTREEGTLEFTLTTRSQQAPLAFQAGDKRGNFIYVDIFEGHLRA 263

  Fly   268 TFNKAGSLVQFMTNEHISDGKWHRVFLRYNAAIAELSLDDATSGYRGTHANETKASINLEKSVFF 332
            ...|....:....:..::||:.|.|.:..:....|:|:|...:   .|......:.:....|:..
  Rat   264 VVEKGQGTMLLRNSVPVADGQPHEVSVHIDVHRLEISVDQYPT---RTFNRGVLSYLEPRGSLLL 325

  Fly   333 GGVQEEMRRRLISKGLRIN----EISFKGCMRDILVNDLPLGFAEMTISRSLALNCLWKYPCVEY 393
            ||:..|..|.|....|.:.    .||..||:.|..||...||..:..::|.:|..|..:
  Rat   326 GGLDTEASRHLQEHRLGLTPGAANISLVGCIEDFSVNGRRLGLRDAWLTRDMAAGCRPE------ 384

  Fly   394 NPCLKSGICSQHGVDGFICYCDQSYCIKADFQGPFKIFTETSPE--------------------- 437
                                 :..|  :.:..|||:.|:..:||
  Rat   385 ---------------------EDEY--EEEVYGPFEAFSTLAPEAWPVMDLPEPCVPEPGLPAVF 426

  Fly   438 ---LELLYVSPMQLLEGGTAFLSPHFIDIILDLRRYPSLNEQSIIFHVVHQPKYGQLLQYSAEKA 499
               .:||.:||:.:.|||||:|....:...|||.. ..|.:..::|.|....::|:|       .
  Rat   427 ANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLTE-AELRKSQVLFSVSQGARHGEL-------E 483

  Fly   500 IFVP----CRTFNLVDLATDKLKYVHNGQENFNDHATLDMQIFGDVHKIPENILGKHRFLLHANI 560
            :.:|    .:.|.|:|:...|.::||:|.|:.:|...|::.:.... .:|..:.....::|...:
  Rat   484 LDIPGAQTRKMFTLLDVVNRKARFVHDGSEDTSDQLMLEVSVTSRA-PVPSCLRRGQIYILPIQV 547

  Fly   561 TPINDPPQLRLHSHKILRVIEGIERVLDVDLFNIDDPDSEPGNLIYTIL------PTQSPQETFG 619
            .|:||||::......::.::|..::.|..::|...||||....|...:|      |.:...:.
  Rat   548 NPVNDPPRIVFPHGSLMVILEHTQKPLGPEIFQAYDPDSACEGLTIQLLGVSASVPVEHRDQP-- 610

  Fly   620 CFIVGGATTSAFSQAEVNVGKVSYLYNSTTAESFSYELQLQVSDGIETSETVYLP-VSVHPLELR 683
                 |...:.||..::..|.:.|::....|:    :|..:||||::.|....|. |:|.| .::
  Rat   611 -----GEPVTEFSCRDLEAGNIVYVHRGGPAQ----DLTFRVSDGMQASGPATLKVVAVRP-AIQ 665

  Fly   684 LVNNTGLIMIHKSSLPISTANLSIGTNAVDDHIDIRYDIVKAPQHGVLQRLRQIDGSWVNVDW-- 746
            :::||||.:...|:..|..||||:.||||...:.:.:.:....|.|.||  :|..|.....:|
  Rat   666 ILHNTGLRLAQGSAAAILPANLSVETNAVGQDVSVLFRVTGTLQFGELQ--KQGAGGVEGTEWWD 728

  Fly   747 ---FSDSQLLLGHIRYL------HSSDFPWQDEFKFIASFGFVTTQTFDFRITFTRLRITSTRPS 802
               |....:..|.:|||      |:.|  ..::.......|..|.....|.:|..|..:...|..
  Rat   729 TLAFHQRDVEQGRVRYLSTDPQHHTQD--TVEDLTLEVQVGQETLSNLSFPVTIQRATVWMLRLE 791

