DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment kon and Cspg4

DIOPT Version :9

Sequence 1:	NP_001260539.1	Gene:	kon / 35104	FlyBaseID:	FBgn0032683	Length:	2381	Species:	Drosophila melanogaster
Sequence 2:	NP_620570.2	Gene:	Cspg4 / 121021	MGIID:	2153093	Length:	2327	Species:	Mus musculus

Alignment Length:	2556	Identity:	607/2556 - (23%)
Similarity:	1051/2556 - (41%)	Gaps:	439/2556 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    17 LAILQLQTVAAMKVSLFGDGYVSMPLQEAKMSTNIRVKFRTRQENAFLFLAAGRTDYCLLRLESG 81
            ||:|...|..|   |.||:.::.:|:..|....::.::|.|.|..|.|.||||:.|:.||:|.||
Mouse    20 LALLVRSTAPA---SFFGENHLEVPVPSALTRVDLLLQFSTSQPEALLLLAAGQDDHLLLQLHSG 81

  Fly    82 LISFSYKIERDVVQLRSPKKQKLNDLEWHDVAVQRFENNITLQVDGYIMRKQLPGDLAA-LNIHF 145
            .:.....:.:..::|::|....|:|...|.|.:...::...|.||| ::....|...|: |...:
Mouse    82 CLQVRLALGQKELKLQTPADTVLSDSAPHTVVLTVSDSWAVLSVDG-VLNTSAPIPRASHLKATY 145

  Fly   146 GTFLGGVGDFTAEFLDDVI-GFRGCISDVFYNNINIIKRAKDRTSHTTSTGVAWTCSTEFEGSIQ 209
            |.|:|..|.....:|..:. ..|||:.....|..|:::        ..::.|...|:.||  |..
Mouse   146 GLFVGSSGSLDLPYLKGISRPLRGCLHSAILNGRNLLR--------PLTSDVHEGCAEEF--SAG 200

  Fly   210 DSISFMRNDSYSLMMKESYAMGE--TLSLQFRTMASAGVIFFNGG---YDFILLEIEDQHLKVTF 269
            |.:....:..:||....:::..|  ||.....|.:....:.|..|   .:||.::|.:.||:...
Mouse   201 DEVGLGFSGPHSLAAFPAWSTREEGTLEFTLTTRSQQAPLAFQAGDKRGNFIYVDIFEGHLRAVV 265

  Fly   270 NKAGSLVQFMTNEHISDGKWHRVFLRYNAAIAELSLDDATSGYRGTHANETKASINLEKSVFFGG 334
            .|....:....:..::||:.|.|.:..:....|:|:|...:   .|......:.:....|:..||
Mouse   266 EKGQGTMLLRNSVPVADGQPHEVSVHIDVHRLEISVDQYPT---RTFNRGVLSYLEPRGSLLLGG 327

  Fly   335 VQEEMRRRLISKGLRI----NEISFKGCMRDILVNDLPLGFAEMTISRSLALNCLWKYPCVEYNP 395
            :..|..|.|....|.:    ..||..||:.|..||....|..:..::|.::..|..:
Mouse   328 LDTEASRHLQEHRLGLAPGAANISLVGCIEDFSVNGRRQGLRDAWLTRDMSAGCRPE-------- 384

  Fly   396 CLKSGICSQHGVDGFICYCDQSYCIKADFQGPFKIFTETSPE----------------------- 437
                               :..|  :.:..||::.|:..:||
Mouse   385 -------------------EDEY--EEEVYGPYETFSTLAPEAWPAMELPEPCIPEPGLPAVFAN 428

  Fly   438 -LELLYVSPMQLLEGGTAFLSPHFIDIILDLRRYPSLNEQSIIFHVVHQPKYGQL----LQYSAE 497
             .:||.:||:.:.|||||:|....:...|||.. ..|.:..::|.|....::|.|    |.....
Mouse   429 FTQLLTISPLVVAEGGTAWLEWRHVQPTLDLTE-AELRKSQVLFSVSQSARHGDLELDILGAQTR 492

  Fly   498 KAIFVPCRTFNLVDLATDKLKYVHNGQENFNDHATLDMQIFGDVHKIPENILGKHRFLLHANITP 562
            |       .|.|:|:...|.::||:|.|:.:|...|::.:.... .:|..:.....::|...:.|
Mouse   493 K-------MFTLLDVVNRKARFVHDGSEDTSDQLMLEVSVTARA-PVPSCLRRGQIYILPIQVNP 549