  Fly   803 QISINGSREILLTSDVLS---YETTPIGSFARSVIYKITKPTRYGGIYVEGSRKAAKKLDSFTQQ 864
            .:......:..|||..|.   .|....|.:.....|::.:..|.|.:.::|:|.:..:  ||:|.
  Rat   792 PLHTQNPHQETLTSAHLEASLEEEGEGGPYPHIFHYELVQAPRRGNLLLQGTRLSDGQ--SFSQS 854

  Fly   865 DIEKRRIRYQTHHTSYSSFSDHLEFVVSVAECDDVAGVLEINYRPPDELINKLGYQNHEP----- 924
            |::..|:.|:....:..:..|...|.|:...          ::.|.......:|...:.|
  Rat   855 DLQAGRVTYRATTRTSEAAEDSFRFRVTSPP----------HFSPLYTFPIHIGGDPNAPVLTNV 909

  Fly   925 -LQVQEGERALITKNHFGIRFNIYESLQFQVSLSPEHGVICKYDEQTGLTTPVEMFTLEQLFRND 988
             |.|.||...:::.:|..::.....|..::|...|.||.:...|.: |..|||..||.|.|....
  Rat   910 LLMVPEGGEGVLSADHLFVKSLNSASYLYEVMEQPHHGSLAWRDPK-GRATPVTSFTNEDLLHGR 973

  Fly   989 IYYCHDDTESTRDTFELLIL-----SGDETDLQFVSNMEVHIKLVNDNEPYRTAIERVFHVVRNG 1048
            :.|.|||:|:..|....:..     |||....:......|.|:.|||:.|.:| |.|||||.|.|
  Rat   974 LVYQHDDSETIEDDIPFVATRQGEGSGDMAWEEVRGVFRVAIQPVNDHAPVQT-ISRVFHVARGG 1037

  Fly  1049 IRTLNPTVLQYLDADVNTNHTDIHYIHVSSTNG---AFYKSGHYIDSFTQDDIANRRIMFQHTGA 1110
            .|.|....:.:.|||...:...:.........|   |..:....|..|||:|:..::::|.|:||
  Rat  1038 QRLLTTDDVAFSDADSGFSDAQLVLTRKDLLFGSIVAMEEPTRPIYRFTQEDLRKKQVLFVHSGA 1102

  Fly  1111 DSGTASFIVTDREHEVNGLLEIRASDPFVSMMATNASIVQEGKFVVLKNKDFILETNLDMKL-DE 1174
            |.|.....|:|.:|:...:||::||:|::.:..:::.:|.:|....:......|:||||::. :|
  Rat  1103 DHGWLQLQVSDGQHQATAMLEVQASEPYLHVANSSSLVVPQGGQGTIDTAVLHLDTNLDIRSGNE 1167

  Fly  1175 IYYEVIKPPSYGILMYLSRANEGENGTTIYKATNYTSLSNFTHLDIERERLVY-WNTEIASMDKV 1238
            ::|.|...|.:|.|:     .:|:            |:::|:..|:....::| .|..::..|.:
  Rat  1168 VHYHVTAGPHWGQLL-----RDGQ------------SVTSFSQRDLLDGAILYSHNGSLSPQDTL 1215

  Fly  1239 RYRVHIKNITAEGEVMFRIYPSAYWEPLQVKQNHTLYVEESTSVLISRDVLEVVHPNISPGDITF 1303
            ...|....:.....:...|.......|||:.|:..:||.:..:..|.||.||||...:.|.||.|
  Rat  1216 ALSVAAGPVHTSTVLQVTIALEGPLAPLQLVQHKRIYVFQGEAAEIRRDQLEVVQEAVLPADIMF 1280