  Fly   563 INDPPQLRLHSHKILRVIEGIERVLDVDLFNIDDPDSEPGNLIYTIL------PTQSPQETFGCF 621
            :||||::......::.::|..::.|..::|...||||....|.:.:|      |.:...:.
Mouse   550 VNDPPRIIFPHGSLMVILEHTQKPLGPEIFQAYDPDSACEGLTFQLLGVSSGVPVEHRDQP---- 610

  Fly   622 IVGGATTSAFSQAEVNVGKVSYLYNSTTAESFSYELQLQVSDGIETSETVYLP-VSVHPLELRLV 685
               |...:.||..|:.||.:.|::....|:    :|..:||||::.|....|. |:|.| .::::
Mouse   611 ---GEPATEFSCRELEVGDIVYVHRGGPAQ----DLTFRVSDGMQASAPATLKVVAVRP-AIQIL 667

  Fly   686 NNTGLIMIHKSSLPISTANLSIGTNAVDDHIDIRYDIVKAPQHGVLQRLRQIDGSWVNVDW---- 746
            :||||.:...|:..|..||||:.||||...:.:.:.:....|.|.||  :|..|.....:|
Mouse   668 HNTGLHLAQGSAAAILPANLSVETNAVGQDVSVLFRVTGTLQFGELQ--KQGAGGVEGTEWWDTL 730

  Fly   747 -FSDSQLLLGHIRYL------HSSDFPWQDEFKFIASFGFVTTQTFDFRITFTRLRITSTRPSQI 804
             |....:..|.:|||      |:.|  ..::.......|..|.....|.:|..|..:...|...:
Mouse   731 AFHQRDVEQGRVRYLSTDPQHHTQD--TVEDLILEVQVGQETLSNLSFPVTIQRATVWMLRLEPL 793

  Fly   805 SINGSREILLTSDVLS---YETTPIGS-FARSVIYKITKPTRYGGIYVEGSRKAAKKLDSFTQQD 865
            ......:..||...|.   .|....|| ...:..|::.:..|.|.:.::|:|.:..  :||:|.|
Mouse   794 HTQNPHQETLTPAHLEASLEEEEEEGSPQPHTFHYELVQAPRRGNLLLQGTRLSDG--ESFSQSD 856

  Fly   866 IEKRRIRYQTHHTSYSSFSDHLEFVVSVAECDDVAGVLEINYRPPDELINKLGYQNHEP------ 924
            ::..|:.|:....:..:..|...|.|:...          ::.|.......:|...:.|
Mouse   857 LQAGRVTYRATMRTSEAADDSFRFRVTSPP----------HFSPLYTFPIHIGGDPNAPVLTNVL 911

  Fly   925 LQVQEGERALITKNHFGIRFNIYESLQFQVSLSPEHGVICKYDEQTGLTTPVEMFTLEQLFRNDI 989
            |.|.||...:::.:|..::.....|..::|...|.||.:...|.: |.:|||..||.|.|....:
Mouse   912 LMVPEGGEGVLSADHLFVKSLNSASYLYEVMEQPHHGKLAWRDPK-GKSTPVTSFTNEDLLHGRL 975

  Fly   990 YYCHDDTESTRDTFELLIL-----SGDETDLQFVSNMEVHIKLVNDNEPYRTAIERVFHVVRNGI 1049
            .|.|||:|:..|....:..     |||....:......|.|:.|||:.|.:| |.|||||.|.|.
Mouse   976 VYQHDDSETIEDDIPFVATRQGEGSGDMAWEEVRGVFRVAIQPVNDHAPVQT-ISRVFHVARGGQ 1039

  Fly  1050 RTLNPTVLQYLDADVNTNHTDIHYIHVSSTNG---AFYKSGHYIDSFTQDDIANRRIMFQHTGAD 1111
            |.|....:.:.|||...:...:.........|   |..:....|..|||:|:..::::|.|:|||
Mouse  1040 RLLTTDDVAFSDADSGFSDAQLVLTRKDLLFGSIVAMEEPTRPIYRFTQEDLRKKQVLFVHSGAD 1104