  Fly  1304 LVTSSPMHGYLEMQS--MSFD-----DEYNCKVFDQSSVNTEKMFYIQAGVNQSTDYFVFDVTNG 1361
            .:.|.|..|||.|.|  .|.|     |...|  |.|.::|:.::.|:.:.....:|.|..||.:|
  Rat  1281 SLRSPPNAGYLVMVSHGASADGPPSLDPVQC--FSQEAINSGRVLYLHSRPGAWSDSFSLDVASG 1343

  Fly  1362 I-TWLRQLMIKIVIIPEKLYMHSNIISVVEGKTVQLNPTDIQ---PYSEYYRGKILEYIVTITPS 1422
            : ..|..:.:::.::|..:.:.....||.||.|..|.|..||   ||.....|.:|:  |...|.
  Rat  1344 LGDPLEGISVELEVLPTVIPLDVQNFSVPEGGTRTLAPPLIQITGPYFPTLPGLVLQ--VLEPPQ 1406

  Fly  1423 SGHV----LAGNSKVKRFTQKQLEQGSIQYVHNGSENATDSITLVAMA--RNKESVPFELEFAVV 1481
            .|.:    ...:..:..|:.:::|:..|:|||:|||..||...|:|.|  .:::|.|......::
  Rat  1407 HGALQKEDRPQDGTLSTFSWREVEEQLIRYVHDGSETQTDGFILLANASEMDRQSQPMAFTITIL 1471

  Fly  1482 QVNDEEPMMVTNTGLQVWNGGRYVIKNTDLLAQDYDTPPENLTFVVNHIYGGYLARKSAPHQKIE 1546
            .|||:.|::.||||||:|.|....|....|...|.|:.||:|.:.:.....|.:|.:.||..:..
  Rat  1472 PVNDQPPVITTNTGLQIWEGAIVPIPPEALRGIDSDSGPEDLVYTIEQPSNGRIALRVAPDAEAH 1536

  Fly  1547 HFTQAQINLEEIYFMHDSNSRRNELSFVVTDGLFNTTTQMLNIEI-KPIEILAEHNENLHVFPLT 1610
            .|||||::...:.|.| ..:......|.::||:..:......:.. |.:.:..|.:..|.|.|.:
  Rat  1537 RFTQAQLDSGLVLFSH-RGALEGGFHFDLSDGVHTSPGHFFRVVAQKQVLLSLEGSRKLTVCPES 1600

  Fly  1611 KKQILRDYLHFKCS--DEEREIRYNITVPPSLGRIVNEFIDNGFTKE-VSEFTQNDVDNGHIFYE 1672
            .:.:....|....|  .:.|.:.|.:...|.|||:::  ...|..:| :..|||.:|:.|:|.||
  Rat  1601 VQPLSSQSLSASSSTGSDPRHLLYQVVRGPQLGRLLH--AQQGSAEEALVNFTQAEVNAGNILYE 1663

  Fly  1673 HTAVIMEF-RTNDSF--------------------YFDVVA-ERSDRLLNQKFNIEISVSSGGLL 1715
            |......| ..:|:.                    .||... :|..||...|          ||.
  Rat  1664 HEISSEPFWEAHDTIGLLLSSSPARDLAATLAVTVSFDAACPQRPSRLWRNK----------GLW 1718

  Fly  1716 RFLPVNKLNVDEGGSVPI---KLDFSKILEYLKTKAGINNPELFIEAIQKPTHGNIGLGHEFKHM 1777
                     |.||....|   .||.:.:|..:.......:..|| :..|.||.|.:.:..|..|.
  Rat  1719 ---------VPEGQRAKITVAALDAANLLASVPASQRGRHDVLF-QITQFPTRGQLLVSEEPLHA 1773

  Fly  1778 QRYH--PSDFFTKKVYYIHDHSDTLEDTILMSVYLTQG------------NIFLCNLTIPVSINP 1828
            :|.|  .|:....::.|.|....|.:|......:| ||            ..|:      :::..
  Rat  1774 RRPHFLQSELTAGQLVYAHGGGGTQQDGFRFRAHL-QGPTGASVAGPQTSEAFV------ITVRD 1831

  Fly  1829 INDQPFHLVTHLPQMTVVEGENRTITRNDLLTEDADTPPEEIIYDVMSGPTLGVLRKITNDGRPE 1893
            :|::|......:| :.:..|....::|..|...|.|:.|.||.|:|...|..|.|....::..|
  Rat  1832 VNERPPQPQASIP-LRITRGSRAPVSRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLAGDNTGP- 1894

  Fly  1894 DLLAFSNQFTQADINNDRIIYVHFGMPQSTTFCFTVSDGQSNPAYEIFTIKI--DSIHLQPSAMQ 1956
                 ...|||||::..|:.:|..|...:..|..::|||.|.|......:.:  .:|.:|   ::
  Rat  1895 -----VTHFTQADVDAGRLAFVANGSSVAGVFQLSMSDGASPPIPMSLAVDVLPSTIEVQ---LR 1951

  Fly  1957 APVKVQQGATTAPMRLDHIGVSTNVHMERLSYNVTNSPLFGIIVYKHQPTLRFTQQQLETSQISY 2021
            ||::|.|....:.:....:.|.::.....::|.:|..||:|.::...||...|:|.|::...:.:
  Rat  1952 APLEVPQALGRSSLSRQQLQVISDREEPDVAYRLTQGPLYGQVLVGGQPASAFSQLQVDQGDVVF 2016

  Fly  2022 MQTDLNRSNDSFQVSAYVPGTNYVAQVDVVMEVEPVIQINDIVMKEAESSGGKIKLITSLDNDNP 2086
            ..|:.:.|.|.|:|.|...|.|..|.|:|.  |:.::.:         .:||.....|:|..| |
  Rat  2017 AFTNFSSSQDHFKVLALARGVNASATVNVT--VQALLHV---------WAGGPWPQGTTLRLD-P 2069

  Fly  2087 LSLKLNKF------NPKFVITRMPSTGQIRKIIRSTGLTTDGQSDRSTN----IFSYKELRSGVV 2141
            ..|..::.      .|:|.:...|..|::.::       :.|:::..||    .|:.::|..|.:
  Rat  2070 TVLDASELANRTGSMPRFRLLEGPRYGRVVRV-------SQGRAESRTNQLVEDFTQQDLEEGRL 2127

  Fly  2142 YF-VPHESVEETEADHDSFDYQLLIKTVQPAQA------------------TVSIEYRSRVE--- 2184
            .. |.......|....|....:|....|.||.|                  .:|:...:|.|
  Rat  2128 GLEVGRPEGRSTGPTGDRLTLELQATGVPPAVALLDFATEPYHAAKFYKVTLLSVPEAARTETEK 2192

  Fly  2185 ---ETETVHLGSAGLS----------INYLAIGCAIFIVLICLLIVLLIL---------KIRKLR 2227
               .|.|...|.|..|          :.:|.......|:.:||:::||.|         |..|..
  Rat  2193 TGKSTPTGQPGQAASSPMPTVAKSGFLGFLEANMFSVIIPVCLVLLLLALILPLLFYLRKRNKTG 2257

  Fly  2228 KHKADISKDQPPALPCPPDLTSVSPQHHLHHHHGHHYASSEADSVPATGNSTPLPGFSNIPHCKI 2292
            ||...:             ||                      :.|..|.:.....|..:...:.
  Rat  2258 KHDVQV-------------LT----------------------AKPRNGLAGDTETFRKVEPGQA 2287

  Fly  2293 IPVESYKHEYPDYDPDEEETDDQCDPQQMMLPQRYNPYHMEHDAWSSSCDMANDFAGYASVPQSI 2357
            ||:.:...:.|       ....|.||:.:.                           :...|
  Rat  2288 IPLTTVPGQGP-------PPGGQPDPELLQ---------------------------FCRTP--- 2315