  Fly  1112 SGTASFIVTDREHEVNGLLEIRASDPFVSMMATNASIVQEGKFVVLKNKDFILETNLDMKL-DEI 1175
            .|.....|:|.:|:...:||::||:|::.:..:::.:|.:|....:......|:||||::. :|:
Mouse  1105 HGWLQLQVSDGQHQATAMLEVQASEPYLHVANSSSLVVPQGGQGTIDTAVLQLDTNLDIRSGNEV 1169

  Fly  1176 YYEVIKPPSYGILMYLSRANEGENGTTIYKATNYTSLSNFTHLDIERERLVY-WNTEIASMDKVR 1239
            :|.|...|.:|.|:     .:|:            |:::|:..|:....::| .|..::..|.:.
Mouse  1170 HYHVTAGPQWGQLL-----RDGQ------------SVTSFSQRDLLDGAILYSHNGSLSPQDTLA 1217

  Fly  1240 YR-----VHIK-----NITAEGEVMFRIYPSAYWEPLQVKQNHTLYVEESTSVLISRDVLEVVHP 1294
            :.     ||..     .|..||       |.|   |||:.|:..:||.:..:..|.||.||||..
Mouse  1218 FSVAAGPVHTNTFLQVTIALEG-------PLA---PLQLVQHKKIYVFQGEAAEIRRDQLEVVQE 1272

  Fly  1295 NISPGDITFLVTSSPMHGYLEMQSMSFDDEY-----NCKVFDQSSVNTEKMFYIQAGVNQSTDYF 1354
            .:.|.||.|.:.|.|..|||.|.|.....|.     ..:.|.|.:||:.::.|:.:.....:|.|
Mouse  1273 AVLPADIMFSLRSPPNAGYLVMVSHGASAEEPPSLDPVQSFSQEAVNSGRVLYLHSRPGAWSDSF 1337

  Fly  1355 VFDVTNGI-TWLRQLMIKIVIIPEKLYMHSNIISVVEGKTVQLNPTDIQ---PYSEYYRGKILEY 1415
            ..||.:|: ..|..:.:::.::|..:.:.....||.||.|..|.|..:|   ||.....|.:|:
Mouse  1338 SLDVASGLGDPLEGISVELEVLPTVIPLDVQNFSVPEGGTRTLAPPLVQITGPYFPTLPGLVLQ- 1401

  Fly  1416 IVTITPSSG------HVLAGNSKVKRFTQKQLEQGSIQYVHNGSENATDSITLVAMA--RNKESV 1472
             |...|..|      |...|:  :..|:.:::|:..|:|||:|||..||:..|:|.|  .:::|.
Mouse  1402 -VLEPPQHGALQKEDHSQDGS--LSTFSWREVEEQLIRYVHDGSETQTDAFVLLANASEMDRQSQ 1463

  Fly  1473 PFELEFAVVQVNDEEPMMVTNTGLQVWNGGRYVIKNTDLLAQDYDTPPENLTFVVNHIYGGYLAR 1537
            |......::.|||:.|::.||||||:|.|....|....|...|.|:.||:|.:.:.....|.:|.
Mouse  1464 PVAFTITILPVNDQPPVLTTNTGLQIWEGAIVPIPPEALRGTDNDSGPEDLVYTIEQPSNGRIAL 1528

  Fly  1538 KSAPHQKIEHFTQAQINLEEIYFMHDSNSRRNELSFVVTDGLFNTTTQMLNIEIKPIEILA-EHN 1601
            :.||..::..|||||::...:.|.| ..:......|.::||...:......:..:...:|: |..
Mouse  1529 RVAPDTEVHRFTQAQLDSGLVLFSH-RGALEGGFHFDLSDGAHTSPGHFFRVVAQKQALLSLEGT 1592

  Fly  1602 ENLHVFPLTKKQILRDYLHFKCSD--EEREIRYNITVPPSLGRIVNEFIDNGFTKEV-SEFTQND 1663
            ..|.|.|.:.:.:....|....|.  :.|.:.|.:...|.|||:::  ...|..:|| ..|||.:
Mouse  1593 RKLTVCPESVQPLSSQSLSASSSTGADPRHLLYRVVRGPQLGRLLH--AQQGSAEEVLVNFTQAE 1655