  Fly  2358 SGSVSSPPSAPPTNPLLRRNQYWV 2381
                         ||.||..||||
  Rat  2316 -------------NPALRNGQYWV 2326

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
kon	NP_001260539.1	LamG	30..176	CDD:238058	41/146 (28%)
		LamG	211..365	CDD:238058	34/162 (21%)
		Cadherin_3	507..664	CDD:292802	36/162 (22%)
		Cadherin_3	631..776	CDD:292802	43/156 (28%)
		Cadherin_3	747..892	CDD:292802	32/153 (21%)
		Cadherin_3	861..1005	CDD:292802	36/149 (24%)
		Cadherin_3	979..1121	CDD:292802	47/149 (32%)
		Cadherin_3	1093..1242	CDD:292802	38/150 (25%)
		Cadherin_3	<1264..1361	CDD:292802	36/103 (35%)
		Cadherin_3	1330..1464	CDD:292802	39/141 (28%)
		Cadherin_3	1436..1578	CDD:292802	47/143 (33%)
		Cadherin_3	1789..1932	CDD:292802	35/154 (23%)
		Cadherin_3	2009..2163	CDD:292802	35/164 (21%)
Cspg4	NP_112284.2	Globular or compact configuration stabilized by disulfide bonds	30..640		149/671 (22%)
		Neurite growth inhibition	30..640		149/671 (22%)
		Laminin_G_1	55..182	CDD:395008	38/126 (30%)
		LamG	204..362	CDD:238058	34/160 (21%)
		Cadherin_3	<437..524	CDD:406568	26/94 (28%)
		Cadherin_3	537..646	CDD:406568	25/119 (21%)
		CSPG 2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01201, ECO:0000269\|PubMed:12220645	554..646		20/102 (20%)
		Interaction with COL6A2	575..1044		126/498 (25%)
		Interaction with COL5A1	632..1450		225/861 (26%)
		Cadherin_3	665..744	CDD:406568	23/80 (29%)
		Cadherin_3	<826..882	CDD:406568	13/57 (23%)
		Cadherin_3	888..993	CDD:406568	28/105 (27%)
		Cadherin_3	1007..1114	CDD:406568	34/107 (32%)
		Cadherin_3	1132..1219	CDD:406568	20/103 (19%)
		Cadherin_3	1244..1340	CDD:406568	32/97 (33%)
		Cadherin_3	1360..1453	CDD:406568	28/94 (30%)
		CSPG 9. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01201, ECO:0000269\|PubMed:12220645	1360..1453		28/94 (30%)
		Cadherin_3	1460..1567	CDD:406568	33/107 (31%)
		CSPG 10. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01201, ECO:0000269\|PubMed:12220645	1477..1567		28/90 (31%)
		CSPG 11. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01201, ECO:0000269\|PubMed:12220645	1585..1683		25/99 (25%)
		Neurite growth inhibition	1590..2225		152/692 (22%)
		Cysteine-containing	1591..2225		152/691 (22%)
		Cadherin_3	1713..1806	CDD:406568	25/112 (22%)
		Cadherin_3	1819..1928	CDD:406568	28/121 (23%)
		Cadherin_3	2042..2127	CDD:406568	20/103 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2187..2210		7/22 (32%)
		PDZ-binding	2324..2326		1/1 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353756
Domainoid	1	1.000	75	1.000	Domainoid score	I8835
eggNOG	1	0.900	-	-		E1_KOG3597
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	555	1.000	Inparanoid score	I1071
OMA	1	1.010	-	-		QHG46545
OrthoDB	1	1.010	-	-		D119072at33208
OrthoFinder	1	1.000	-	-		FOG0003479
OrthoInspector	1	1.000	-	-		otm46034
orthoMCL	1	0.900	-	-		OOG6_106870
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X2369
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.670