  Fly  1664 VDNGHIFYEHTAVIMEFRTNDSFYFDVVAERSDR---LLNQKFNIEISVSSGGLLRF-------- 1717
            |:.|:|.|||.      .:::.|:     |..|.   ||:.....:::.:...::.|
Mouse  1656 VNAGNILYEHE------MSSEPFW-----EAHDTIGLLLSSPPARDLAATLAVMVSFDAACPQRP 1709

  Fly  1718 --LPVNK-LNVDEGGSVPI---KLDFSKILEYLKTKAGINNPELFIEAIQKPTHGNIGLGHEFKH 1776
              |..|| |.|.||....|   .||.:.:|..:.......:..|| :..|.||.|.:.:..|..|
Mouse  1710 SRLWKNKGLWVPEGQRAKITVAALDAANLLASVPASQRSRHDVLF-QVTQFPTRGQLLVSEEPLH 1773

  Fly  1777 MQR--YHPSDFFTKKVYYIHDHSDTLEDTILMSVYLTQG------------NIFLCNLTIPVSIN 1827
            .:|  :..|:....::.|.|....|.:|......:| ||            ..|:      :::.
Mouse  1774 ARRPYFLQSELAAGQLVYAHGGGGTQQDGFRFRAHL-QGPTGTSVAGPQTSEAFV------ITVR 1831

  Fly  1828 PINDQPFHLVTHLPQMTVVEGENRTITRNDLLTEDADTPPEEIIYDVMSGPTLGVLRKITNDGRP 1892
            .:|::|......:| :.|..|....::|..|...|.|:.|.||.|:|...|..|.|....::..|
Mouse  1832 DVNERPPQPQASIP-LRVTRGSRAPVSRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLAGDNTGP 1895

  Fly  1893 EDLLAFSNQFTQADINNDRIIYVHFGMPQSTTFCFTVSDGQSNPAYEIFTIKI--DSIHLQPSAM 1955
                  ...|||||::..|:.:|..|...:..|..::|||.|.|......:.:  .:|.:|   :
Mouse  1896 ------VTHFTQADVDAGRLAFVANGSSVAGVFQLSMSDGASPPIPMSLAVDVLPSTIEVQ---L 1951

  Fly  1956 QAPVKVQQGATTAPMRLDHIGVSTNVHMERLSYNVTNSPLFGIIVYKHQPTLRFTQQQLETSQIS 2020
            :||::|.|......:....:.|.::.....::|.:|..||:|.::...||...|:|.|::...:.
Mouse  1952 RAPLEVPQALGRTSLSRQQLQVISDREEPDVAYRLTQGPLYGQLLVGGQPASAFSQLQVDQGDVV 2016

  Fly  2021 YMQTDLNRSNDSFQVSAYVPGTNYVAQVDVVMEVEPVIQINDIVMKEAESSGGKIKLITSLDNDN 2085
            ::.|:.:.|.|.|:|.|...|.|..|.|:|.  |:.::.:         .:||.....|:|..|
Mouse  2017 FVFTNFSSSQDHFKVVALARGVNASATVNVT--VQALLHV---------WAGGPWPQGTTLRLD- 2069

  Fly  2086 PLSLKLNKF------NPKFVITRMPSTGQIRKIIRSTGLTTDGQSDRSTNIFSYKELRSGVVYF- 2143
            |..|..::.      .|.|.:...|..|   :::|.:...|:.:|::....|:.::|..|.:..
Mouse  2070 PTVLDASELANRTGSMPHFRLLAGPRYG---RVVRVSQGRTESRSNQLVEHFTQRDLEEGQLGLE 2131

  Fly  2144 VPHESVEETEADHDSFDYQLLIKTVQPAQA-------------TVSIEYRSRVE----ETE---- 2187
            |.......|....|....:|..|.|.||.|             :.|:...|..|    |||
Mouse  2132 VGKPEGRSTGPAGDRLTLELWAKGVPPAVALLDFATEPYHAAKSYSVALLSVPEAVRTETEKPGR 2196

  Fly  2188 TVHLGSAGLS-------------INYLAIGCAIFIVLICLLIVLLIL---------KIRKLRKHK 2230
            :|..|..|.:             :.:|.......|:.:||:::||.|         |..|..||.
Mouse  2197 SVPTGQPGQAASSPVPTAAKGGFLGFLEANMFSIIIPVCLILLLLALILPLLFYLRKRNKTGKHD 2261

  Fly  2231 ADISKDQPPALPCPPDLTSVSPQHHLHHHHGHHYASSEADSVPATGNSTPLPGFSNIPHCKIIPV 2295
            ..:             ||                      :.|..|.:.....|..:...:.||:
Mouse  2262 VQV-------------LT----------------------AKPRNGLAGDTETFRKVEPGQAIPL 2291

  Fly  2296 ESYKHEYPDYDPDEEETDDQCDPQQMMLPQRYNPYHMEHDAWSSSCDMANDFAGYASVPQSISGS 2360
            .:...:.|       ....|.||:.:.                           :...|
Mouse  2292 ITVPGQGP-------PPGGQPDPELLQ---------------------------FCRTP------ 2316

  Fly  2361 VSSPPSAPPTNPLLRRNQYWV 2381
                      ||.||..||||
Mouse  2317 ----------NPALRNGQYWV 2327

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
kon	NP_001260539.1	LamG	30..176	CDD:238058	41/147 (28%)
		LamG	211..365	CDD:238058	34/162 (21%)
		Cadherin_3	507..664	CDD:292802	38/162 (23%)
		Cadherin_3	631..776	CDD:292802	45/156 (29%)
		Cadherin_3	747..892	CDD:292802	32/154 (21%)
		Cadherin_3	861..1005	CDD:292802	36/149 (24%)
		Cadherin_3	979..1121	CDD:292802	47/149 (32%)
		Cadherin_3	1093..1242	CDD:292802	38/155 (25%)
		Cadherin_3	<1264..1361	CDD:292802	34/101 (34%)
		Cadherin_3	1330..1464	CDD:292802	41/143 (29%)
		Cadherin_3	1436..1578	CDD:292802	47/143 (33%)
		Cadherin_3	1789..1932	CDD:292802	36/154 (23%)
		Cadherin_3	2009..2163	CDD:292802	35/160 (22%)
Cspg4	NP_620570.2	Globular or compact configuration stabilized by disulfide bonds	30..640		150/675 (22%)
		Neurite growth inhibition. /evidence=ECO:0000250	30..640		150/675 (22%)
		LamG	55..182	CDD:304605	38/127 (30%)
		LamG	204..362	CDD:238058	34/160 (21%)
		Cadherin_3	<438..524	CDD:292802	26/93 (28%)
		Cadherin_3	495..646	CDD:292802	38/162 (23%)
		CSPG 2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01201	554..646		22/102 (22%)
		Interaction with COL6A2. /evidence=ECO:0000250	575..1045		128/499 (26%)
		Cadherin_3	617..744	CDD:292802	40/133 (30%)
		Interaction with COL5A1. /evidence=ECO:0000250	632..1451		229/872 (26%)
		Cadherin_3	732..883	CDD:292802	32/154 (21%)
		Cadherin_3	852..994	CDD:292802	36/152 (24%)
		Cadherin_3	965..1115	CDD:292802	47/150 (31%)
		Cadherin_3	1192..1341	CDD:292802	44/158 (28%)
		Cadherin_3	1313..1442	CDD:292802	35/132 (27%)
		CSPG 9. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01201	1361..1454		29/96 (30%)
		Cadherin_3	1425..1568	CDD:292802	47/143 (33%)
		CSPG 10. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01201	1478..1568		28/90 (31%)
		CSPG 11. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01201	1586..1684		29/110 (26%)
		Neurite growth inhibition. /evidence=ECO:0000250	1591..2226		155/680 (23%)
		Cysteine-containing	1592..2226		155/679 (23%)
		Cadherin_3	1651..1807	CDD:292802	40/167 (24%)
		Cadherin_3	1779..1929	CDD:292802	37/163 (23%)
		Cadherin_3	2005..2128	CDD:292802	32/137 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2190..2210		6/19 (32%)
		PDZ-binding	2325..2327		1/1 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850059
Domainoid	1	1.000	73	1.000	Domainoid score	I9211
eggNOG	1	0.900	-	-		E1_KOG3597
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	676	1.000	Inparanoid score	I723
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46545
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003479
OrthoInspector	1	1.000	-	-		otm43946
orthoMCL	1	0.900	-	-		OOG6_106870
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4086
SonicParanoid	1	1.000	-	-		X2369
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.690